pMSCV-FLIP-puro-dsRed-GFP-miRNA载体

LOCUS       pMSCV-FLIP-puro-dsRed-GFP-miRNA	8376 bp 	DNA	SYNDEFINITION  pMSCV-FLIP-puro-dsRed-GFP-miRNAACCESSION   KEYWORDS    SOURCE        ORGANISM  other sequences; artificial sequences; vectors.FEATURES             Location/Qualifiers     source          1..8376                     /organism="pMSCV-FLIP-puro-dsRed-GFP-miRNA"                     /mol_type="other DNA"     misc_feature    655..1404                     /label="psi_plus_pack"     misc_feature    991..1398                     /label="gag"     misc_feature    1334..1356                     /label="pLXSN_5_primer"     misc_feature    1334..1356                     /label="MSCV_primer"     misc_feature    1372..1388                     /label="pBABE_5_primer"     misc_feature    complement(1417..1450)                     /label="loxP"     misc_feature    1417..1450                     /label="loxP"     CDS             1467..2852                     /label="ORF frame 3"                     /translation="MVEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL                     ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGALYKKQKIVAPVKQTLNFDL                     LKLAGDVESNPGPGPVATMDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEG                     TQTAKLQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMN                     FEDGGVVTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGV                     LKGEIHKALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQY                     ERAEARHHLFQ*"     gene            1479..2054                     /label="puro (variant)"                     /gene="puro (variant)"                     /translation="MVEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL                     ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGALYKKQKIVAPVKQTLNFDL                     LKLAGDVESNPGPGPVATMDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEG                     TQTAKLQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMN                     FEDGGVVTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGV                     LKGEIHKALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQY                     ERAEARHHLFQ*"     misc_feature    complement(2355..2375)                     /label="dsRed1_N_primer"                     /translation="MVEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL                     ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGALYKKQKIVAPVKQTLNFDL                     LKLAGDVESNPGPGPVATMDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEG                     TQTAKLQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMN                     FEDGGVVTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGV                     LKGEIHKALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQY                     ERAEARHHLFQ*"     misc_feature    2767..2790                     /label="dsRed1_C_primer"                     /translation="MVEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL                     ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGALYKKQKIVAPVKQTLNFDL                     LKLAGDVESNPGPGPVATMDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEG                     TQTAKLQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMN                     FEDGGVVTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGV                     LKGEIHKALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQY                     ERAEARHHLFQ*"     CDS             complement(3152..3871)                     /label="ORF frame 3"                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT                     LKFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKN                     GIKVNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH                     MVLLEFVTAAGITLGMDELYK*"     gene            complement(3155..3868)                     /label="EGFP (variant)"                     /gene="EGFP (variant)"                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT                     LKFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKN                     GIKVNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH                     MVLLEFVTAAGITLGMDELYK*"     misc_feature    complement(3197..3218)                     /label="EGFP_C_primer"                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT                     LKFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKN                     GIKVNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH                     MVLLEFVTAAGITLGMDELYK*"     misc_feature    3805..3826                     /label="EGFP_N_primer"                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT                     LKFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKN                     GIKVNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH                     MVLLEFVTAAGITLGMDELYK*"     misc_feature    3885..3918                     /label="loxP"                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT                     LKFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKN                     GIKVNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH                     MVLLEFVTAAGITLGMDELYK*"     misc_feature    complement(3965..3998)                     /label="loxP"                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT                     LKFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKN                     GIKVNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH                     MVLLEFVTAAGITLGMDELYK*"     misc_feature    3965..3998                     /label="loxP"                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT                     LKFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKN                     GIKVNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH                     MVLLEFVTAAGITLGMDELYK*"     CDS             3978..4655                     /label="ORF frame 3"                     /translation="MCTIRSYPGLNDLQPSLSIIPDNQPLDYKICERLTGILNYVAPF                     TLCGYAALMPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVFADATPT                     GWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGKLTSFPW                     LLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRISLWAASP                     HRELSIK*"     misc_feature    4034..4638                     /label="WPRE"                     /translation="MCTIRSYPGLNDLQPSLSIIPDNQPLDYKICERLTGILNYVAPF                     TLCGYAALMPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVFADATPT                     GWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGKLTSFPW                     LLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRISLWAASP                     HRELSIK*"     CDS             complement(4131..4634)                     /label="ORF frame 2"                     /translation="MRGGGPKGDPTRLRAKAKTRKRPQSRQQAAGRKVRWIEGRRDVA                     EGRPAQNPGGNTGEQPWKGRQLPRQHHGIVSAQQPSPCPAAGKAGGDEFRRGNREGES                     ESPGKELTGGGNAPTSGGCVSKHSAHHATLPDNGPQLLIKRQQPGFIQGGENESHTGS                     NSMIQRH*"     promoter        complement(5369..5387)                     /label="M13_reverse_primer"                     /translation="MRGGGPKGDPTRLRAKAKTRKRPQSRQQAAGRKVRWIEGRRDVA                     EGRPAQNPGGNTGEQPWKGRQLPRQHHGIVSAQQPSPCPAAGKAGGDEFRRGNREGES                     ESPGKELTGGGNAPTSGGCVSKHSAHHATLPDNGPQLLIKRQQPGFIQGGENESHTGS                     NSMIQRH*"     misc_feature    complement(5386..5408)                     /label="M13_pUC_rev_primer"                     /translation="MRGGGPKGDPTRLRAKAKTRKRPQSRQQAAGRKVRWIEGRRDVA                     EGRPAQNPGGNTGEQPWKGRQLPRQHHGIVSAQQPSPCPAAGKAGGDEFRRGNREGES                     ESPGKELTGGGNAPTSGGCVSKHSAHHATLPDNGPQLLIKRQQPGFIQGGENESHTGS                     NSMIQRH*"     promoter        complement(5422..5451)                     /label="lac_promoter"                     /translation="MRGGGPKGDPTRLRAKAKTRKRPQSRQQAAGRKVRWIEGRRDVA                     EGRPAQNPGGNTGEQPWKGRQLPRQHHGIVSAQQPSPCPAAGKAGGDEFRRGNREGES                     ESPGKELTGGGNAPTSGGCVSKHSAHHATLPDNGPQLLIKRQQPGFIQGGENESHTGS                     NSMIQRH*"     gene            complement(6534..7394)                     /label="Ampicillin"                     /gene="Ampicillin"                     /translation="MRGGGPKGDPTRLRAKAKTRKRPQSRQQAAGRKVRWIEGRRDVA                     EGRPAQNPGGNTGEQPWKGRQLPRQHHGIVSAQQPSPCPAAGKAGGDEFRRGNREGES                     ESPGKELTGGGNAPTSGGCVSKHSAHHATLPDNGPQLLIKRQQPGFIQGGENESHTGS                     NSMIQRH*"     CDS             complement(6534..7394)                     /label="ORF frame 2"                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA                     EIGASLIKHW*"     promoter        complement(7436..7464)                     /label="AmpR_promoter"                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA                     EIGASLIKHW*"     misc_feature    complement(7623..7645)                     /label="pGEX_3_primer"                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA                     EIGASLIKHW*"     misc_feature    complement(7844..7975)                     /label="lacZ_a"                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA                     EIGASLIKHW*"     misc_feature    7958..7980                     /label="M13_pUC_fwd_primer"                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA                     EIGASLIKHW*"     misc_feature    8156..8175                     /label="pBRrevBam_primer"                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA                     EIGASLIKHW*"ORIGIN    1 TGAAAGACCC CACCTGTAGG TTTGGCAAGC TAGCTTAAGT AACGCCATTT TGCAAGGCAT   61 GGAAAATACA TAACTGAGAA TAGAGAAGTT