| 出品公司: | ZYbscience |
|---|---|
| 载体名称: | PX395 或 PX395 Roseburia intestinalis Cas9 |
| 质粒类型: | CRISPR-Cas9载体；基因敲除载体；昆虫细胞载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 插入位点: | 限制性内切酶，多克隆位点 |
| 启动子: | CMV |
| 载体大小: | 10616 bp |
| 5' 测序引物及序列: | CMV Forward:CGCAAATGGGCGGTAGGCGTG |
| 3' 测序引物及序列: | C-CMV-24 (5'-TATTAGGACAAGGCTGGTGGGCAC-3') |
| 载体标签: | NLS (C ter)；HA、NLS (N ter) |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | Neomycin |
| 克隆菌株: | Stbl3 |
| 宿主细胞（系）: | 昆虫细胞 |
| 备注: | -- |
| 产品目录号: | ZY68332 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 杆状病毒 |
产品详情
文献和实验
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保存条件 ：-20℃低温保存
保质期 ：三年
英文名 ：PX395
库存 ：20
供应商 ：泽叶生物
载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS PX395 Roseburia intestinalis Cas9 10616 bp DNA SYNDEFINITION PX395 Roseburia intestinalis Cas9ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors.FEATURES Location/Qualifiers source 1..10616 /organism="PX395 Roseburia intestinalis Cas9" /mol_type="other DNA" misc_feature 47..1001 /label="Ac_5_flank" CDS complement(343..948) /label="ORF frame 3" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*" promoter 1023..1587 /label="CMV_immearly_promoter" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*" misc_feature 1090..1377 /label="CAG_enhancer" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*" misc_feature 1544..1564 /label="CMV_fwd_primer" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*" promoter 1615..1633 /label="T7_promoter" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*" misc_feature 1633..1656 /label="lacO" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*" misc_feature 1772..1796 /label="attB1" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*" CDS 1822..5307 /label="ORF frame 1" /translation="MYPYDVPDYASPKKKRKVEASMRENGSDERRRNMDEKMDYRIGL DIGIASVGWAVLQNNSDDEPVRIVDLGVRIFDTAEIPKTGESLAGPRRAARTTRRRLR RRKHRLDRIKWLFENQGLINIDDFLKRYNMAGLPDVYQLRYEALDRKLTDEELAQVLL HIAKHRGFRSTRKAETAAKENGAVLKATDENQKRMQEKGYRTVGEMIYLDEAFRTGCS WSEKGYILTPRNKAENYQHTMLRAMLVEEVKEIFSSQRRLGNEKATEELEEKYLEIMT