pRetroX-PTuner2载体

LOCUS       pRetroX-PTuner2   7345 bp    DNA   circular            11-APR-2011COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.comCOMMENT     Cat. No. 631097,631093FEATURES             Location/Qualifiers     misc_feature    1..727                     /label=CMV/MSV\5'\LTR     misc_feature    757..1566                     /label=Psi\-\Extended\packaging\signal     misc_feature    4044..4476                     /label=3'\MMLV\LTR\(deletion\in\U3)     CDS             complement(6123..6983)                     /label=Amp(R)     promoter        2731..3239                     /label=PGK\Promoter     CDS             3260..3859                     /label=Puro(R)     polyA_signal    4302..4317                     /label=PolyA\signal     rep_origin      4977..5042                     /label=SV40\origin     promoter        1582..2170                     /label=CMV\IE\Promoter     promoter        4756..5023                     /label=SV40\Promoter     rep_origin      5363..5363                     /label=pUC\origin     CDS             2178..2654                     /label=DD-GBASE COUNT     1661 a      2059 c      1910 g      1715 t ORIGIN        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc        61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac       121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata       181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc       241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta       301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac       361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc       421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc       481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc       541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc       601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat       661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc       721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc       781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta       841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc       901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc       961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca      1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct      1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc      1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga      1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt      1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg      1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga      1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa      1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct      1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct      1561 aggcgccgga attgaagatc atagttatta atagtaatca attacggggt cattagttca      1621 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc      1681 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat      1741 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt      1801 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc      1861 cgccttgcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta      1921 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg      1981 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt      2041 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac      2101 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa      2161 ccgtcagatc cgctagcatg atcagtctga ttgcggcgtt agcggtagat ctcgttatcg      2221 gcatggaaaa cgccatgccg tggaacctgc ctgccgatct cgcctggttt aaacgcaaca      2281 ccttaaataa acccgtgatt atgggccgcc atacctggga atcaatcggt cgtccgttgc      2341 caggacgcaa aaatattatc ctcagcagtc aaccgagtac ggacgatcgc gtaacgtggg      2401 tgaagtcggt ggatgaagcc atcgcggcgt gtggtgacgt accagaaatc atggtgattg      2461 gcggcggtcg cgttattgaa cagttcttgc caaaagcgca aaaactgtat ctgacgcata      2521 tcgacgcaga agtggaaggc gacactcatt tcccggatta cgagccggat gactgggaat      2581 cggtattcag cgaattccac gatgctgatg cgcagaactc tcacagctat tgctttgaga      2641 ttctggagcg gcgactcgag ctcaagcttc gaattctgca gtcgacggta ccgcgggccc      2701 gggatccacc ggatctagat aactgatcat aattctaccg ggtaggggag gcgcttttcc      2761 caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg      2821 gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg      2881 gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc      2941 agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga      3001 tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc      3061 tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca      3121 ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca      3181 cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga      3241 cctgcagccc aagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga      3301 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg      3361 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct      3421 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc      3481 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg      3541 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc      3601 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca      3661 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc      3721 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct      3781 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac      3841 ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc      3901 acgaccccat gcatcggcgt ctcgatcgag atatcagtgg tccaggctct agttttgact      3961 caacaatatc accagctgaa gcctatagag tacgagccat agataaaata aaagatttta      4021 tttagtctcc agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctacta      4081 gcttaagtaa cgccattttg caaggcatgg aaaaatacat aactgagaat agagaagttc      4141 agatcaaggt caggaacaga tggaacaggg tcgatcgacc ctagagaacc atcagatgtt      4201 tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt      4261 cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct caataaaaga gcccacaacc      4321 cctcactcgg ggcgccagtc ctccgattga ctgagtcgcc cgggtacccg tgtatccaat      4381 aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt tccttgggag ggtctcctct      4441 gagtgattga ctacccgtca gcgggggtct ttcatttggg ggctcgtccg ggatcgggag      4501 acccctgccc agggaccacc gacccaccac cgggaggtaa gctggctgcc tcgcgcgttt      4561 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct      4621 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg      4681 tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtagatcc ggctgtggaa      4741 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag      4801 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag      4861 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc      4921 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt      4981 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg      5041 aggctttttt ggaggcctag gcttttgcaa aaagcttact ggcttaacta tgcggcatca      5101 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg      5161 agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc      5221 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa      5281 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt      5341 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa