WIL2-S

库存 ：999

物种来源 ：人

细胞形态 ：淋巴样

器官来源 ：脾

运输方式 ：冻存运输

年限 ：5年

生长状态 ：悬浮生长

Designations:	WIL2-S
Depositors:	Lovelace Medical Foundation
Biosafety Level:	2
Shipped:	frozen
Medium & Serum:	See Propagation
Growth Properties:	suspension
Organism:	Homo sapiens
Morphology:	lymphoblast
Source:	Organ: spleen Disease: hereditary spherocytosis Cell Type: lymphoblast B lymphoblast;
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications:	transfection host
DNA Profile (STR):	Amelogenin: X,Y CSF1PO: 11,12 D13S317: 11 D16S539: 11,12 D5S818: 12,13 D7S820: 9,11 F13A01: 6,7 F13B: 10 FESFPS: 11,12 LPL: 9,10 THO1: 8,9.3 TPOX: 8,11 vWA: 17,20
Age:	5 years
Gender:	male
Ethnicity:	Caucasian
Comments:	This is a HAT sensitive variant of the WIL2 human B cell line (see also ATCC CRL-8155).
Propagation:	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing:	Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Related Products:	WIL2-NS, ATCC CRL-8155 TK6, ATCC CRL-8015 LTR228 , ATCC HB-8502