EGFR Gene Mutation PCR Array

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The Human EGFR Gene qBiomarker Somatic Mutation PCR Array allows the detection of 44 unique mutations in the human EGFR gene. EGFR, a proto-oncogene and a receptor tyrosine kinase, activates MAP kinase, AKT, and JNK signaling pathways to regulate cellular migration, adhesion, and proliferation. Previous scientific reports have shown a strong association between mutations in the EGFR gene and lung cancer as well as glioblastoma multiforme. The EGFR mutations found in cancer samples include missense point mutations and inactivating nonsense mutations in the P-loop, activation loop, and kinase domains. The DNA sequence mutation assays in the array detect the most frequent mutations in EGFR compiled from over 12,250 reported cancer sample sequences. Each mutation assay is bench-tested for the sensitive detection of a low percentage of mutation-containing DNA in a background of wild-type genomic DNA. The 96-well format of this array profiles the mutation status of 2 samples, while the 384-well array format profiles 8 samples, and each set of plates includes qPCR mastermix. Using real-time PCR, research studies can easily and reliably detect a panel of mutations in the EGFR gene with this array.

Nucleotide Change	Amino Acid Change	Mutation Frequency
c.2573T>G	p.L858R	0.0976
c.2235_2249del15	p.E746_A750del	0.0423
c.2236_2250del15	p.E746_A750del	0.0236
c.2240_2257del18	p.L747_P753>S	0.0088
c.2369C>T	p.T790M	0.0061
c.2239_2248TTAAGAGAAG>C	p.L747_A750>P	0.0057
c.2237_2255>T	p.E746_S752>V	0.0035
c.2240_2254del15	p.L747_T751del	0.0033
c.2582T>A	p.L861Q	0.0027
c.2239_2256del18	p.L747_S752del	0.0021
c.2155G>A	p.G719S	0.0019
c.2303G>T	p.S768I	0.0018
c.2156G>C	p.G719A	0.0018
c.866C>T	p.A289V	0.0016
c.2239_2253del15	p.L747_T751del	0.0015
c.2239_2251>C	p.L747_T751>P	0.0015
c.2239_2247del9	p.L747_E749del	0.0014
c.2237_2251del15	p.E746_T751>A	0.0014
c.2155G>T	p.G719C	0.0013
c.2497T>G	p.L833V	0.0012
c.1793G>T	p.G598V	0.0012
c.2125G>A	p.E709K	0.0008
c.2307_2308insGCCAGCGTG	p.V769_D770insASV	0.0007
c.2126A>C	p.E709A	0.0007
c.2240_2251del12	p.L747_T751>S	0.0006
c.2239_2258>CA	p.L747_P753>Q	0.0006
c.323G>A	p.R108K	0.0005
c.89_889del801	p.V30_R297>G	0.0004
c.2203G>A	p.G735S	0.0003
c.2238_2248>GC	p.L747_A750>P	0.0003
c.2126A>G	p.E709G	0.0003
c.2238_2255del18	p.E746_S752>D	0.0003
c.787A>C	p.T263P	0.0003
c.865G>A	p.A289T	0.0002
c.2126A>T	p.E709V	0.0002
c.866C>A	p.A289D	0.0002
c.2237_2252>T	p.E746_T751>V	0.0002
c.2572C>A	p.L858M	0.0002
c.2310_2311insGGT	p.D770_N771insG	0.0002
c.2248G>C	p.A750P	0.0002
c.2429G>A	p.G810D	0.0002
c.2156G>A	p.G719D	0.0002
c.2239_2256>CAA	p.L747_S752>Q	0.0002
c.2193G>A	p.W731*	0.0002