pHANNIBAL载体

LOCUS       pHannibal           5824 bp ds-DNA    circular SYN 18-5月-2013DEFINITION  Vector for silencing genes in plants using intron-containing hairpin            RNA (hpRNA).ACCESSION   AJ311872VERSION     .KEYWORDS    pHANNIBALSOURCE      synthetic DNA construct  ORGANISM  synthetic DNA constructREFERENCE   1  (bases 1 to 5824)  AUTHORS   Wesley SV, Helliwell CA, Smith NA, Wang MB, Rouse DT, Liu Q, Gooding            PS, Singh SP, Abbott D, Stoutjesdijk PA, Robinson SP, Gleave AP,             Green AG, Waterhouse PM.  TITLE     Construct design for efficient, effective and high-throughput gene             silencing in plants.  JOURNAL   Plant J. 2001;27:581-90.  PUBMED    11576441REFERENCE   2  (bases 1 to 5824)  AUTHORS   .  TITLE     Direct Submission  JOURNAL   Exported 2016年3月7日 from SnapGene 1.1.3            http://www.snapgene.comFEATURES             Location/Qualifiers     source          1..5824                     /organism="synthetic DNA construct"                     /lab_host="Plant Cells"                     /mol_type="other DNA"     rep_origin      complement(215..643)                     /direction=LEFT                     /note="f1 ori"                     /note="color: #ffff00"     promoter        670..774                     /note="AmpR promoter"                     /note="color: #ffffff; direction: RIGHT"     CDS             775..1635                     /codon_start=1                     /note="AmpR"                     /note="This feature has 2 segments:                     ???1:??775?..??843?/?#ccffcc?/?signal?sequence                     ???2:??844?..?1635?/?#ccffcc                     Cleavage site after base 843"                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS                     LIKHW"     rep_origin      1806..2394                     /direction=RIGHT                     /note="ori"                     /note="color: #ffff00"     promoter        3863..4208                     /note="CaMV 35S promoter"                     /note="color: #ffffff; direction: RIGHT"     misc_feature    4210..4228                     /note="MCS 1"                     /note="color: #99ccff"     intron          4236..5002                     /note="PDK intron"                     /note="color: #------"     misc_feature    5022..5045                     /note="MCS 2"                     /note="color: #99ccff"     terminator      5049..5755                     /note="OCS terminator"                     /note="color: #ffffff"ORIGIN        1 tgaccaagtc agcttggcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg       61 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga      121 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggaaat      181 tgtaaacgtt aatattttgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc      241 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag      301 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg      361 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca      421 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa      481 gggatgcccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg      541 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta      601 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg      661 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg      721 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt      781 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt      841 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg      901 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa      961 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt     1021 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag     1081 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt     1141 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga     1201 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt     1261 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta     1321 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg     1381 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc     1441 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt     1501 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg     1561 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg     1621 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa     1681 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa     1741 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga     1801 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg     1861 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact     1921 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac     1981 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg     2041 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg     2101 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga     2161 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc     2221 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg     2281 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc     2341 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc     2401 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt     2461 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc     2521 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc     2581 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac     2641 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact     2701 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg     2761 agcggataac aatttcacac aggaaacagc tatgaccatg attacgaatt tggccaagtc     2821 ggcctctaat acgactcact atagggagct cgtcgagcgg ccgctcgacg aattaattcc     2881 aatcccacaa aaatctgagc ttaacagcac agttgctcct ctcagagcag aatcgggtat     2941 tcaacaccct catatcaact actacgttgt gtataacggt ccacatgccg gtatatacga     3001 tgactggggt tgtacaaagg cggcaacaaa cggcgttccc ggagttgcac acaagaaatt     3061 tgccactatt acagaggcaa gagcagcagc tgacgcgtac acaacaagtc agcaaacaga     3121 caggttgaac ttcatcccca aaggagaagc tcaactcaag cccaagagct ttgctaaggc     3181 cctaacaagc ccaccaaagc aaaaagccca ctggctcacg ctaggaacca aaaggcccag     3241 cagtgatcca gccccaaaag agatctcctt tgccccggag attacaatgg acgatttcct     3301 ctatctttac gatctaggaa ggaagttcga aggtgaaggt gacgacacta tgttcaccac     3361 tgataatgag aaggttagcc tcttcaattt cagaaagaat gctgacccac agatggttag     3421 agaggcctac gcagcaggtc tcatcaagac gatctacccg agtaacaatc tccaggagat     3481 caaatacctt cccaagaagg ttaaagatgc agtcaaaaga ttcaggacta attgcatcaa     3541 gaacacagag aaagacatat ttctcaagat cagaagtact attccagtat ggacgattca     3601 aggcttgctt cataaaccaa ggcaagtaat agagattgga gtctctaaaa aggtagttcc     3661 tactgaatct aaggccatgc atggagtcta agattcaaat cgaggatcta acagaactcg     3721 ccgtgaagac tggcgaacag ttcatacaga gtcttttacg actcaatgac aagaagaaaa     3781 tcttcgtcaa catggtggag cacgacactc tggtctactc caaaaatgtc aaagatacag     3841 tctcagaaga ccaaagggct attgagactt ttcaacaaag gataatttcg ggaaacctcc     3901 tcggattcca ttgcccagct atctgtcact tcatcgaaag gacagtagaa aaggaaggtg     3961 gctcctacaa atgccatcat tgcgataaag gaaaggctat cattcaagat ctctctgccg     4021 acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagacgttc     4081 caaccacgtc ttcaaagcaa gtggattgat gtgacatctc cactgacgta agggatgacg     4141 cacaatccca ctatccttcg caagaccctt cctctatata aggaagttca tttcatttgg     4201 agaggacacg ctcgaggaat tcggtacccc aattggtaag gaaataatta ttttcttttt     4261 tccttttagt ataaaatagt taagtgatgt taattagtat gattataata atatagttgt     4321 tataattgtg aaaaaataat ttataaatat attgtttaca taaacaacat agtaatgtaa     4381 aaaaatatga caagtgatgt gtaagacgaa gaagataaaa gttgagagta agtatattat     4441 ttttaatgaa tttgatcgaa catgtaagat gatatactag cattaatatt tgttttaatc     4501 ataatagtaa ttctagctgg tttgatgaat taaatatcaa tgataaaata ctatagtaaa     4561 aataagaata aataaattaa aataatattt ttttatgatt aatagtttat tatataatta     4621 aatatctata ccattactaa atattttagt ttaaaagtta ataaatattt tgttagaaat     4681 tccaatctgc ttgtaattta tcaataaaca aaatattaaa taacaagcta aagtaacaaa     4741 taatatcaaa ctaatagaaa cagtaatcta atgtaacaaa acataatcta atgctaatat     4801 aacaaagcgc aagatctatc attttatata gtattatttt caatcaacat tcttattaat     4861 ttctaaataa tacttgtagt tttattaact tctaaatgga ttgactatta attaaatgaa     4921 ttagtcgaac atgaataaac aaggtaacat gatagatcat gtcattgtgt tatcattgat     4981 cttacatttg gattgattac agttgggaaa ttgggttcga aatcgataag cttggatcct     5041 ctagagtcct gctttaatga gatatgcgag acgcctatga tcgcatgata tttgctttca     5101 attctgttgt gcacgttgta aaaaacctga gcatgtgtag ctcagatcct taccgccggt     5161 ttcggttcat tctaatgaat atatcacccg ttactatcgt atttttatga ataatattct     5221 ccgttcaatt tactgattgt accctactac ttatatgtac aatattaaaa tgaaaacaat     5281 atattgtgct gaataggttt atagcgacat ctatgataga gcgccacaat aacaaacaat     5341 tgcgttttat tattacaaat ccaattttaa aaaaagcggc agaaccggtc aaacctaaaa     5401 gactgattac ataaatctta ttcaaatttc aaaaggcccc aggggctagt atctacgaca     5461 caccgagcgg cgaactaata acgttcactg aagggaactc cggttccccg ccggcgcgca     5521 tgggtgagat tccttgaagt tgagtattgg ccgtccgctc taccgaaagt tacgggcacc     5581 attcaacccg gtccagcacg gcggccgggt aaccgacttg ctgccccgag aattatgcag     5641 catttttttg gtgtatgtgg gccccaaatg aagtgcaggt caaaccttga cagtgacgac     5701 aaatcgttgg gcgggtccag ggcgaatttt gcgacaacat gtcgaggctc agcaggacct     5761 gcaggcatgc aagctagctt actagtgatg catattctat agtgtcacct aaatctgcgg     5821 ccgc//