pCambia1381Xb载体

LOCUS       pCAMBIA1381Xb          10647 bp ds-DNA    circular SYN 01-十二月-2013DEFINITION  Agrobacterium binary vector with hygromycin- and             kanamycin-resistance and GUS genes plus the pUC8 MCS. For other             reading frames, use pCAMBIA1381Xa or pCAMBIA1381Xc.ACCESSION   AF234304VERSION     .KEYWORDS    pCAMBIA1381XbSOURCE      synthetic DNA construct  ORGANISM  synthetic DNA constructCOMMENT     This file is created by Vector NTI            http://www.biofeng.com/COMMENT     VNTAUTHORNAME|biofeng.com|COMMENT     The GenBank record was corrected by inserting a G at position 4541.FEATURES             Location/Qualifiers     source          1..10647                     /organism="synthetic DNA construct"                     /lab_host="Plant Cells"                     /mol_type="other DNA"     CDS             20..1825                     /codon_start=1                     /gene="uidA?(or gusA)?from E. coli"                     /product="beta-glucuronidase"                     /label="GUS"     CDS             1832..1849                     /codon_start=1                     /product="6xHis affinity tag"                     /label="6xHis"                     /translation="HHHHHH"     terminator      1881..2133                     /label="NOS terminator"                     /label="nopaline synthase terminator and poly(A) signal"     misc_feature    2155..2179                     /label="RB T-DNA repeat"                     /label="right border repeat from nopaline C58 T-DNA"     CDS             3479..4108                     /codon_start=1                     /product="stability protein from Pseudomonas plasmid pVS1"                     /label="pVS1 StaA"     CDS             4537..5610                     /codon_start=1                     /product="replication protein from Pseudomonas plasmid                      pVS1"                     /label="pVS1 RepA"                     /label="                     "                     /protein_id="                     "     misc_feature    6214..6354                     /label="bom"                     /label="basis of mobility region from pBR322"     rep_origin      complement(6540..7128)                     /direction=LEFT                     /label="ori"                     /label="high-copy-number ColE1/pMB1/pBR322/pUC origin of                      replication"     CDS             complement(7215..8009)                     /codon_start=1                     /gene="aphA-3"                     /product="aminoglycoside phosphotransferase"                     /label="KanR"                     /label="confers resistance to kanamycin"                     /protein_id="                 "                          misc_feature    8434..8458                     /label="LB T-DNA repeat"                     /label="left border repeat from nopaline C58 T-DNA"     polyA_signal    8536..8710                     /label="CaMV poly(A) signal"                     /label="cauliflower mosaic virus polyadenylation signal"     CDS             complement(8750..9775)                     /codon_start=1                     /product="hygromycin B phosphotransferase"                     /label="HygR"                     /label="confers resistance to hygromycin"                          promoter        complement(9843..10520)                     /label="CaMV 35S promoter (enhanced)"                     /label="cauliflower mosaic virus 35S promoter with a                      duplicated enhancer region"     misc_feature    10609..10644                     /label="MCS"                     /label="multiple cloning site from pUC8"ORIGIN        1 cgctgtagat ctgactagtt tacgtcctgt agaaacccca acccgtgaaa tcaaaaaact       61 cgacggcctg tgggcattca gtctggatcg cgaaaactgt ggaattgatc agcgttggtg      121 ggaaagcgcg ttacaagaaa gccgggcaat tgctgtgcca ggcagtttta acgatcagtt      181 cgccgatgca gatattcgta attatgcggg caacgtctgg tatcagcgcg aagtctttat      241 accgaaaggt tgggcaggcc agcgtatcgt gctgcgtttc gatgcggtca ctcattacgg      301 caaagtgtgg gtcaataatc aggaagtgat ggagcatcag ggcggctata cgccatttga      361 agccgatgtc acgccgtatg ttattgccgg gaaaagtgta cgtatcaccg tttgtgtgaa      421 caacgaactg aactggcaga ctatcccgcc gggaatggtg attaccgacg aaaacggcaa      481 gaaaaagcag tcttacttcc atgatttctt