FUW-tetO-hOCT4载体

LOCUS       FUW-tetO-hOCT4	9477 bp 	DNA	SYNDEFINITION  FUW-tetO-hOCT4ACCESSION   KEYWORDS    SOURCE        ORGANISM  other sequences; artificial sequences; vectors.FEATURES             Location/Qualifiers     source          1..9477                     /organism="FUW-tetO-hOCT4"                     /mol_type="other DNA"     promoter        28..604                     /label="CMV_immearly_promoter"     misc_feature    107..394                     /label="CAG_enhancer"     misc_feature    561..581                     /label="CMV_fwd_primer"     misc_feature    624..804                     /label="truncHIV-1_3_LTR"     misc_feature    624..804                     /label="HIV-1_5_LTR"     misc_feature    915..959                     /label="HIV-1_psi_pack"     CDS             1347..2234                     /label="ORF frame 3"     misc_feature    1469..1702                     /label="RRE"     misc_feature    2233..2248                     /label="cPPT"     misc_feature    2391..2708                     /label="TRE"     promoter        2709..2828                     /label="CMV2_promoter"     misc_feature    2740..2759                     /label="pCEP_fwd_primer"     misc_feature    2742..2766                     /label="LNCX_primer"     CDS             2851..3933                     /label="ORF frame 1"     misc_feature    3960..4547                     /label="WPRE"     CDS             4048..5097                     /label="ORF frame 1"     CDS             4061..4573                     /label="ORF frame 2"     misc_feature    complement(4550..4566)                     /label="pBluescriptKS_primer"     misc_feature    4734..4749                     /label="cPPT"     misc_feature    4734..4755                     /label="U3PPT"     misc_feature    5071..5251                     /label="HIV-1_5_LTR"     misc_feature    5071..5251                     /label="truncHIV-1_3_LTR"     misc_feature    complement(5277..5294)                     /label="BGH_rev_primer"     terminator      5280..5507                     /label="bGH_PA_terminator"     rep_origin      5570..5876                     /label="f1_origin"     misc_feature    complement(5990..6010)                     /label="pBABE_3_primer"     misc_feature    complement(5996..6211)                     /label="SV40_enhancer"     promoter        6008..6276                     /label="SV40_promoter"     rep_origin      6175..6252                     /label="SV40_origin"     misc_feature    6237..6256                     /label="SV40pro_F_primer"     promoter        6370..6437                     /label="EM7_promoter"     misc_feature    6438..6812                     /label="sh_ble"     gene            6438..6809                     /label="bleo"                     /gene="bleo"     terminator      6945..7064                     /label="SV40_PA_terminator"     misc_feature    7033..7052                     /label="EBV_rev_primer"      promoter        complement(7108..7126)                     /label="M13_reverse_primer"     misc_feature    complement(7125..7147)                     /label="M13_pUC_rev_primer"     promoter        complement(7161..7190)                     /label="lac_promoter"     gene            complement(8273..9133)                     /label="Ampicillin"                     /gene="Ampicillin"     CDS             complement(8273..9133)                     /label="ORF frame 3"     promoter        complement(9175..9203)                     /label="AmpR_promoter"ORIGIN    1 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc   61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt  121 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta  181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg  241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga  301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt  361 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg  421 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc  481 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg  541 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat  601 aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc  661 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc  721 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt  781 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa  841 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga  901 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga  961 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg 1021 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga 1081 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat 1141 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa 1201 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc 1261 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc 1321 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat 1381 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg 1441 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag 1501 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt 1561 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt 1621 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat 1681 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca 1741 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca 1801 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa 1861 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat 1921 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca 1981 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga 2041 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg 2101 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca 2161 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc 2221 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta 2281 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa 2341 aattttcggg tttattacag ggacagcaga gatccagttt ggttaatgat ctcgagttta 2401 ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat 2461 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg 2521 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc 2581 agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga 2641 aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag ctcggtaccc 2701 gggtcgagta ggcgtgtacg gtgggaggcc tatataagca gagctcgttt agtgaaccgt 2761 cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga 2821 tccagcctcc gcggccccga attcgccacc atggcgggac acctggcttc agattttgcc 2881 ttctcgcccc ctccaggtgg tggaggtgat gggccagggg ggccggagcc gggctgggtt 2941 gatcctcgga cctggctaag cttccaaggc cctcctggag ggccaggaat cgggccgggg 3001 gttgggccag gctctgaggt gtgggggatt cccccatgcc ccccgccgta tgagttctgt 3061 ggggggatgg cgtactgtgg gccccaggtt ggagtggggc tagtgcccca aggcggcttg 3121 gagacctctc agcctgaggg cgaagcagga gtcggggtgg agagcaactc cgatggggcc 3181 tccccggagc cctgcaccgt cacccctggt gccgtgaagc tggagaagga gaagctggag 3241 caaaacccgg aggagtccca ggacatcaaa gctctgcaga aagaactcga gcaatttgcc 3301 aagctcctga agcagaagag gatcaccctg ggatatacac aggccgatgt ggggctcacc 3361 ctgggggttc tatttgggaa ggtattcagc caaacgacca tctgccgctt tgaggctctg 3421 cagcttagct tcaagaacat gtgtaagctg cggcccttgc tgcagaagtg ggtggaggaa 3481 gctgacaaca atgaaaatct tcaggagata tgcaaagcag aaaccctcgt gcaggcccga 3541 aagagaaagc gaaccagtat cgagaaccga gtgagaggca acctggagaa tttgttcctg 3601 cagtgcccga aacccacact gcagcagatc agccacatcg cccagcagct tgggctcgag 3661 aaggatgtgg tccgagtgtg gttctgtaac cggcgccaga agggcaagcg atcaagcagc 3721 gactatgcac aacgagagga ttttgaggct gctgggtctc ctttctcagg gggaccagtg 3781 tcctttcctc tggccccagg gccccatttt ggtaccccag gctatgggag ccctcacttc 3841 actgcactgt actcctcggt ccctttccct gagggggaag cctttccccc tgtctctgtc 3901 accactctgg gctctcccat gcattcaaac tgagaattcg atatcaagct tatcgataat 3961 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct 4021 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg 4081 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg 4141 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt 4201 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt 4261 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg 4321 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc 4381 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat 4441 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc 4501 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga 4561 cctcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct 4621 gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag 4681 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa 4741 aaggggggac tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg 4801 atctaccaca cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc 4861 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagca agagaaggta 4921 gaagaagcca atgaaggaga gaacacccgc ttgttacacc ctgtgagcct gcatgggatg 4981 gatgacccgg agagagaagt attagagtgg aggtttgaca gccgcctagc atttcatcac 5041 atggcccgag agctgcatcc ggactgtact gggtctctct ggttagacca gatctgagcc 5101 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga 5161 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga 5221 cccttttagt cagtgtggaa aatctctagc agggcccgtt taaacccgct gatcagcctc 5281 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac 5341 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg 5401 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga 5461 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga 5521 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc 5581 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc 5641 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct 5701 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa 5761 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc 5821 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact 5881 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg 5941 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt 6001 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat 6061 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg 6121 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg 6181 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt 6241 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt 6301 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct 6361 gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca 6421 aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg 6481 acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg 6541 aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg 6601 accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt 6661 acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga 6721 ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact 6781 gcgtgcactt cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg 6841 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc 6901 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt 6961 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac 7021 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt 7081 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt 7141 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg 7201 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg 7261 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc 7321 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc 7381 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata 7441 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg 7501 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 7561 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 7621 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 7681 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt 7741 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 7801 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 7861 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 7921 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc 7981 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg 8041 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 8101 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 8161 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa 8221 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat 8281 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct 8341 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg 8401 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag 8461 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta 8521 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg 8581 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg 8641 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct 8701 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta 8761 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg 8821 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc 8881 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg 8941 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga 9001 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg 9061 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat 9121 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc 9181 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca 9241 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg 9301 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc 9361 ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg 9421 cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcg