| 出品公司: | ZYbscience |
|---|---|
| 载体名称: | pRL-SV40 |
| 质粒类型: | 哺乳细胞表达；启动子增强子报告系统 |
| 高拷贝/低拷贝: | -- |
| 启动子: | SV40 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 3705bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素(Ampicillin) |
| 筛选标记: | -- |
| 备注: | pRL-SV40是荧光素酶报告载体，含有SV40增强子。 |
| 产品目录号: | ZY2231 |
| 稳定性: | 瞬表达 |
| 组成型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
产品详情
文献和实验
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保存条件 ：-20℃低温保存
保质期 ：三年
英文名 ：pRL-SV40
库存 ：20
供应商 ：泽叶生物
载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pRL-SV40 3705 bp DNA SYNDEFINITION pRL-SV40ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors.FEATURES Location/Qualifiers source 1..3705 /organism="pRL-SV40" /mol_type="other DNA" misc_feature complement(85..105) /label="pBABE_3_primer" misc_feature complement(91..306) /label="SV40_enhancer" promoter 103..371 /label="SV40_promoter" rep_origin 270..347 /label="SV40_origin" misc_feature 332..351 /label="SV40pro_F_primer" misc_feature 489..621 /label="chim_int" promoter 666..684 /label="T7_promoter" CDS 694..1629 /label="ORF frame 1" terminator complement(1657..1776) /label="SV40_PA_terminator" misc_feature complement(1671..1690) /label="EBV_rev_primer" promoter 1949..1977 /label="AmpR_promoter" gene 2019..2879 /label="Ampicillin" /gene="Ampicillin" CDS 2019..2879 /label="ORF frame 3" rep_origin 3034..3653 /label="pBR322_origin"ORIGIN 1 AGATCTGCGC AGCACCATGG CCTGAAATAA CCTCTGAAAG AGGAACTTGG TTAGGTACCT 61 TCTGAGGCGG AAAGAACCAG CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG 121 GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG 181 GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG 241 CAACCATAGT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC 301 ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG 361 CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA 421 AGCTTGATTC TTCTGACACA ACAGTCTCGA ACTTAAGCTG CAGAAGTTGG TCGTGAGGCA 481 CTGGGCAGGT AAGTATCAAG GTTACAAGAC AGGTTTAAGG AGACCAATAG AAACTGGGCT 541 TGTCGAGACA GAGAAGACTC TTGCGTTTCT GATAGGCACC TATTGGTCTT ACTGACATCC 601 ACTTTGCCTT TCTCTCCACA GGTGTCCACT CCCAGTTCAA TTACAGCTCT TAAGGCTAGA 661 GTACTTAATA CGACTCACTA TAGGCTAGCC ACCATGACTT CGAAAGTTTA TGATCCAGAA 721 CAAAGGAAAC GGATGATAAC TGGTCCGCAG TGGTGGGCCA GATGTAAACA AATGAATGTT 781 CTTGATTCAT TTATTAATTA TTATGATTCA GAAAAACATG CAGAAAATGC TGTTATTTTT 841 TTACATGGTA ACGCGGCCTC TTCTTATTTA TGGCGACATG TTGTGCCACA TATTGAGCCA 901 GTAGCGCGGT GTATTATACC AGACCTTATT GGTATGGGCA AATCAGGCAA ATCTGGTAAT 961 GGTTCTTATA GGTTACTTGA TCATTACAAA TATCTTACTG CATGGTTTGA ACTTCTTAAT 1021 TTACCAAAGA AGATCATTTT TGTCGGCCAT GATTGGGGTG CTTGTTTGGC ATTTCATTAT 1081 AGCTATGAGC ATCAAGATAA GATCAAAGCA ATAGTTCACG CTGAAAGTGT AGTAGATGTG 1141 ATTGAATCAT GGGATGAATG GCCTGATATT GAAGAAGATA TTGCGTTGAT CAAATCTGAA 1201 GAAGGAGAAA AAATGGTTTT GGAGAATAAC TTCTTCGTGG AAACCATGTT GCCATCAAAA 1261 ATCATGAGAA AGTTAGAACC AGAAGAATTT GCAGCATATC TTGAACCATT CAAAGAGAAA 1321 GGTGAAGTTC GTCGTCCAAC ATTATCATGG CCTCGTGAAA TCCCGTTAGT AAAAGGTGGT 1381 AAACCTGACG TTGTACAAAT TGTTAGGAAT TATAATGCTT ATCTACGTGC AAGTGATGAT 1441 TTACCAAAAA TGTTTATTGA ATCGGACCCA GGATTCTTTT CCAATGCTAT TGTTGAAGGT 1501 GCCAAGAAGT TTCCTAATAC TGAATTTGTC AAAGTAAAAG GTCTTCATTT TTCGCAAGAA 1561 GATGCACCTG ATGAAATGGG AAAATATATC AAATCGTTCG TTGAGCGAGT TCTCAAAAAT 1621 GAACAATAAT TCTAGAGCGG CCGCTTCGAG CAGACATGAT AAGATACATT GATGAGTTTG 1681 GACAAACCAC AACTAGAATG CAGTGAAAAA AATGCTTTAT TTGTGAAATT TGTGATGCTA 1741 TTGCTTTATT TGTAACCATT ATAAGCTGCA ATAAACAAGT TAACAACAAC AATTGCATTC 1801 ATTTTATGTT TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG TAAAACCTCT 1861 ACAAATGTGG TAAAATCGAT AAGGATCCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA 1921 CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC 1981 CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG 2041 TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC 2101 TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG 2161 ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA 2221 GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC 2281 AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG 2341 AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA 2401 GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG 2461 CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA 2521 ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT 2581 TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT 2641 GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT 2701 TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG 2761 GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA 2821 TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC 2881 TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA 2941 AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT 3001 TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT 3061 TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT 3121 GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC 3181 AGATACCAAA TACTGTTCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG 3241 TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG 3301 ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT 3361 CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC 3421 TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG 3481 ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG 3541 GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT 3601 TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT 3661 TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGGC TCGAC
上海泽叶生物科技有限公司
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入驻年限：9年