taactatgcc ggaatccatc gcagcgtaat      541 gctctacacc acgccgaaca cctgggtgga cgatatcacc gtggtgacgc atgtcgcgca      601 agactgtaac cacgcgtctg ttgactggca ggtggtggcc aatggtgatg tcagcgttga      661 actgcgtgat gcggatcaac aggtggttgc aactggacaa ggcactagcg ggactttgca      721 agtggtgaat ccgcacctct ggcaaccggg tgaaggttat ctctatgaac tgtgcgtcac      781 agccaaaagc cagacagagt gtgatatcta cccgcttcgc gtcggcatcc ggtcagtggc      841 agtgaagggc caacagttcc tgattaacca caaaccgttc tactttactg gctttggtcg      901 tcatgaagat gcggacttac gtggcaaagg attcgataac gtgctgatgg tgcacgacca      961 cgcattaatg gactggattg gggccaactc ctaccgtacc tcgcattacc cttacgctga     1021 agagatgctc gactgggcag atgaacatgg catcgtggtg attgatgaaa ctgctgctgt     1081 cggctttcag ctgtctttag gcattggttt cgaagcgggc aacaagccga aagaactgta     1141 cagcgaagag gcagtcaacg gggaaactca gcaagcgcac ttacaggcga ttaaagagct     1201 gatagcgcgt gacaaaaacc acccaagcgt ggtgatgtgg agtattgcca acgaaccgga     1261 tacccgtccg caaggtgcac gggaatattt cgcgccactg gcggaagcaa cgcgtaaact     1321 cgacccgacg cgtccgatca cctgcgtcaa tgtaatgttc tgcgacgctc acaccgatac     1381 catcagcgat ctctttgatg tgctgtgcct gaaccgttat tacggatggt atgtccaaag     1441 cggcgatttg gaaacggcag agaaggtact ggaaaaagaa cttctggcct ggcaggagaa     1501 actgcatcag ccgattatca tcaccgaata cggcgtggat acgttagccg ggctgcactc     1561 aatgtacacc gacatgtgga gtgaagagta tcagtgtgca tggctggata tgtatcaccg     1621 cgtctttgat cgcgtcagcg ccgtcgtcgg tgaacaggta tggaatttcg ccgattttgc     1681 gacctcgcaa ggcatattgc gcgttggcgg taacaagaaa gggatcttca ctcgcgaccg     1741 caaaccgaag tcggcggctt ttctgctgca aaaacgctgg actggcatga acttcggtga     1801 aaaaccgcag cagggaggca aacaagctag ccaccaccac caccaccacg tgtgaattgg     1861 tgaccagctc gaatttcccc gatcgttcaa acatttggca ataaagtttc ttaagattga     1921 atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg     1981 taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc     2041 cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat     2101 tatcgcgcgc ggtgtcatct atgttactag atcgggaatt aaactatcag tgtttgacag     2161 gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataacg gatatttaaa     2221 agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acagggttcc     2281 cctcgggatc aaagtacttt gatccaaccc ctccgctgct atagtgcagt cggcttctga     2341 cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag     2401 gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag     2461 aatacttgcg actagaaccg gagacattac gccatgaaca agagcgccgc cgctggcctg     2521 ctgggctatg cccgcgtcag caccgacgac caggacttga ccaaccaacg ggccgaactg     2581 cacgcggccg gctgcaccaa gctgttttcc gagaagatca ccggcaccag gcgcgaccgc     2641 ccggagctgg ccaggatgct tgaccaccta cgccctggcg acgttgtgac agtgaccagg     2701 ctagaccgcc tggcccgcag cacccgcgac ctactggaca ttgccgagcg catccaggag     2761 gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg acaccaccac gccggccggc     2821 cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg agcgttccct aatcatcgac     2881 cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg cccccgccct     2941 accctcaccc cggcacagat cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc     3001 gtgaaagagg cggctgcact gcttggcgtg catcgctcga ccctgtaccg cgcacttgag     3061 cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg tgaggacgca     3121 ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac gccaagagga acaagcatga     3181 aaccgcacca ggacggccag gacgaaccgt ttttcattac cgaagagatc gaggcggaga     3241 tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt ctcaaccgtg cggctgcatg     3301 aaatcctggc cggtttgtct gatgccaagc tggcggcctg gccggccagc ttggccgctg     3361 aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc     3421 atgcggtcgc tgcgtatatg atgcgatgag taaataaaca aatacgcaag gggaacgcat     3481 gaaggttatc gctgtactta accagaaagg cgggtcaggc aagacgacca tcgcaaccca     3541 tctagcccgc gccctgcaac tcgccggggc cgatgttctg ttagtcgatt ccgatcccca     3601 gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat     3661 cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat     3721 cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt     3781 gctgattccg gtgcagccaa gcccttacga catatgggcc accgccgacc tggtggagct     3841 ggttaagcag cgcattgagg tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg     3901 ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag