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The Human TP53 Gene qBiomarker Somatic Mutation PCR Array allows the detection of 92 unique mutations in the human TP53 gene. TP53, a tumor suppressor gene and transcription factor, responds to diverse cellular stresses to regulate target genes that induce apoptosis, cell cycle arrest, DNA repair, genomic stability, and senescence. Previous scientific reports have shown that more than half of all cancers contain at least one mutation in the TP53 gene. The TP53 mutations found in cancer samples are predominately missense point mutations in the DNA-binding domain that disrupt either contact with DNA or the protein’s structure. The DNA sequence mutation assays in the array detect the most frequent mutations in TP53 compiled from over 22,000 reported cancer sample sequences. Each mutation assay is bench-tested for the sensitive detection of a low percentage of mutation-containing DNA in a background of wild-type genomic DNA. The 96-well format of this array profiles the mutation status of 1 sample, while the 384-well array format profiles 4 samples, and each set of plates includes qPCR mastermix. Using real-time PCR, research studies can easily and reliably detect a panel of mutations in the TP53 gene with this array.
| Nucleotide Change | Amino Acid Change | Mutation Frequency |
| c.524G>A | p.R175H | 0.031 |
| c.743G>A | p.R248Q | 0.021 |
| c.818G>A | p.R273H | 0.020 |
| c.742C>T | p.R248W | 0.018 |
| c.817C>T | p.R273C | 0.017 |
| c.844C>T | p.R282W | 0.015 |
| c.747G>T | p.R249S | 0.012 |
| c.733G>A | p.G245S | 0.012 |
| c.659A>G | p.Y220C | 0.008 |
| c.637C>T | p.R213* | 0.008 |
| c.469G>T | p.V157F | 0.006 |
| c.586C>T | p.R196* | 0.005 |
| c.527G>T | p.C176F | 0.004 |
| c.536A>G | p.H179R | 0.004 |
| c.488A>G | p.Y163C | 0.004 |
| c.853G>A | p.E285K | 0.004 |
| c.734G>A | p.G245D | 0.004 |
| c.818G>T | p.R273L | 0.004 |
| c.701A>G | p.Y234C | 0.003 |
| c.722C>T | p.S241F | 0.003 |
| c.535C>T | p.H179Y | 0.003 |
| c.578A>G | p.H193R | 0.003 |
| c.814G>A | p.V272M | 0.003 |
| c.574C>T | p.Q192* | 0.003 |
| c.422G>A | p.C141Y | 0.003 |
| c.725G>T | p.C242F | 0.003 |
| c.743G>T | p.R248L | 0.003 |
| c.451C>T | p.P151S | 0.003 |
| c.584T>C | p.I195T | 0.003 |
| c.711G>A | p.M237I | 0.002 |
| c.455C>T | p.P152L | 0.002 |
| c.473G>A | p.R158H | 0.002 |
| c.614A>G | p.Y205C | 0.002 |
| c.839G>C | p.R280T | 0.002 |
| c.527G>A | p.C176Y | 0.002 |
| c.833C>T | p.P278L | 0.002 |
| c.473G>T | p.R158L | 0.002 |
| c.734G>T | p.G245V | 0.002 |
| c.404G>A | p.C135Y | 0.002 |
| c.856G>A | p.E286K | 0.002 |
| c.646G>A | p.V216M | 0.002 |
| c.713G>A | p.C238Y | 0.002 |
| c.733G>T | p.G245C | 0.002 |
| c.832C>T | p.P278S | 0.002 |
| c.1024C>T | p.R342* | 0.002 |
| c.824G>A | p.C275Y | 0.002 |
| c.707A>G | p.Y236C | 0.002 |
| c.481G>A | p.A161T | 0.002 |
| c.641A>G | p.H214R | 0.002 |
| c.797G>A | p.G266E | 0.002 |
| c.839G>A | p.R280K | 0.002 |
| c.517G>T | p.V173L | 0.002 |
| c.517G>A | p.V173M | 0.002 |
| c.725G>A | p.C242Y | 0.002 |
| c.772G>A | p.E258K | 0.002 |
| c.461G>T | p.G154V | 0.002 |
| c.730G>A | p.G244S | 0.002 |
| c.749C>T | p.P250L | 0.002 |
| c.880G>T | p.E294* | 0.002 |
| c.892G>T | p.E298* | 0.002 |
| c.404G>T | p.C135F | 0.001 |
| c.730G>T | p.G244C | 0.001 |
| c.731G>A | p.G244D | 0.001 |
| c.395A>G | p.K132R | 0.001 |
| c.536A>T | p.H179L | 0.001 |
| c.824G>T | p.C275F | 0.001 |
| c.437G>A | p.W146* | 0.001 |
| c.438G>A | p.W146* | 0.001 |
| c.797G>T | p.G266V | 0.001 |
| c.476C>T | p.A159V | 0.001 |
| c.836G>A | p.G279E | 0.001 |
| c.578A>T | p.H193L | 0.001 |
| c.713G>T | p.C238F | 0.001 |
| c.715A>G | p.N239D | 0.001 |
| c.430C>T | p.Q144* | 0.001 |
| c.581T>G | p.L194R | 0.001 |
| c.796G>A | p.G266R | 0.001 |
| c.577C>T | p.H193Y | 0.001 |
| c.711G>T | p.M237I | 0.001 |
| c.736A>G | p.M246V | 0.001 |
| c.406C>T | p.Q136* | 0.001 |
| c.638G>T | p.R213L | 0.001 |
| c.746G>T | p.R249M | 0.001 |
| c.833C>G | p.P278R | 0.001 |
| c.844C>G | p.R282G | 0.001 |
| c.452C>A | p.P151H | 0.001 |
| c.467G>C | p.R156P | 0.001 |
| c.722C>G | p.S241C | 0.001 |
| c.745A>G | p.R249G | 0.001 |
| c.818G>C | p.R273P | 0.001 |
| c.396G>C | p.K132N | 0.001 |
| c.745A>T | p.R249W | 0.001 |
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