pLVX-CherryPicker Control载体

LOCUS       pLVX-CherryPicker_Control   8999 bp    DNA   circular            11-APR-2011COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.comCOMMENT     Cat. No. 632580,632574,632581,632575FEATURES             Location/Qualifiers     LTR             1..635                     /label=5'\LTR     misc_feature    685..822                     /label=Packaging\signal     misc_feature    2028..2151                     /label=cPPT/CTS\(DNA\flap)     promoter        2185..2787                     /label=CMV\IE\Promoter     misc_feature    4967..5558                     /label=WPRE     rep_origin      complement(6867..7540)                     /label=pUC\origin     CDS             complement(7685..8681)                     /label=Amp(R)     misc_binding    1303..1536                     /label=RRE     misc_binding    636..653                     /label=PBS     LTR             5761..6397                     /label=3'\LTR     misc_feature    3825..4333                     /label=PGK\Promoter     misc_feature    4354..4953                     /label=Puro(R)     CDS             2885..3811                     /label=CherryPicker     misc_feature    3812..3814                     /label=Stop\codonBASE COUNT     2264 a      2310 c      2406 g      2019 t ORIGIN        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca        61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac       121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca       181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg       241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag       301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg       361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat       421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga       481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct       541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc       601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag       661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg       721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga       781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg       841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg       901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct       961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat      1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca      1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc      1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag      1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc      1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg      1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc      1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc      1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct      1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa      1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca      1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt      1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt      1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta      1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt      1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct      1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga      1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag      2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac      2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg      2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa      2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg      2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt      2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg      2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc      2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc      2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca      2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta      2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa      2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc      2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag      2821 ctcaagcttc gaattctgca gtcgacggta ccgcgggccc gggatccgcg gccgcgttta      2881 aacgatgatg gtggacggcg acaacagcca cgtggagatg aagctggccg tggacgagga      2941 agagaacgcc gacaacaaca ccaaggccaa cgtgaccaag cccaagcggt gcagcggcag      3001 catctgctac ggcacaatcg ccgtgatcgt gttcttcctg atcggcttca tgatcggcta      3061 cctgggctac tgcaagtcta gcgacggccc tggcgagaca ggcatggtgt ccaagggcga      3121 agaggacaac atggccatca tcaaagaatt catgcggttc aaggtgcaca tggaaggcag      3181 cgtgaacggc cacgagttcg agatcgaggg cgagggcgaa ggcagacctt acgagggcac      3241 ccagaccgcc aagctgaaag tgaccaaggg cggacccctg ccctttgcct gggacatcct      3301 gagcccccag ttcatgtacg gcagcaaggc ctacgtgaag caccccgccg acatccccga      3361 ctacctgaag ctgagcttcc ccgagggctt caagtgggag agagtgatga acttcgagga      3421 cggcggcgtg gtcaccgtga cccaggacag cagcctgcag gacggcgagt tcatctacaa      3481 agtgaagctg cggggcacca acttccctag cgacggcccc gtgatgcaga aaaagaccat      3541 gggctgggag gccagcagcg agagaatgta ccccgaggat ggcgccctga agggcgagat      3601 caagcagcgg ctgaagctga aggatggcgg ccactacgac gccgaagtga aaaccaccta      3661 caaggccaag aaacccgtgc agctgcctgg cgcctacaac gtgaacatca agctggacat      3721 caccagccac aacgaggact acaccatcgt ggagcagtac gagagagccg agggcagaca      3781 cagcaccggc ggcatggacg agctgtacaa atgacaatct agataattct accgggtagg      3841 ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg      3901 gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc      3961 ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt      4021 cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac      4081 tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag      4141 cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc      4201 cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg      4261 cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc      4321 tccgggcctt tcgacctgca gcccaagctt accatgaccg agtacaagcc cacggtgcgc      4381 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac      4441 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg      4501 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac      4561 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc      4621 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg      4681 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc      4741 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag      4801 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc      4861 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc      4921 acctggtgca tgacccgcaa gcccggtgcc tgaccgcgtc tggaacaatc aacctctgga      4981 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg      5041 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt      5101 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag      5161 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc      5221 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga      5281 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa      5341 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac      5401 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct      5461 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca      5521 gacgagtcgg atctcccttt gggccgcctc cccgcctgga attaattctg cagtcgagac      5581 ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc      5641 tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta      5701 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagagggga      5761 ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac      5821 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca      5881 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc      5941 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg      6001 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga      6061 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc      6121 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga      6181 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg      6241 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc      6301 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct      6361 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat      6421 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcctt gacattgcta      6481 gcgttttacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg      6541 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta      6601 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg      6661 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga      6721 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg      6781 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag      6841 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc      6901 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca      6961 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt      7021 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc      7081 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc      7141 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc      7201 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact      7261 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg      7321 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta      7381 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca      7441 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa      7501 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg      7561 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc      7621 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg      7681 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat      7741 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg      7801 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa      7861 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca      7921 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc      7981 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt      8041 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa      8101 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat      8161 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct      8221 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga      8281 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag      8341 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga      8401 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca      8461 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg      8521 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc      8581 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag      8641 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatcaac      8701 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat      8761 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat      8821 catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca      8881 taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct      8941 gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagt