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保存条件 :常温
保质期 :三年
英文名 :pCDF1-MCS2-EF1-copGFP
库存 :60
供应商 :信裕生物
规格 :5ug质粒
pCDF1-MCS2-EF1-copGFP载体基本信息
质粒类型: | 慢病毒载体 |
---|---|
启动子: | CMV |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 6771 bp (查看载体序列) |
5' 测序引物及序列: | LNCX: AGCTCGTTTAGTGAACCGTCAGATC |
3' 测序引物及序列: | EF1a-R: TTTCGCCCTAACTTCGTGAT |
载体标签: | copGFP |
载体抗性: | Ampicillin (氨苄青霉素) |
订购信息
产品编号 | 产品名称 | 规格 | 价格 |
---|---|---|---|
XY1483 | pCDF1-MCS2-EF1-copGFP | 5ug质粒 |
¥1000.00 |
质粒图谱
The pCDF1-MCS2-EF1-copGFP Vector contains the full-length copGFP gene with optimized human codons for high level of expression of the fluorescent protein from the CMV promoter in mammalian cells. The copGFP marker is a novel natural green monomeric GFP-like protein from copepod (Pontellina sp.). The copGFP protein is a non-toxic, non-aggregating protein with fast protein maturation, high stability at a wide range of pH (pH 4-12), and does not require any additional cofactors or substrates. The copGFP protein has very bright fluorescence that exceeds at least 1.3 times the brightness of EGFP, the widely used Aequorea victoria GFP mutant. The copGFP protein emits green fluorescence with the following characteristics:
emission wavelength max – 502 nm;
excitation wavelength max – 482 nm;
quantum yield – 0.6;
extinction coefficient – 70,000 M-1 cm-1
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