FUW-tetO-hMYC载体

LOCUS       FUW-tetO-hMYC	9786 bp 	DNA	SYNDEFINITION  FUW-tetO-hMYCACCESSION   KEYWORDS    SOURCE        ORGANISM  other sequences; artificial sequences; vectors.FEATURES             Location/Qualifiers     source          1..9786                     /organism="FUW-tetO-hMYC"                     /mol_type="other DNA"     promoter        8..584                     /label="CMV_immearly_promoter"     misc_feature    87..374                     /label="CAG_enhancer"     misc_feature    541..561                     /label="CMV_fwd_primer"     misc_feature    604..784                     /label="truncHIV-1_3_LTR"     misc_feature    604..784                     /label="HIV-1_5_LTR"     misc_feature    895..939                     /label="HIV-1_psi_pack"     CDS             1327..2214                     /label="ORF frame 1"     misc_feature    1449..1682                     /label="RRE"     misc_feature    2213..2228                     /label="cPPT"       misc_feature    2371..2688                     /label="TRE"     promoter        2689..2808                     /label="CMV2_promoter"      misc_feature    2720..2739                     /label="pCEP_fwd_primer"     misc_feature    2722..2746                     /label="LNCX_primer"     CDS             2903..4222                     /label="ORF frame 2"     misc_feature    4130..4159                     /label="Myc"     misc_feature    4249..4836                     /label="WPRE"     CDS             4337..5386                     /label="ORF frame 2"     CDS             4350..4862                     /label="ORF frame 3"     misc_feature    complement(4839..4855)                     /label="pBluescriptKS_primer"     misc_feature    5023..5044                     /label="U3PPT"     misc_feature    5023..5038                     /label="cPPT"     misc_feature    5360..5540                     /label="truncHIV-1_3_LTR"     misc_feature    5360..5540                     /label="HIV-1_5_LTR"     misc_feature    complement(5566..5583)                     /label="BGH_rev_primer"     terminator      5569..5796                     /label="bGH_PA_terminator"     rep_origin      5859..6165                     /label="f1_origin"     misc_feature    complement(6279..6299)                     /label="pBABE_3_primer"     misc_feature    complement(6285..6500)                     /label="SV40_enhancer"     promoter        6297..6565                     /label="SV40_promoter"     rep_origin      6464..6541                     /label="SV40_origin"     misc_feature    6526..6545                     /label="SV40pro_F_primer"     promoter        6659..6726                     /label="EM7_promoter"     gene            6727..7098                     /label="bleo"                     /gene="bleo"     misc_feature    6727..7101                     /label="sh_ble"     terminator      7234..7353                     /label="SV40_PA_terminator"     misc_feature    7322..7341                     /label="EBV_rev_primer"     promoter        complement(7397..7415)                     /label="M13_reverse_primer"     misc_feature    complement(7414..7436)                     /label="M13_pUC_rev_primer"     promoter        complement(7450..7479)                     /label="lac_promoter"     gene            complement(8562..9422)                     /label="Ampicillin"                     /gene="Ampicillin"     CDS             complement(8562..9422)                     /label="ORF frame 2"     promoter        complement(9464..9492)                     /label="AmpR_promoter"ORIGIN    1 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc   61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac  121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa  181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag  241 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc  301 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct  361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg  421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt  481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga  541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt  601 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac  661 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg  721 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct  781 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac  841 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta  901 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta  961 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga 1021 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta 1081 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat 1141 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt 1201 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag 1261 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga 1321 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa 1381 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca 1441 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca 1501 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag 1561 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc 1621 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc 1681 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct 1741 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac 1801 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag 1861 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg 1921 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg 1981 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat 2041 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga 2101 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg 2161 gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa 2221 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat 2281 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag 2341 ggacagcaga gatccagttt ggttaatgat ctcgagttta ccactcccta tcagtgatag 2401 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag 2461 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag 2521 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa 2581 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc 2641 tatcagtgat agagaaaagt gaaagtcgag ctcggtaccc gggtcgagta ggcgtgtacg 2701 gtgggaggcc tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagacgcca 2761 tccacgctgt tttgacctcc atagaagaca ccgggaccga tccagcctcc gcggccccga 2821 attcctctga aaggctctcc ttgcagctgc ttagacgctg gatttttttc gggtagtgga 2881 aaaccagcag cctcccgcga cgatgcccct caacgttagc ttcaccaaca ggaactatga 2941 cctcgactac gactcggtgc agccgtattt ctactgcgac gaggaggaga acttctacca 3001 gcagcagcag cagagcgagc tgcagccccc ggcgcccagc gaggatatct ggaagaaatt 3061 cgagctgctg cccaccccgc ccctgtcccc tagccgccgc tccgggctct gctcgccctc 3121 ctacgttgcg gtcacaccct tctcccttcg gggagacaac gacggcggtg gcgggagctt 3181 ctccacggcc gaccagctgg agatggtgac cgagctgctg ggaggagaca tggtgaacca 3241 gagtttcatc tgcgacccgg acgacgagac cttcatcaaa aacatcatca tccaggactg 3301 tatgtggagc ggcttctcgg ccgccgccaa gctcgtctca gagaagctgg cctcctacca 3361 ggctgcgcgc aaagacagcg gcagcccgaa ccccgcccgc ggccacagcg tctgctccac 3421 ctccagcttg tacctgcagg atctgagcgc cgccgcctca gagtgcatcg acccctcggt 3481 ggtcttcccc taccctctca acgacagcag ctcgcccaag tcctgcgcct cgcaagactc 3541 cagcgccttc tctccgtcct cggattctct gctctcctcg acggagtcct ccccgcaggg 3601 cagccccgag cccctggtgc tccatgagga gacaccgccc accaccagca gcgactctga 3661 ggaggaacaa gaagatgagg aagaaatcga tgttgtttct gtggaaaaga ggcaggctcc 3721 tggcaaaagg tcagagtctg gatcaccttc tgctggaggc cacagcaaac ctcctcacag 3781 cccactggtc ctcaagaggt gccacgtctc cacacatcag cacaactacg cagcgcctcc 3841 ctccactcgg aaggactatc ctgctgccaa gagggtcaag ttggacagtg tcagagtcct 3901 gagacagatc agcaacaacc gaaaatgcac cagccccagg tcctcggaca ccgaggagaa 3961 tgtcaagagg cgaacacaca acgtcttgga gcgccagagg aggaacgagc taaaacggag 4021 cttttttgcc ctgcgtgacc agatcccgga gttggaaaac aatgaaaagg cccccaaggt 4081 agttatcctt aaaaaagcca cagcatacat cctgtccgtc caagcagagg agcaaaagct 4141 catttctgaa gaggacttgt tgcggaaacg acgagaacag ttgaaacaca aacttgaaca 4201 gctacggaac tcttgtgcgt aagaattcga tatcaagctt atcgataatc aacctctgga 4261 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg 4321 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt 4381 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag 4441 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc 4501 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga 4561 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa 4621 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac 4681 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct 4741 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca 4801 gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac ctcgagacct 4861 agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg 4921 gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag 4981 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact 5041 ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac 5101 acaaggctac ttccctgatt ggcagaacta cacaccaggg ccagggatca gatatccact 5161 gacctttgga tggtgctaca agctagtacc agttgagcaa gagaaggtag aagaagccaa 5221 tgaaggagag aacacccgct tgttacaccc tgtgagcctg catgggatgg atgacccgga 5281 gagagaagta ttagagtgga ggtttgacag ccgcctagca tttcatcaca tggcccgaga 5341 gctgcatccg gactgtactg ggtctctctg gttagaccag atctgagcct gggagctctc 5401 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt 5461 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc 5521 agtgtggaaa atctctagca gggcccgttt aaacccgctg atcagcctcg actgtgcctt 5581 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg 5641 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt 5701 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca 5761 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct 5821 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg 5881 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt 5941 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc 6001 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg 6061 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg 6121 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct 6181 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg 6241 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg 6301 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc 6361 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca 6421 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc 6481 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc 6541 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct 6601 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt 6661 gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac 6721 taaaccatgg ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga 6781 gcggtcgagt tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc 6841 gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg 6901 ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg 6961 tcggaggtcg tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc 7021 gagcagccgt gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc 7081 gtggccgagg agcaggactg acacgtgcta cgagatttcg attccaccgc cgccttctat 7141 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg 7201 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac 7261 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 7321 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc 7381 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca 7441 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg 7501 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg 7561 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc 7621 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta 7681 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag 7741 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg 7801 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg 7861 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg 7921 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga 7981 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc 8041 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt 8101 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact 8161 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg 8221 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt 8281 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt 8341 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct 8401 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg 8461 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt 8521 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt 8581 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc 8641 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg 8701 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc 8761 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg 8821 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca 8881 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga 8941 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct 9001 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg 9061 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca 9121 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata 9181 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct 9241 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact 9301 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa 9361 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc 9421 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga 9481 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga 9541 aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca 9601 gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg 9661 aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca 9721 attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca 9781 gatata//