pDR195载体

LOCUS       pDR195	6318 bp 	DNA	SYNDEFINITION  pDR195ACCESSION   KEYWORDS    SOURCE        ORGANISM  other sequences; artificial sequences; vectors.FEATURES             Location/Qualifiers     source          1..6318                     /organism="pDR195"                     /mol_type="other DNA"     terminator      39..350                     /label="yADH2_terminator"     misc_feature    complement(43..66)                     /label="pJG4_5_rev_primer"     terminator      164..355                     /label="yADH1_terminator"     misc_feature    complement(219..243)                     /label="pHybLex_rev_primer"     misc_feature    360..515                     /label="lacZ_a"     promoter        complement(363..379)                     /label="M13_forward20_primer"     misc_feature    complement(372..394)                     /label="M13_pUC_fwd_primer"     rep_origin      complement(815..1979)                     /label="2micron2_origin"     CDS             complement(2272..3075)                     /label="ORF frame 1"                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL                     LELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVK                     LQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLS                     TGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYR                     TVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN*"     gene            complement(2275..3075)                     /label="URA3"                     /gene="URA3"                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL                     LELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVK                     LQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLS                     TGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYR                     TVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN*"     CDS             2564..3040                     /label="ORF frame 2"                     /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP                     GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM                     EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"     promoter        3437..3465                     /label="AmpR_promoter"                     /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP                     GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM                     EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"     gene            3507..4367                     /label="Ampicillin"                     /gene="Ampicillin"                     /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP                     GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM                     EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"     CDS             3507..4367                     /label="ORF frame 3"                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA                     EIGASLIKHW*"     rep_origin      4522..5141                     /label="pBR322_origin"                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA                     EIGASLIKHW*"     promoter        5450..5479                     /label="lac_promoter"                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA                     EIGASLIKHW*"     misc_feature    5493..5515                     /label="M13_pUC_rev_primer"                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA                     EIGASLIKHW*"     promoter        5514..5532                     /label="M13_reverse_primer"                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA                     EIGASLIKHW*"ORIGIN    1 cccagcctcg agcggccgcg cggatccagc tttggacttc ttcgccagag gtttggtcaa   61 gtctccaatc aaggttgtcg gcttgtctac cttgccagaa atttacgaaa agatggaaaa  121 gggtcaaatc gttggtagat acgttgttga cacttctaaa taagcgaatt tcttatgatt  181 tatgattttt attattaaat aagttataaa aaaaataagt gtatacaaat tttaaagtga  241 ctcttaggtt ttaaaacgar aattcttatt cttgagtaac tctttcctgt aggtcaggtt  301 gctttctcag gtatagcatg aggtcgctct tattgaccac acctctaccg gcatgccaat  361 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat  421 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat  481 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc  541 cttacgcatc tgtgcggtat ttcacaccgc ataatcggat cgtacttgtt acccatcatt  601 gaattttgaa catccgaacc tgggagtttt ccctgaaaca gatagtatat ttgaacctgt  661 ataataatat atagtctagc gctttacgga agacaatgta tgtatttcgg ttcctggaga  721 aactattgca tctattgcat aggtaatctt gcacgtcgca tccccggttc attttctgcg  781 tttccatctt gcacttcaat agcatatctt tgttaacgaa gcatctgtgc ttcattttgt  841 agaacaaaaa tgcaacgcga gagcgctaat ttttcaaaca aagaatctga gctgcatttt  901 tacagaacag aaatgcaacg cgaaagcgct attttaccaa cgaagaatct gtgcttcatt  961 tttgtaaaac aaaaatgcaa cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc 1021 atttttacag aacagaaatg caacgcgaga gcgctatttt accaacaaag aatctatact 1081 tcttttttgt tctacaaaaa tgcatcccga gagcgctatt tttctaacaa agcatcttag 1141 attacttttt ttctcctttg tgcgctctat aatgcagtct cttgataact ttttgcactg 1201 taggtccgtt aaggttagaa gaaggctact ttggtgtcta ttttctcttc cataaaaaaa 1261 gcctgactcc acttcccgcg tttactgatt actagcgaag ctgcgggtgc attttttcaa 1321 gataaaggca tccccgatta tattctatac cgatgtggat tgcgcatact ttgtgaacag 1381 aaagtgatag cgttgatgat tcttcattgg tcagaaaatt atgaacggtt tcttctattt 1441 tgtctctata tactacgtat aggaaatgtt tacattttcg tattgttttc gattcactct 1501 atgaatagtt cttactacaa tttttttgtc taaagagtaa tactagagat aaacataaaa 1561 aatgtagagg tcgagtttag atgcaagttc aaggagcgaa aggtggatgg gtaggttata 1621 tagggatata gcacagagat atatagcaaa gagatacttt tgagcaatgt ttgtggaagc 1681 ggtattcgca atattttagt agctcgttac agtccggtgc gtttttggtt ttttgaaagt 1741 gcgtcttcag agcgcttttg gttttcaaaa gcgctctgaa gttcctatac tttctagcta 1801 gagaatagga acttcggaat aggaacttca aagcgtttcc gaaaacgagc gcttccgaaa 1861 atgcaacgcg agctgcgcac atacagctca ctgttcacgt cgcacctata tctgcgtgtt 1921 gcctgtatat atatatacat gagaagaacg gcatagtgcg tgtttatgct taaatgcgta 1981 cttatatgcg tctatttatg taggatgaaa ggtagtctag tacctcctgt gatattatcc 2041 cattccatgc ggggtatcgt atgcttcctt cagcactacc ctttagctgt tctatatgct 2101 gccactcctc aattggatta gtctcatcct tcaatgctat catttccttt gatattggat 2161 cgatccgatg ataagctgtc aaacatgaga attgggtaat aactgatata attaaattga 2221 agctctaatt tgtgagttta gtatacatgc atttacttat aatacagttt tttagttttg 2281 ctggccgcat cttctcaaat atgcttccca gcctgctttt ctgtaacgtt caccctctac 2341 cttagcatcc cttccctttg caaatagtcc tcttccaaca ataataatgt cagatcctgt 2401 agagaccaca tcatccacgg ttctatactg ttgacccaat gcgtctccct tgtcatctaa 2461 acccacaccg ggtgtcataa tcaaccaatc gtaaccttca tctcttccac ccatgtctct 2521 ttgagcaata aagccgataa caaaatcttt gtcgctcttc gcaatgtcaa cagtaccctt 2581 agtatattct ccagtagata gggagccctt gcatgacaat tctgctaaca tcaaaaggcc 2641 tctaggttcc tttgttactt cttctgccgc ctgcttcaaa ccgctaacaa tacctgggcc 2701 caccacaccg tgtgcattcg taatgtctgc ccattctgct attctgtata cacccgcaga 2761 gtactgcaat ttgactgtat taccaatgtc agcaaatttt ctgtcttcga agagtaaaaa 2821 attgtacttg gcggataatg cctttagcgg cttaactgtg ccctccatgg aaaaatcagt 2881 caagatatcc acatgtgttt ttagtaaaca aattttggga cctaatgctt caactaactc 2941 cagtaattcc ttggtggtac gaacatccaa tgaagcacac aagtttgttt gcttttcgtg 3001 catgatatta aatagcttgg cagcaacagg actaggatga gtagcagcac gttccttata 3061 tgtagctttc gacatgattt atcttcgttt cctgcatgtt tttgttctgt gcagttgggt 3121 taagaatact gggcaatttc atgtttcttc aacactacat atgcgtatat ataccaatct 3181 aagtctgtgc tccttccttc gttcttcctt ctgttcggag attaccgaat caaaaaaatt 3241 tcaaggaaac cgaaatcaaa aaaaagaata aaaaaaaaat gatgaattga aaagctaatt 3301 cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa 3361 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt 3421 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc 3481 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc 3541 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa 3601 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg 3661 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag 3721 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc 3781 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta 3841 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg 3901 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca 3961 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac 4021 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat 4081 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg 4141 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata 4201 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta 4261 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa 4321 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag 4381 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg 4441 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact 4501 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg 4561 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc 4621 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata 4681 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta 4741 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc 4801 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg 4861 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac 4921 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg 4981 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt 5041 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct 5101 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg 5161 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata 5221 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca 5281 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc 5341 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg 5401 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta 5461 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca 5521 gctatgacca tgattacgcc aagcttcctg aaacggagaa acataaacag gcattgctgg 5581 gatcacccat acatcactct gttttgcctg accttttccg gtaatttgaa aacaaacccg 5641 gtctcgaagc ggagatccgg cgataattac cgcagaaata aacccataca cgagacgtag 5701 aaccagccgc acatggccgg agaaactcct gcgagaattt cgtaaactcg cgcgcattgc 5761 atctgtattt cctaatgcgg cacttccagg cctcgatcga gaccgtttat ccattgcttt 5821 tttgttgtct ttttccctcg ttcacagaaa gtctgaagaa gctatagtag aactatgagc 5881 tttttttgtt tctgttttcc tttttttttt ttttacctct gtggaaattg ttactctcac 5941 actctttagt tcgtttgttt gttttgttta ttccaattat gaccggtgac gaaacgtggt 6001 cgatggtggg taccgcttat gctcccctcc attagtttcg attatataaa aaggccaaat 6061 attgtattat tttcaaatgt cctatcatta tcgtctaaca tctaatttct cttaaatttt 6121 ttctctttct ttcctataac accaatagtg aaaatctttt tttcttctat atctacaaaa 6181 actttttttt tctatcaacc tcgttgataa attttttctt taacaatcgt taataattaa 6241 ttaattggaa aataaccatt ttttctctct tttatacaca cattcaaaag aaagaaaaaa 6301 aatatacccc agcctcga