SQRSFDLGPGMQPDGKPSPYAMEGFSDRVGKCTFLGDQGELRGAKGTYTAEYFVALQK INHTKLVNQDGETRNFTEEERRALTLLLFTQKEVKYAAVRKKLGLPEDILFYNLNYKK AATKEEQQKENQNTEKAKFIGMPYYHDYKKCLEERVKYLTENEVRDLFDEIGMILTCY KNDDSRTERLAKLGLVPIEMEGLLAYTPTKFQHLSMKAMRNIIPFLEKGMTYDKACEE AGYDFKADSKGTKQKLLTGENVNQTINEITNPVVKRSVSQTVKVINAIIRTYGSPQAI NIELAREMSKTFEERRKIKGDMEKRQKNNEDVKKQIQELGKLSPTGQDILKYRLWQEQ QGICMYSGKTIPLEELFKPGYDIDHILPYSITFDDSFRNKVLVTSQENRQKGNRTPYE YMGNDEQRWNEFETRVKTTIRDYKKQQKLLKKHFSEEERSEFKERNLTDTKYITTVIY NMIRQNLEMAPLNRPEKKKQVRAVNGAITAYLRKRWGLPQKNRETDTHHAMDAVVIAC CTDGMIQKISRYTKVRERCYSKGTEFVDAETGEIFRPEDYSRAEWDEIFGVHIPKPWE TFRAELDVRMGDDPKGFLDTHSDVALELDYPEYIYENLRPIFVSRMPNHKVTGAAHAD TIRSPRHFKDEGIVLTKTALTDLKLDKDGEIDGYYNPQSDLLLYEALKKQLLLYGNDA KKAFAQDFHKPKADGTEGPVVRKVKIQKKQTMGVFVDSGNGIAENGGMVRIDVFRVNG KYYFVPVYTADVVKKVLPNRASTAHKPYGEWKVMEDKDFLFSLYSRDLIHIKSKKDIP IKMVNGGMEGIKETYAYYIGADISAANIQGIAHDSRYKFRGLGIQSLDVLEKCQIDVL GHVSVVRSEKRMGFS*" misc_feature 1825..1851 /label="HA" /translation="MYPYDVPDYASPKKKRKVEASMRENGSDERRRNMDEKMDYRIGL DIGIASVGWAVLQNNSDDEPVRIVDLGVRIFDTAEIPKTGESLAGPRRAARTTRRRLR RRKHRLDRIKWLFENQGLINIDDFLKRYNMAGLPDVYQLRYEALDRKLTDEELAQVLL HIAKHRGFRSTRKAETAAKENGAVLKATDENQKRMQEKGYRTVGEMIYLDEAFRTGCS WSEKGYILTPRNKAENYQHTMLRAMLVEEVKEIFSSQRRLGNEKATEELEEKYLEIMT SQRSFDLGPGMQPDGKPSPYAMEGFSDRVGKCTFLGDQGELRGAKGTYTAEYFVALQK INHTKLVNQDGETRNFTEEERRALTLLLFTQKEVKYAAVRKKLGLPEDILFYNLNYKK AATKEEQQKENQNTEKAKFIGMPYYHDYKKCLEERVKYLTENEVRDLFDEIGMILTCY KNDDSRTERLAKLGLVPIEMEGLLAYTPTKFQHLSMKAMRNIIPFLEKGMTYDKACEE AGYDFKADSKGTKQKLLTGENVNQTINEITNPVVKRSVSQTVKVINAIIRTYGSPQAI NIELAREMSKTFEERRKIKGDMEKRQKNNEDVKKQIQELGKLSPTGQDILKYRLWQEQ QGICMYSGKTIPLEELFKPGYDIDHILPYSITFDDSFRNKVLVTSQENRQKGNRTPYE YMGNDEQRWNEFETRVKTTIRDYKKQQKLLKKHFSEEERSEFKERNLTDTKYITTVIY NMIRQNLEMAPLNRPEKKKQVRAVNGAITAYLRKRWGLPQKNRETDTHHAMDAVVIAC CTDGMIQKISRYTKVRERCYSKGTEFVDAETGEIFRPEDYSRAEWDEIFGVHIPKPWE TFRAELDVRMGDDPKGFLDTHSDVALELDYPEYIYENLRPIFVSRMPNHKVTGAAHAD TIRSPRHFKDEGIVLTKTALTDLKLDKDGEIDGYYNPQSDLLLYEALKKQLLLYGNDA KKAFAQDFHKPKADGTEGPVVRKVKIQKKQTMGVFVDSGNGIAENGGMVRIDVFRVNG KYYFVPVYTADVVKKVLPNRASTAHKPYGEWKVMEDKDFLFSLYSRDLIHIKSKKDIP IKMVNGGMEGIKETYAYYIGADISAANIQGIAHDSRYKFRGLGIQSLDVLEKCQIDVL GHVSVVRSEKRMGFS*" misc_feature 1855..1875 /label="SV40 NLS" /translation="MYPYDVPDYASPKKKRKVEASMRENGSDERRRNMDEKMDYRIGL DIGIASVGWAVLQNNSDDEPVRIVDLGVRIFDTAEIPKTGESLAGPRRAARTTRRRLR RRKHRLDRIKWLFENQGLINIDDFLKRYNMAGLPDVYQLRYEALDRKLTDEELAQVLL HIAKHRGFRSTRKAETAAKENGAVLKATDENQKRMQEKGYRTVGEMIYLDEAFRTGCS WSEKGYILTPRNKAENYQHTMLRAMLVEEVKEIFSSQRRLGNEKATEELEEKYLEIMT SQRSFDLGPGMQPDGKPSPYAMEGFSDRVGKCTFLGDQGELRGAKGTYTAEYFVALQK INHTKLVNQDGETRNFTEEERRALTLLLFTQKEVKYAAVRKKLGLPEDILFYNLNYKK AATKEEQQKENQNTEKAKFIGMPYYHDYKKCLEERVKYLTENEVRDLFDEIGMILTCY KNDDSRTERLAKLGLVPIEMEGLLAYTPTKFQHLSMKAMRNIIPFLEKGMTYDKACEE AGYDFKADSKGTKQKLLTGENVNQTINEITNPVVKRSVSQTVKVINAIIRTYGSPQAI NIELAREMSKTFEERRKIKGDMEKRQKNNEDVKKQIQELGKLSPTGQDILKYRLWQEQ QGICMYSGKTIPLEELFKPGYDIDHILPYSITFDDSFRNKVLVTSQENRQKGNRTPYE YMGNDEQRWNEFETRVKTTIRDYKKQQKLLKKHFSEEERSEFKERNLTDTKYITTVIY NMIRQNLEMAPLNRPEKKKQVRAVNGAITAYLRKRWGLPQKNRETDTHHAMDAVVIAC CTDGMIQKISRYTKVRERCYSKGTEFVDAETGEIFRPEDYSRAEWDEIFGVHIPKPWE TFRAELDVRMGDDPKGFLDTHSDVALELDYPEYIYENLRPIFVSRMPNHKVTGAAHAD TIRSPRHFKDEGIVLTKTALTDLKLDKDGEIDGYYNPQSDLLLYEALKKQLLLYGNDA KKAFAQDFHKPKADGTEGPVVRKVKIQKKQTMGVFVDSGNGIAENGGMVRIDVFRVNG KYYFVPVYTADVVKKVLPNRASTAHKPYGEWKVMEDKDFLFSLYSRDLIHIKSKKDIP IKMVNGGMEGIKETYAYYIGADISAANIQGIAHDSRYKFRGLGIQSLDVLEKCQIDVL GHVSVVRSEKRMGFS*" misc_feature 1861..1875 /label="NLS" /translation="MYPYDVPDYASPKKKRKVEASMRENGSDERRRNMDEKMDYRIGL DIGIASVGWAVLQNNSDDEPVRIVDLGVRIFDTAEIPKTGESLAGPRRAARTTRRRLR RRKHRLDRIKWLFENQGLINIDDFLKRYNMAGLPDVYQLRYEALDRKLTDEELAQVLL HIAKHRGFRSTRKAETAAKENGAVLKATDENQKRMQEKGYRTVGEMIYLDEAFRTGCS WSEKGYILTPRNKAENYQHTMLRAMLVEEVKEIFSSQRRLGNEKATEELEEKYLEIMT SQRSFDLGPGMQPDGKPSPYAMEGFSDRVGKCTFLGDQGELRGAKGTYTAEYFVALQK INHTKLVNQDGETRNFTEEERRALTLLLFTQKEVKYAAVRKKLGLPEDILFYNLNYKK AATKEEQQKENQNTEKAKFIGMPYYHDYKKCLEERVKYLTENEVRDLFDEIGMILTCY KNDDSRTERLAKLGLVPIEMEGLLAYTPTKFQHLSMKAMRNIIPFLEKGMTYDKACEE AGYDFKADSKGTKQKLLTGENVNQTINEITNPVVKRSVSQTVKVINAIIRTYGSPQAI NIELAREMSKTFEERRKIKGDMEKRQKNNEDVKKQIQELGKLSPTGQDILKYRLWQEQ QGICMYSGKTIPLEELFKPGYDIDHILPYSITFDDSFRNKVLVTSQENRQKGNRTPYE YMGNDEQRWNEFETRVKTTIRDYKKQQKLLKKHFSEEERSEFKERNLTDTKYITTVIY NMIRQNLEMAPLNRPEKKKQVRAVNGAITAYLRKRWGLPQKNRETDTHHAMDAVVIAC CTDGMIQKISRYTKVRERCYSKGTEFVDAETGEIFRPEDYSRAEWDEIFGVHIPKPWE TFRAELDVRMGDDPKGFLDTHSDVALELDYPEYIYENLRPIFVSRMPNHKVTGAAHAD TIRSPRHFKDEGIVLTKTALTDLKLDKDGEIDGYYNPQSDLLLYEALKKQLLLYGNDA KKAFAQDFHKPKADGTEGPVVRKVKIQKKQTMGVFVDSGNGIAENGGMVRIDVFRVNG KYYFVPVYTADVVKKVLPNRASTAHKPYGEWKVMEDKDFLFSLYSRDLIHIKSKKDIP IKMVNGGMEGIKETYAYYIGADISAANIQGIAHDSRYKFRGLGIQSLDVLEKCQIDVL GHVSVVRSEKRMGFS*" CDS complement(1999..3270) /label="ORF frame 3" /translation="MLEFGRSISQQAFHLDGHQSQLGQSFGAGIVIFVTSQNHSDLIK QVPDFVLGQILHSLFQAFLVIVIVWHPDKLGFFGILVLLLLLFFSSGLLVVQVVEQDV LRQAQFLADCSVLHLFLGKQQQSQGSPLFLSEIPCLAVLIDQLCVVDFLQSHEIFGCV GPLSAPQLSLIPQKSTFPHSVREALHCIRGGLPIWLHSRPQVKRALGGHDLQVLFLQF LSGFFVAQSALGAENLLDFLHQHCPQHCVLIIFSLVAGGQDVPFLTPRAASPEGLVQV DHLAHCPVSLFLHSFLILVCGLQHCPVFLGCCLGFPGASEAPMLSDVQQHLGQFLIGQ FSVQSLIPQLVHIWQSGHVVSLQKIVNIDQPLVLEQPLDSVQAVFPSPQSAPCGSSCS PGSCQAFACLWDLCGIENAHSQVDNPHRLIV*" CDS complement(4276..4764) /label="ORF frame 3" /translation="MKVLSEGLFGIIAIQQQLLFQCFVQQQIGLGVIVSVDLPILVQF QVGQCGLSQHDALIFKVSWGPNCVSMGCSGDLVIWHSAHEDRPQVFVDVLGIIQLQSH IAMSVQESLWIVPHSDVQFGAKCLPGLWDVHAENFIPLGPAVVLGSKDFASLCIDELC SL*" misc_feature complement(5340..5357) /label="Sp6_primer" /translation="MKVLSEGLFGIIAIQQQLLFQCFVQQQIGLGVIVSVDLPILVQF QVGQCGLSQHDALIFKVSWGPNCVSMGCSGDLVIWHSAHEDRPQVFVDVLGIIQLQSH IAMSVQESLWIVPHSDVQFGAKCLPGLWDVHAENFIPLGPAVVLGSKDFASLCIDELC SL*" misc_feature 5362..5386 /label="attB2" /translation="MKVLSEGLFGIIAIQQQLLFQCFVQQQIGLGVIVSVDLPILVQF QVGQCGLSQHDALIFKVSWGPNCVSMGCSGDLVIWHSAHEDRPQVFVDVLGIIQLQSH IAMSVQESLWIVPHSDVQFGAKCLPGLWDVHAENFIPLGPAVVLGSKDFASLCIDELC SL*" terminator 5398..5864 /label="hGH_PA_terminator" /translation="MKVLSEGLFGIIAIQQQLLFQCFVQQQIGLGVIVSVDLPILVQF QVGQCGLSQHDALIFKVSWGPNCVSMGCSGDLVIWHSAHEDRPQVFVDVLGIIQLQSH IAMSVQESLWIVPHSDVQFGAKCLPGLWDVHAENFIPLGPAVVLGSKDFASLCIDELC SL*" terminator 5870..5905 /label="T7_terminator" /translation="MKVLSEGLFGIIAIQQQLLFQCFVQQQIGLGVIVSVDLPILVQF QVGQCGLSQHDALIFKVSWGPNCVSMGCSGDLVIWHSAHEDRPQVFVDVLGIIQLQSH IAMSVQESLWIVPHSDVQFGAKCLPGLWDVHAENFIPLGPAVVLGSKDFASLCIDELC SL*" rep_origin 6022..6328 /label="f1_origin" /translation="MKVLSEGLFGIIAIQQQLLFQCFVQQQIGLGVIVSVDLPILVQF