RNAi-Ready pSIREN-RetroQ-ZsGreen1载体

LOCUS     pSIREN-RetroQ-ZsGreen1_linear   6561 bp    DNA   circular                    12-NOV-2009COMMENT     Created by Clontech Laboratories Inc. COMMENT     Cat. No. 632455COMMENT     This file is created by Vector NTI            http://www.biofeng.com/COMMENT     VNTAUTHORNAME|biofeng.com|FEATURES             Location/Qualifiers     misc_feature    4728..5454                     /label=5'-LTR\(CMV/MSV)     misc_feature    5484..6293                     /label=Extended\packaging\signal     misc_feature    1433..1858                     /label=3'\MMLV\LTR\(deletion\in\U3)     CDS             complement(3505..4365)                     /label=Amp(R)     misc_feature    2138..2424                     /label=SV40\Promoter     promoter        6309..6561                     /label=Human\U6\promoter     rep_origin      complement(2744..3343)                     /label=pUC\origin     rep_origin      2359..2424                     /label=SV40\origin     promoter        6..594                     /label=CMV\IE\Promoter     CDS             618..1313                     /label=ZsGreen1     misc_feature    6561..1                     /label=shRNA\Cloning\SiteBASE COUNT     1572 a      1756 c      1626 g      1607 t ORIGIN        1 aattctagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag        61 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc       121 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga       181 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat       241 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc       301 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct       361 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca       421 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat       481 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg       541 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca gatccgctag       601 cgctaccggt cgccaccatg gcccagtcca agcacggcct gaccaaggag atgaccatga       661 agtaccgcat ggagggctgc gtggacggcc acaagttcgt gatcaccggc gagggcatcg       721 gctacccctt caagggcaag caggccatca acctgtgcgt ggtggagggc ggccccttgc       781 ccttcgccga ggacatcttg tccgccgcct tcatgtacgg caaccgcgtg ttcaccgagt       841 acccccagga catcgtcgac tacttcaaga actcctgccc cgccggctac acctgggacc       901 gctccttcct gttcgaggac ggcgccgtgt gcatctgcaa cgccgacatc accgtgagcg       961 tggaggagaa ctgcatgtac cacgagtcca agttctacgg cgtgaacttc cccgccgacg      1021 gccccgtgat gaagaagatg accgacaact gggagccctc ctgcgagaag atcatccccg      1081 tgcccaagca gggcatcttg aagggcgacg tgagcatgta cctgctgctg aaggacggtg      1141 gccgcttgcg ctgccagttc gacaccgtgt acaaggccaa gtccgtgccc cgcaagatgc      1201 ccgactggca cttcatccag cacaagctga cccgcgagga ccgcagcgac gccaagaacc      1261 agaagtggca cctgaccgag cacgccatcg cctccggctc cgccttgccc taactcgaga      1321 tatcagtggt ccaggctcta gttttgactc aacaatatca ccagctgaag cctatagagt      1381 acgagccata gataaaataa aagattttat ttagtctcca gaaaaagggg ggaatgaaag      1441 accccacctg taggtttggc aagctagctt aagtaacgcc attttgcaag gcatggaaaa      1501 atacataact gagaatagag aagttcagat caaggtcagg aacagatgga acagggtcga      1561 ccctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct      1621 tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag      1681 ctcaataaaa gagcccacaa cccctcactc ggggcgccag tcctccgatt gactgagtcg      1741 cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct      1801 gttccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcatttg      1861 ggggctcgtc cgggatcggg agacccctgc ccagggacca ccgacccacc accgggaggt      1921 aagctggctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc      1981 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg      2041 cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg      2101 gagtgtagat ccggctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc      2161 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt      2221 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca      2281 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc      2341 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg      2401 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctta      2461 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga      2521 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct      2581 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc      2641 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg      2701 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg      2761 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg      2821 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac      2881 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca      2941 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt      3001 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc      3061 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag      3121 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac      3181 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt      3241 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa      3301 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg      3361 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa      3421 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat      3481 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc      3541 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat      3601 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc      3661 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc      3721 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag      3781 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg      3841 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg      3901 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag      3961 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt      4021 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga      4081 atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc      4141 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc      4201 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc      4261 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc      4321 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca      4381 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat      4441 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt      4501 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt      4561 tcgtcttcaa gaattagctt ggccattgca tacgttgtat ccatatcata atatgtacat      4621 ttatattggc tcatgtccaa cattaccgcc atgttgacat tgattattga ctagttatta      4681 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata      4741 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat      4801 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga      4861 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc      4921 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt      4981 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat      5041 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag      5101 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc      5161 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga      5221 ggtctatata agcagagctc aataaaagag cccacaaccc ctcactcggc gcgccagtct      5281 tccgatagac tgcgtcgccc gggtacccgt attcccaata aagcctcttg ctgtttgcat      5341 ccgaatcgtg gtctcgctgt tccttgggag ggtctcctct gagtgattga ctacccacga      5401 cgggggtctt tcatttgggg gctcgtccgg gatttggaga cccctgccca gggaccaccg      5461 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg      5521 tctatgtttg atgttatgcg cctgcgtctg tactagttag ctaactagct ctgtatctgg      5581 cggacccgtg gtggaactga cgagttctga acacccggcc gcaaccctgg gagacgtccc      5641 agggactttg ggggccgttt ttgtggcccg acctgaggaa gggagtcgat gtggaatccg      5701 accccgtcag gatatgtggt tctggtagga gacgagaacc taaaacagtt cccgcctccg      5761 tctgaatttt tgctttcggt ttggaaccga agccgcgcgt cttgtctgct gcagcgctgc      5821 agcatcgttc tgtgttgtct ctgtctgact gtgtttctgt atttgtctga aaattagggc      5881 cagactgtta ccactccctt aagtttgacc ttaggtcact ggaaagatgt cgagcggatc      5941 gctcacaacc agtcggtaga tgtcaagaag agacgttggg ttaccttctg ctctgcagaa      6001 tggccaacct ttaacgtcgg atggccgcga gacggcacct ttaaccgaga cctcatcacc      6061 caggttaaga tcaaggtctt ttcacctggc ccgcatggac acccagacca ggtcccctac      6121 atcgtgacct gggaagcctt ggcttttgac ccccctccct gggtcaagcc ctttgtacac      6181 cctaagcctc cgcctcctct tcctccatcc gccccgtctc tcccccttga acctcctcgt      6241 tcgaccccgc ctcgatcctc cctttatcca gccctcactc cttctctagg cgccggaatt      6301 gaagatctgg gcaggaagag ggcctatttc ccatgattcc ttcatatttg catatacgat      6361 acaaggctgt tagagagata attagaatta atttgactgt aaacacaaag atattagtac      6421 aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt      6481 ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat      6541 atatcttgtg gaaaggacga g //