pENTR-GUS载体

LOCUS       Exported                3841 bp ds-DNA     circular SYN 13-AUG-2016DEFINITION  synthetic circular DNAKEYWORDS    pENTR-GUSSOURCE      synthetic DNA construct  ORGANISM  synthetic DNA constructREFERENCE   1  (bases 1 to 3841)  AUTHORS   Biofeng Lab.FEATURES             Location/Qualifiers     source          1..3841                     /organism="synthetic DNA construct"                     /mol_type="other DNA"     protein_bind    99..198                     /gene="mutant version of attL"                     /bound_moiety="LR Clonase(TM)"                     /note="attL1"                     /note="recombination site for the Gateway(R) LR reaction"     CDS             228..2039                     /codon_start=1                     /gene="uidA (or gusA) from E. coli"                     /product="beta-glucuronidase"                     /note="GUS"                     /translation="MVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"     protein_bind    complement(2041..2140)                     /gene="mutant version of attL"                     /bound_moiety="LR Clonase(TM)"                     /note="attL2"                     /note="recombination site for the Gateway(R) LR reaction"     rep_origin      complement(2259..2847)                     /direction=LEFT                     /note="ori"                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of                      replication"     CDS             complement(2990..3805)                     /codon_start=1                     /gene="aph(3')-Ia"                     /product="aminoglycoside phosphotransferase"                     /note="KanR"                     /note="confers resistance to kanamycin in bacteria or G418                      (Geneticin(R)) in eukaryotes"                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"ORIGIN        1 ttttattgtt catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga       61 cacgggccag agctgcagct agcatggatc tcgggcccca aataatgatt ttattttgac      121 tgatagtgac ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt      181 tgtacaaaaa agcaggctca tttaacttta agaaggagat atataccatg gtccgtcctg      241 tagaaacccc aacccgtgaa atcaaaaaac tcgacggcct gtgggcattc agtctggatc      301 gcgaaaactg tggaattgat cagcgttggt gggaaagcgc gttacaagaa agccgggcaa      361 ttgctgtgcc aggcagtttt aacgatcagt tcgccgatgc agatattcgt aattatgcgg      421 gcaacgtctg gtatcagcgc gaagtcttta taccgaaagg ttgggcaggc cagcgtatcg      481 tgctgcgttt cgatgcggtc actcattacg gcaaagtgtg ggtcaataat caggaagtga      541 tggagcatca gggcggctat acgccatttg aagccgatgt cacgccgtat gttattgccg      601 ggaaaagtgt acgtatcacc gtttgtgtga acaacgaact gaactggcag actatcccgc      661 cgggaatggt gattaccgac gaaaacggca agaaaaagca gtcttacttc catgatttct      721 ttaactatgc cggaatccat cgcagcgtaa tgctctacac cacgccgaac acctgggtgg      781 acgatatcac cgtggtgacg catgtcgcgc aagactgtaa ccacgcgtct gttgactggc      841 aggtggtggc caatggtgat gtcagcgttg aactgcgtga tgcggatcaa caggtggttg      901 caactggaca aggcactagc gggactttgc aagtggtgaa tccgcacctc tggcaaccgg      961 gtgaaggtta tctctatgaa ctgtgcgtca cagccaaaag ccagacagag tgtgatatct     1021 acccgcttcg cgtcggcatc cggtcagtgg cagtgaaggg cgaacagttc ctgattaacc     1081 acaaaccgtt ctactttact ggctttggtc gtcatgaaga tgcggactta cgtggcaaag     1141 gattcgataa cgtgctgatg gtgcacgacc acgcattaat ggactggatt ggggccaact     1201 cctaccgtac ctcgcattac ccttacgctg aagagatgct cgactgggca gatgaacatg     1261 gcatcgtggt gattgatgaa actgctgctg tcggctttaa cctctcttta ggcattggtt     1321 tcgaagcggg caacaagccg aaagaactgt acagcgaaga ggcagtcaac ggggaaactc     1381 agcaagcgca cttacaggcg attaaagagc tgatagcgcg tgacaaaaac cacccaagcg     1441 tggtgatgtg gagtattgcc aacgaaccgg atacccgtcc gcaaggtgca cgggaatatt     1501 tcgcgccact ggcggaagca acgcgtaaac tcgacccgac gcgtccgatc acctgcgtca     1561 atgtaatgtt ctgcgacgct cacaccgata ccatcagcga tctctttgat gtgctgtgcc     1621 tgaaccgtta ttacggatgg tatgtccaaa gcggcgattt ggaaacggca gagaaggtac     1681 tggaaaaaga acttctggcc tggcaggaga aactgcatca gccgattatc atcaccgaat     1741 acggcgtgga tacgttagcc gggctgcact caatgtacac cgacatgtgg agtgaagagt     1801 atcagtgtgc atggctggat atgtatcacc gcgtctttga tcgcgtcagc gccgtcgtcg     1861 gtgaacaggt atggaatttc gccgattttg cgacctcgca aggcatattg cgcgttggcg     1921 gtaacaagaa agggatcttc actcgcgacc gcaaaccgaa gtcggcggct tttctgctgc     1981 aaaaacgctg gactggcatg aacttcggtg aaaaaccgca gcagggaggc aaacaataat     2041 acccagcttt cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca     2101 acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct agcggtaata     2161 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa     2221 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct     2281 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa     2341 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg     2401 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca     2461 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa     2521 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg     2581 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg     2641 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga     2701 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc     2761 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag     2821 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac     2881 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagcttgcg ccgtcccgtc     2941 aagtcagcgt aatgctctgc cagtgttaca accaattaac caattctgat tagaaaaact     3001 catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt     3061 gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa     3121 gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc     3181 cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg     3241 agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct     3301 cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga     3361 gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc     3421 gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata     3481 cctggaatgc tgtttttccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac     3541 ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca     3601 tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg     3661 catcgggctt cccatacaag cgatagattg tcgcacctga ttgcccgaca ttatcgcgag     3721 cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctcgagcaag     3781 acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca     3841 g