QVGQCGLSQHDALIFKVSWGPNCVSMGCSGDLVIWHSAHEDRPQVFVDVLGIIQLQSH IAMSVQESLWIVPHSDVQFGAKCLPGLWDVHAENFIPLGPAVVLGSKDFASLCIDELC SL*" misc_feature complement(6442..6462) /label="pBABE_3_primer" /translation="MKVLSEGLFGIIAIQQQLLFQCFVQQQIGLGVIVSVDLPILVQF QVGQCGLSQHDALIFKVSWGPNCVSMGCSGDLVIWHSAHEDRPQVFVDVLGIIQLQSH IAMSVQESLWIVPHSDVQFGAKCLPGLWDVHAENFIPLGPAVVLGSKDFASLCIDELC SL*" misc_feature complement(6448..6663) /label="SV40_enhancer" /translation="MKVLSEGLFGIIAIQQQLLFQCFVQQQIGLGVIVSVDLPILVQF QVGQCGLSQHDALIFKVSWGPNCVSMGCSGDLVIWHSAHEDRPQVFVDVLGIIQLQSH IAMSVQESLWIVPHSDVQFGAKCLPGLWDVHAENFIPLGPAVVLGSKDFASLCIDELC SL*" promoter 6460..6728 /label="SV40_promoter" /translation="MKVLSEGLFGIIAIQQQLLFQCFVQQQIGLGVIVSVDLPILVQF QVGQCGLSQHDALIFKVSWGPNCVSMGCSGDLVIWHSAHEDRPQVFVDVLGIIQLQSH IAMSVQESLWIVPHSDVQFGAKCLPGLWDVHAENFIPLGPAVVLGSKDFASLCIDELC SL*" rep_origin 6627..6704 /label="SV40_origin" /translation="MKVLSEGLFGIIAIQQQLLFQCFVQQQIGLGVIVSVDLPILVQF QVGQCGLSQHDALIFKVSWGPNCVSMGCSGDLVIWHSAHEDRPQVFVDVLGIIQLQSH IAMSVQESLWIVPHSDVQFGAKCLPGLWDVHAENFIPLGPAVVLGSKDFASLCIDELC SL*" misc_feature 6689..6708 /label="SV40pro_F_primer" /translation="MKVLSEGLFGIIAIQQQLLFQCFVQQQIGLGVIVSVDLPILVQF QVGQCGLSQHDALIFKVSWGPNCVSMGCSGDLVIWHSAHEDRPQVFVDVLGIIQLQSH IAMSVQESLWIVPHSDVQFGAKCLPGLWDVHAENFIPLGPAVVLGSKDFASLCIDELC SL*" CDS 6843..7637 /label="ORF frame 3" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" gene 6846..7634 /label="NeoR/KanR" /gene="NeoR/KanR" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" CDS complement(7152..7688) /label="ORF frame 1" /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ PPHCISHDGYFLGRSKVR*" terminator 7814..7933 /label="SV40_PA_terminator" /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ PPHCISHDGYFLGRSKVR*" misc_feature 7902..7921 /label="EBV_rev_primer" /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ PPHCISHDGYFLGRSKVR*" terminator 8124..8159 /label="T7_terminator" /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ PPHCISHDGYFLGRSKVR*" misc_feature complement(8182..8199)
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