pLVX-DD-AmCyan1 Reporte载体

pLVX-DD-AmCyan1 Reporter is a promoterless reporter vector that allows the functional analysis of different promotersand promoter/enhancer combinations inserted into its multiple cloning site (MCS). The vector encodes the reporter proteinDD-AmCyan1, a ligand-dependent, destabilized cyan fluorescent protein that minimizes background fluorescence fromleaky promoters. A promoter must be cloned into the MCS, located upstream of the DD-AmCyan1 coding sequence.Without the addition of a functional promoter, the vector will not express DD-AmCyan1.Location of Features5' LTR: 1–635PBS (primer binding site): 636–653Ψ (packaging signal): 685–822RRE (Rev-response element): 1303–1536cPPT/CTS (central polypurine tract/central termination sequence): 2028–2151MCS (multiple cloning site): 2195–2234DD (FKBP-L106P destabilization domain): 2247–2570AmCyan1 (Anemonia majano cyan fluorescent protein): 2577–3263PPGK (phosphoglycerate kinase promoter): 3274–3782Puror(puromycin resistance gene): 3803–4402WPRE (woodchuck hepatitis virus posttranscriptional regulatory element): 4416–50073' LTR: 5211–5847pUC origin of replication: 6317–6987 (complementary)Ampr(ampicillin resistance gene; β-lactamase): 7132–8128 (complementary)