CAGATCAAGG TTAGGAACAG AGAGACAGCA  121 GAATATGGGC CAAACAGGAT ATCTGTGGTA AGCAGTTCCT GCCCCGGCTC AGGGCCAAGA  181 ACAGATGGTC CCCAGATGCG GTCCCGCCCT CAGCAGTTTC TAGAGAACCA TCAGATGTTT  241 CCAGGGTGCC CCAAGGACCT GAAAATGACC CTGTGCCTTA TTTGAACTAA CCAATCAGTT  301 CGCTTCTCGC TTCTGTTCGC GCGCTTCTGC TCCCCGAGCT CAATAAAAGA GCCCACAACC  361 CCTCACTCGG CGCGCCAGTC CTCCGATAGA CTGCGTCGCC CGGGTACCCG TATTCCCAAT  421 AAAGCCTCTT GCTGTTTGCA TCCGAATCGT GGACTCGCTG ATCCTTGGGA GGGTCTCCTC  481 AGATTGATTG ACTGCCCACC TCGGGGGTCT TTCATTTGGA GGTTCCACCG AGATTTGGAG  541 ACCCCTGCCT AGGGACCACC GACCCCCCCG CCGGGAGGTA AGCTGGCCAG CGGTCGTTTC  601 GTGTCTGTCT CTGTCTTTGT GCGTGTTTGT GCCGGCATCT AATGTTTGCG CCTGCGTCTG  661 TACTAGTTAG CTAACTAGCT CTGTATCTGG CGGACCCGTG GTGGAACTGA CGAGTTCTGA  721 ACACCCGGCC GCAACCCTGG GAGACGTCCC AGGGACTTTG GGGGCCGTTT TTGTGGCCCG  781 ACCTGAGGAA GGGAGTCGAT GTGGAATCCG ACCCCGTCAG GATATGTGGT TCTGGTAGGA  841 GACGAGAACC TAAAACAGTT CCCGCCTCCG TCTGAATTTT TGCTTTCGGT TTGGAACCGA  901 AGCCGCGCGT CTTGTCTGCT GCAGCGCTGC AGCATCGTTC TGTGTTGTCT CTGTCTGACT  961 GTGTTTCTGT ATTTGTCTGA AAATTAGGGC CAGACTGTTA CCACTCCCTT AAGTTTGACC 1021 TTAGGTCACT GGAAAGATGT CGAGCGGATC GCTCACAACC AGTCGGTAGA TGTCAAGAAG 1081 AGACGTTGGG TTACCTTCTG CTCTGCAGAA TGGCCAACCT TTAACGTCGG ATGGCCGCGA 1141 GACGGCACCT TTAACCGAGA CCTCATCACC CAGGTTAAGA TCAAGGTCTT TTCACCTGGC 1201 CCGCATGGAC ACCCAGACCA GGTCCCCTAC ATCGTGACCT GGGAAGCCTT GGCTTTTGAC 1261 CCCCCTCCCT GGGTCAAGCC CTTTGTACAC CCTAAGCCTC CGCCTCCTCT TCCTCCATCC 1321 GCCCCGTCTC TCCCCCTTGA ACCTCCTCGT TCGACCCCGC CTCGATCCTC CCTTTATCCA 1381 GCCCTCACTC CTTCTCTAGG CGCCGGAATT AGAtccataa cttcgtatag gataccttat 1441 acgaagttat ctcaggtacc GCCACCATGG TGGAGTACAA GCCCACGGTG CGCCTCGCCA 1501 CCCGCGACGA CGTCCCCAGG GCCGTACGCA CCCTCGCCGC CGCGTTCGCC GACTACCCCG 1561 CCACGCGCCA CACCGTCGAT CCGGACCGCC ACATCGAGCG GGTCACCGAG CTGCAAGAAC 1621 TCTTCCTCAC GCGCGTCGGG CTCGACATCG GCAAGGTGTG GGTCGCGGAC GACGGCGCCG 1681 CGGTGGCGGT CTGGACCACG CCGGAGAGCG TCGAAGCGGG GGCGGTGTTC GCCGAGATCG 1741 GCCCGCGCAT GGCCGAGTTG AGCGGTTCCC GGCTGGCCGC GCAGCAACAG ATGGAAGGCC 1801 TCCTGGCGCC GCACCGGCCC AAGGAGCCCG CGTGGTTCCT GGCCACCGTC GGCGTCTCGC 1861 CCGACCACCA GGGCAAGGGT CTGGGCAGCG CCGTCGTGCT CCCCGGAGTG GAGGCGGCCG 1921 AGCGCGCCGG GGTGCCCGCC TTCCTGGAGA CCTCCGCGCC CCGCAACCTC CCCTTCTACG 1981 AGCGGCTCGG CTTCACCGTC ACCGCCGACG TCGAGGTGCC CGAAGGACCG CGCACCTGGT 2041 GCATGACCCG CAAGCCCGGT GCCCTGTACA AGAAACAGAA AATTGTGGCA CCAGTGAAAC 2101 AGACTTTGAA TTTTGACCTT CTCAAGTTGG CGGGAGACGT CGAGTCCAAC CCTGGGCCCG 2161 GCCCGGTCGC CACCATGGAT AGCACTGAGA ACGTCATCAA GCCCTTCATG CGCTTCAAGG 2221 TGCACATGGA GGGCTCCGTG AACGGCCACG AGTTCGAGAT CGAGGGCGAG GGCGAGGGCA 2281 AGCCCTACGA GGGCACCCAG ACCGCCAAGC TGCAGGTGAC CAAGGGCGGC CCCCTGCCCT 2341 TCGCCTGGGA CATCCTGTCC CCCCAGTTCC AGTACGGCTC CAAGGTGTAC GTGAAGCACC 2401 CCGCCGACAT CCCCGACTAC AAGAAGCTGT CCTTCCCCGA GGGCTTCAAG TGGGAGCGCG 2461 TGATGAACTT CGAGGACGGC GGCGTGGTGA CCGTGACCCA GGACTCCTCC CTGCAGGACG 2521 GCACCTTCAT CTACCACGTG AAGTTCATCG GCGTGAACTT CCCCTCCGAC GGCCCCGTAA 2581 TGCAGAAGAA GACTCTGGGC TGGGAGCCCT CCACCGAGCG CCTGTACCCC CGCGACGGCG 2641 TGCTGAAGGG CGAGATCCAC AAGGCGCTGA AGCTGAAGGG CGGCGGCCAC TACCTGGTGG 2701 AGTTCAAGTC AATCTACATG GCCAAGAAGC CCGTGAAGCT GCCCGGCTAC TACTACGTGG 2761 ACTCCAAGCT GGACATCACC TCCCACAACG AGGACTACAC CGTGGTGGAG CAGTACGAGC 2821 GCGCCGAGGC CCGCCACCAC CTGTTCCAGT AGtctagaag ccataacttc gtatagtaca 2881 cattatacga aGTTATGCAT ACCTGGAGGA AAAAAAAAAG GGGAGAGTCA GGGGTTTAAA 2941 CTCGAGTGCG GCCAGATCTG GGCCATTTGT TCCATGTGAG TGCTAGTAAC AGGCCTTGTG 3001 TCCTGACAAC AGGCAGCATA ATGAGTCAGT CAGTGGCCAA AACTCATTAT GGACTGCCTG 3061 TTGTCAGCAT ACAGCCTTCA GCAAGCCTCC AGGATCCACT GGTCGACTCA CTACCTCCCT 3121 TTAAACGATC GACGGCCACG AAGTGCTTAG CTTACTTGTA CAGCTCGTCC ATGCCGAGAG 3181 TGATCCCGGC GGCGGTCACG AACTCCAGCA GGACCATGTG ATCGCGCTTC TCGTTGGGGT 3241 CTTTGCTCAG GGCGGACTGG GTGCTCAGGT AGTGGTTGTC GGGCAGCAGC ACGGGGCCGT 3301 CGCCGATGGG GGTGTTCTGC TGGTAGTGGT CGGCGAGCTG CACGCTGCCG TCCTCGATGT 3361 TGTGGCGGGT CTTGAAGTTC ACCTTGATGC CGTTCTTCTG CTTGTCGGCG GTGATATAGA 3421 CCTTGTGGCT GTTGTAGTTG TACTCCAGCT TGTGCCCCAG GATGTTGCCG TCCTCCTTGA 3481 AGTCGATGCC CTTCAGCTCG ATGCGGTTCA CCAGGGTGTC GCCCTCGAAC TTCACCTCGG 3541 CGCGGGTCTT GTAGTTGCCG TCGTCCTTGA AGAAGATGGT GCGCTCCTGG ACGTAGCCTT 3601 CGGGCATGGC GGACTTGAAG AAGTCGTGCT GCTTCATGTG GTCGGGGTAG CGGGCGAAGC 3661 ACTGCACGCC GTAGGTGAAG GTGGTCACGA GGGTGGGCCA GGGCACGGGC AGCTTGCCGG 3721 TGGTGCAGAT GAACTTCAGG GTCAGCTTGC CGTAGGTGGC ATCGCCCTCG CCCTCGCCGG 3781 ACACGCTGAA CTTGTGGCCG TTTACGTCGC CGTCCAGCTC GACCAGGATG GGCACCACCC 3841 CGGTGAACAG CTCCTCGCCC TTGCTCACCA TGGTGGCGac cggtataact tcgtataagg 3901 tatcctatac gaagttatcc attcaggctg tgctagcatc aatggcatgg cacaaagctt 3961 agccataact tcgtataatg tgtactatac gaagttatcc cgggttAAAC GACCTGCAGC 4021 CAAGCTTATC GATaattccc gataatcaac ctctggatta caaaatttgt gaaagattga 4081 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt 4141 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt 4201 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg 4261 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg 4321 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc 4381 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc 4441 tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct 4501 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg 4561 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg 4621 ccgcctcccc gcatcgggaa ttATCGATAA AATAAAAGAT TTTATTTAGT CTCCAGAAAA 4681 AGGGGGGAAT GAAAGACCCC ACCTGTAGGT TTGGCAAGCT AGCTTAAGTA ACGCCATTTT 4741 GCAAGGCATG GAAAATACAT AACTGAGAAT AGAGAAGTTC AGATCAAGGT TAGGAACAGA 4801 GAGACAGCAG AATATGGGCC AAACAGGATA TCTGTGGTAA GCAGTTCCTG CCCCGGCTCA 4861 GGGCCAAGAA CAGATGGTCC CCAGATGCGG TCCCGCCCTC AGCAGTTTCT AGAGAACCAT 4921 CAGATGTTTC CAGGGTGCCC CAAGGACCTG AAAATGACCC TGTGCCTTAT TTGAACTAAC 4981 CAATCAGTTC GCTTCTCGCT TCTGTTCGCG CGCTTCTGCT CCCCGAGCTC AATAAAAGAG 5041 CCCACAACCC CTCACTCGGC GCGCCAGTCC TCCGATAGAC TGCGTCGCCC GGGTACCCGT 5101 GTATCCAATA AACCCTCTTG CAGTTGCATC CGACTTGTGG TCTCGCTGTT CCTTGGGAGG 5161 GTCTCCTCTG AGTGATTGAC TACCCGTCAG CGGGGGTCTT TCATGGGTAA CAGTTTCTTG 5221 AAGTTGGAGA ACAACATTCT GAGGGTAGGA GTCGAATATT AAGTAATCCT GACTCAATTA 5281 GCCACTGTTT TGAATCCACA TACTCCAATA CTCCTGAAAT AGTTCATTAT GGACAGCGCA 5341 GAAGAGCTGG GGAGAATTAA TTCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT 5401 TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT 5461 GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG 5521 GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG 5581 CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG 5641 CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT 5701 AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC 5761 GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC