| 出品公司: | EMD Biosciences (Novagen) |
|---|---|
| 别名: | pET30b, pet 30b |
| 质粒类型: | 大肠杆菌蛋白表达 |
| 表达水平: | 高 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 5421 bp |
| 5' 测序引物: | T7 |
| 5' 测序引物序列: | 5'-TAATACGACTCACTATAGGG-3' |
| 载体标签: | N-His, N-S, N-Thrombin, N-EK, C-His |
| 载体抗性: | Kanamycin |
| 表达宿主菌: | BL21(DE3) 、BLR(DE3)、BL21(DE3)pLysS |
| 备注: | N term thrombin cleavage site; N term enterokinase cleavage site; a,b,c vary by MCS |
| 产品目录号: | ZY69910-3 |
| 稳定性: | 瞬时表达 Transient |
| 组成型: | 组成型 Constitutive |
| 病毒/非病毒: | 非病毒 |
产品详情
文献和实验
相关推荐
保存条件 ：-20℃低温保存
保质期 ：三年
英文名 ：pET-30b(+)
库存 ：20
供应商 ：泽叶生物
pET30b载体基本信息
pET30b载体质粒图谱和多克隆位点信息
pET30b载体简介
The pET-30a-c(+) vectors carry an N-terminal His• Tag®/ thrombin/ S• Tag™/ enterokinase configuration plus an optional C-terminal His• Tag sequence. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand. Therefore, single-stranded sequencing should be performed using the T7 terminator primer (Cat. No. 69337-3).
pET30b载体序列
LOCUS pET-30 b (+) 5421 bp DNA circular SYNDEFINITION pET-30 b (+)ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors.COMMENT This file is created by Vector NTI http://www.biofeng.com/COMMENT ORIGDB|GenBankCOMMENT VNTAUTHORNAME|biofeng.com|COMMENT VNTOAUTHORAD1|Novagen Inc.|FEATURES Location/Qualifiers source 1..5421 /organism="pET-30 b (+)" /mol_type="other DNA" terminator complement(1..129) /label="T7_terminator" misc_feature 69..87 /label="T7_Terminal_primer" misc_feature complement(218..229) /label="EK" misc_feature 301..1907 /label="lacI" CDS 326..1075 /label="ORF frame 2" misc_feature complement(355..371) /label="T7_transl_en_RBS" misc_feature complement(389..416) /label="lacO" promoter complement(416..434) /label="T7_promoter" gene 470..733 /label="tet (300 - 563)" /gene="tet (300 - 563)" misc_feature complement(522..541) /label="pBRrevBam_primer" CDS 948..1907 /label="ORF frame 3" gene 1966..2241 /label="tet (576 - 851)" /gene="tet (576 - 851)" misc_feature 2716..2907 /label="ROP" misc_feature complement(2901..2923) /label="pGEX_3_primer" rep_origin complement(3322..3941) /label="pBR322_origin" gene 4047..4862 /label="KanR2" /gene="KanR2" CDS 4047..4862 /label="ORF frame 3"ORIGIN 1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 181 cgacggagct cgaattcgga tccgatatcg ccatggcctt gtcgtcgtcg tcggtaccca 241 gatctgggct gtccatgtgc tggcgttcga atttagcagc agcggtttct ttcataccag 301 aaccgcgtgg caccagacca gaagaatgat gatgatgatg gtgcatatgt atatctcctt 361 cttaaagtta aacaaaatta tttctagagg ggaattgtta tccgctcaca attcccctat 421 agtgagtcgt attaatttcg cgggatcgag atcgatctcg atcctctacg ccggacgcat 481 cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac 541 cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat 601 ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct 661 tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc 721 gcataaggga gagcgtcgag atcccggaca ccatcgaatg gcgcaaaacc tttcgcggta 781 tggcatgata gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgt 841 tatacgatgt cgcagagtat gccggtgtct cttatcagac cgtttcccgc gtggtgaacc 901 aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg gcggagctga 961 attacattcc caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg ctgattggcg 1021 ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc 1081 gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag 1141 cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg atcattaact 1201 atccgctgga tgaccaggat gccattgctg tggaagctgc ctgcactaat gttccggcgt 1261 tatttcttga tgtctctgac cagacaccca tcaacagtat tattttctcc catgaagacg 1321 gtacgcgact gggcgtggag catctggtcg cattgggtca ccagcaaatc gcgctgttag 1381 cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat aaatatctca 1441 ctcgcaatca aattcagccg atagcggaac gggaaggcga ctggagtgcc atgtccggtt 1501 ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca 1561 acgatcagat ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg 1621 cggacatctc ggtagtggga tacgacgata ccgaagacag ctcatgttat atcccgccgt 1681 taaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc 1741 aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa 1801 gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat 1861 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt 1921 aatgtaagtt agctcactca ttaggcaccg ggatctcgac cgatgccctt gagagccttc 1981 aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact 2041 gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctctgggt cattttcggc 2101 gaggaccgct ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt attcggaatc 2161 ttgcacgccc tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt cggcgagaag 2221 caggccatta tcgccggcat ggcggcccca cgggtgcgca tgatcgtgct cctgtcgttg 2281 aggacccggc taggctggcg gggttgcctt actggttagc agaatgaatc accgatacgc 2341 gagcgaacgt gaagcgactg ctgctgcaaa acgtctgcga cctgagcaac aacatgaatg 2401 gtcttcggtt tccgtgtttc gtaaagtctg gaaacgcgga agtcagcgcc ctgcaccatt 2461 atgttccgga tctgcatcgc aggatgctgc tggctaccct gtggaacacc tacatctgta 2521 ttaacgaagc gctggcattg accctgagtg atttttctct ggtcccgccg catccatacc 2581 gccagttgtt taccctcaca acgttccagt aaccgggcat gttcatcatc agtaacccgt 2641 atcgtgagca tcctctctcg tttcatcggt atcattaccc ccatgaacag aaatccccct 2701 tacacggagg catcagtgac caaacaggaa aaaaccgccc ttaacatggc ccgctttatc 2761 agaagccaga cattaacgct tctggagaaa ctcaacgagc tggacgcgga tgaacaggca 2821 gacatctgtg aatcgcttca cgaccacgct gatgagcttt accgcagctg cctcgcgcgt 2881 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt 2941 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg 3001 tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact 3061 atgcggcatc agagcagatt gtactgagag tgcaccatat atgcggtgtg aaataccgca 3121 cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc 3181 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 3241 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 3301 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 3361 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 3421 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 3481 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 3541 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 3601 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 3661 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 3721 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac 3781 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 3841 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 3901 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 3961 tcagtggaac gaaaactcac gttaagggat tttggtcatg aacaataaaa ctgtctgctt 4021 acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg tcttgctcta 4081 ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata 4141 atgtcgggca atcaggtgcg acaatctatc gattgtatgg gaagcccgat gcgccagagt 4201 tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac 4261 taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg 4321 atgatgcatg gttactcacc actgcgatcc ccgggaaaac agcattccag gtattagaag 4381 aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc 4441 attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg 4501 cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg 4561 gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc tcaccggatt 4621 cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa 4681 taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc 4741 tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg 4801 gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct 4861 aagaattaat tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 4921 gttccgcgca catttccccg aaaagtgcca cctgaaattg taaacgttaa tattttgtta 4981 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc 5041 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg 5101 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat 5161 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc 5221 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag 5281 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg 5341 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta 5401 cagggcgcgt cccattcgcc a
上海泽叶生物科技有限公司
实名认证
钻石会员
入驻年限：9年