gcgctggccg ggtacgagct     3961 gcccattctt gagtcccgta tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg     4021 cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc     4081 cgctgaaatt aaatcaaaac tcatttgagt taatgaggta aagagaaaat gagcaaaagc     4141 acaaacacgc taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc     4201 agcctggcag acacgccagc catgaagcgg gtcaactttc agttgccggc ggaggatcac     4261 accaagctga agatgtacgc ggtacgccaa ggcaagacca ttaccgagct gctatctgaa     4321 tacatcgcgc agctaccaga gtaaatgagc aaatgaataa atgagtagat gaattttagc     4381 ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc accgacgccg tggaatgccc     4441 catgtgtgga ggaacgggcg gttggccagg cgtaagcggc tgggttgtct gccggccctg     4501 caatggcact ggaaccccca agcccgagga atcggcgtga gcggtcgcaa accatccggc     4561 ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg agaagttgaa ggccgcgcag     4621 gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc     4681 gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg     4741 aagccgccca agggcgacga gcaaccagat tttttcgttc cgatgctcta tgacgtgggc     4801 acccgcgata gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga     4861 cgagctggcg aggtgatccg ctacgagctt ccagacgggc acgtagaggt ttccgcaggg     4921 ccggccggca tggccagtgt gtgggattac gacctggtac tgatggcggt ttcccatcta     4981 accgaatcca tgaaccgata ccgggaaggg aagggagaca agcccggccg cgtgttccgt     5041 ccacacgttg cggacgtact caagttctgc cggcgagccg atggcggaaa gcagaaagac     5101 gacctggtag aaacctgcat tcggttaaac accacgcacg ttgccatgca gcgtacgaag     5161 aaggccaaga acggccgcct ggtgacggta tccgagggtg aagccttgat tagccgctac     5221 aagatcgtaa agagcgaaac cgggcggccg gagtacatcg agatcgagct agctgattgg     5281 atgtaccgcg agatcacaga aggcaagaac ccggacgtgc tgacggttca ccccgattac     5341 tttttgatcg atcccggcat cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc     5401 aaggcagaag ccagatggtt gttcaagacg atctacgaac gcagtggcag cgccggagag     5461 ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct gccggagtac     5521 gatttgaagg aggaggcggg gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg     5581 atcgagggcg aagcatccgc cggttcctaa tgtacggagc agatgctagg gcaaattgcc     5641 ctagcagggg aaaaaggtcg aaaaggtctc tttcctgtgg atagcacgta cattgggaac     5701 ccaaagccgt acattgggaa ccggaacccg tacattggga acccaaagcc gtacattggg     5761 aaccggtcac acatgtaagt gactgatata aaagagaaaa aaggcgattt ttccgcctaa     5821 aactctttaa aacttattaa aactcttaaa acccgcctgg cctgtgcata actgtctggc     5881 cagcgcacag ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc     5941 cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc tggcctacgg     6001 ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca     6061 catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca     6121 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca     6181 gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga     6241 tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac     6301 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct     6361 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca     6421 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac     6481 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt     6541 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg     6601 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc     6661 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc     6721 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc     6781 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac     6841 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt     6901 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct     6961 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc     7021 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt     7081 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg     7141 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc     7201 atgcattcta ggtactaaaa caattcatcc agtaaaatat aatattttat tttctcccaa     7261 tcaggcttga tccccagtaa gtcaaaaaat agctcgacat actgttcttc cccgatatcc     7321 tccctgatcg accggacgca gaaggcaatg tcataccact tgtccgccct gccgcttctc     7381 ccaagatcaa taaagccact tactttgcca tctttcacaa agatgttgct gtctcccagg     7441 tcgccgtggg aaaagacaag ttcctcttcg ggcttttccg tctttaaaaa atcatacagc