pLVX-ZsGreen1-C1载体

LOCUS       pLVX-ZsGreen1-C1   8763 bp    DNA   circular             7-OCT-2009COMMENT     Created by Clontech Laboratories Inc.COMMENT     Cat. No. 632566COMMENT     This file is created by Vector NTI            http://www.biofeng.com/COMMENT     VNTAUTHORNAME|biofeng.com|FEATURES             Location/Qualifiers     LTR             1..635                     /label=5'\LTR     misc_feature    685..822                     /label=packaging\signal     misc_feature    4731..5322                     /label=WPRE     CDS             complement(7449..8445)                     /label=Amp(R)     misc_binding    1303..1536                     /label=RRE     misc_binding    636..653                     /label=PBS     LTR             5525..6161                     /label=3'\LTR     promoter        3589..4097                     /label=PGK\Promoter     CDS             4118..4717                     /label=Puro(R)     misc_feature    3574..3576                     /label=Stop     misc_feature    3578..3580                     /label=Stop     CDS             2807..3499                     /label=ZsGreen1     misc_feature    2028..2151                     /label=cPPT/CTS\(DNA\flap)     promoter        2185..2787                     /label=CMV\IE\Promoter     rep_origin      complement(6631..7304)                     /label=pUC\ori     misc_feature    3582..3584                     /label=Stop     misc_feature    3513..3565                     /label=MCSBASE COUNT     2162 a      2283 c      2311 g      2007 t ORIGIN        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca        61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac       121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca       181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg       241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag       301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg       361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat       421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga       481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct       541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc       601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag       661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg       721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga       781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg       841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg       901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct       961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat      1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca      1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc      1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag      1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc      1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg      1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc      1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc      1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct      1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa      1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca      1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt      1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt      1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta      1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt      1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct      1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga      1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag      2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac      2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg      2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa      2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg      2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt      2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg      2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc      2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc      2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca      2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta      2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa      2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc      2761 tccatagaag acaccgactc tactagagga tctaccggtc gccaccatgg cccagtccaa      2821 gcacggcctg accaaggaga tgaccatgaa gtaccgcatg gagggctgcg tggacggcca      2881 caagttcgtg atcaccggcg agggcatcgg ctaccccttc aagggcaagc aggccatcaa      2941 cctgtgcgtg gtggagggcg gccccttgcc cttcgccgag gacatcttgt ccgccgcctt      3001 catgtacggc aaccgcgtgt tcaccgagta cccccaggac atcgtcgact acttcaagaa      3061 ctcctgcccc gccggctaca cctgggaccg ctccttcctg ttcgaggacg gcgccgtgtg      3121 catctgcaac gccgacatca ccgtgagcgt ggaggagaac tgcatgtacc acgagtccaa      3181 gttctacggc gtgaacttcc ccgccgacgg ccccgtgatg aagaagatga ccgacaactg      3241 ggagccctcc tgcgagaaga tcatccccgt gcccaagcag ggcatcttga agggcgacgt      3301 gagcatgtac ctgctgctga aggacggtgg ccgcttgcgc tgccagttcg acaccgtgta      3361 caaggccaag tccgtgcccc gcaagatgcc cgactggcac ttcatccagc acaagctgac      3421 ccgcgaggac cgcagcgacg ccaagaacca gaagtggcac ctgaccgagc acgccatcgc      3481 ctccggctcc gccttgccct ccggactcag atctcgagct caagcttcga attctgcagt      3541 cgacggtacc gcgggcccgg gatccaccgg atctagataa ctgatcataa ttctaccggg      3601 taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca      3661 cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc      3721 aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga      3781 agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc      3841 tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg      3901 gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg      3961 ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg      4021 gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct      4081 catctccggg cctttcgacc tgcagcccaa gcttaccatg accgagtaca agcccacggt      4141 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc      4201 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga      4261 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga      4321 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt      4381 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca      4441 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt      4501 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt      4561 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct      4621 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc      4681 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaccg cgtctggaac aatcaacctc      4741 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc      4801 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca      4861 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg      4921 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca      4981 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg      5041 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg      5101 acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg      5161 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg      5221 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc      5281 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattaat tctgcagtcg      5341 agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg      5401 tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc      5461 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaagag      5521 gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta      5581 ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata      5641 tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga      5701 ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga      5761 cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc      5821 ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact      5881 ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct      5941 cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga      6001 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct      6061 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat      6121 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta      6181 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg ccttgacatt      6241 gctagcgttt taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt      6301 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag      6361 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg      6421 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg      6481 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc      6541 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc      6601 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg      6661 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat      6721 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag      6781 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga      6841 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg      6901 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt      6961 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac      7021 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc      7081 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt      7141 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc      7201 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc      7261 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg      7321 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag      7381 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg      7441 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt      7501 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca      7561 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca      7621 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc      7681 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt      7741 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg      7801 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc      7861 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg      7921 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga      7981 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga      8041 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta      8101 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg      8161 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact      8221 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata      8281 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt      8341 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa      8401 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat      8461 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac      8521 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc      8581 ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac      8641 cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc      8701 ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt      8761 agt