小鼠γ干扰素包被ELISA试剂盒

价格： ¥6019

品牌：eBioscience

货号：85-BMS606

产品详情

描述

The mouse IFN-gamma ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of mouse IFN-gamma.

IFN-gamma, also called Type II interferon, is a homodimeric gycoprotein containing approximately 21 to 24 kD subunits. In contrast to IFN-gamma and IFN-gamma synthesis, which can occur in any cell, production of IFN-gamma is a function of T cells and NK cells.
Type II IFN or IFN-gamma is a lymphokine that displays no molecular homology with type I IFN, but shares some important biologic activities. Specifically, IFN induces an anti-viral state and is anti-proliferative. In addition, IFN-gamma has several properties related to immunoregulation.

(1) IFN- is a potent activator of mononuclear phagocytes, e.g. IFN-gamma stimulates the expression of Mac-1, augments endocytosis and phagocytosis by monocytes; and activates macrophages to kill tumor cells by releasing reactive oxagen intermediates and TNF-alpha.

(2) IFN-gamma induces or augments the expression of MHC antigens on macrophages, T and B cells and some tumor cell lines.

(3) On T and B cells IFN- promotes differentiation. It enhaces proliferation of activated B cells and can act synergistically with IL-2 to increase immunoglobulin light-chain synthesis. IFN-gamma is one of the natural B-cell differentiation factors.

(4) Finally, IFN-gamma activates neutrophils, NK cells and vascular endothelial cells. The role of IFN-gamma as a disease marker has been demonstrated for a number of different pathological situations.

Conjugate
Biotin

Suitable Sample Types
cell culture supernatant, serum

Sample Volume
50 µL

Reported Application
ELISA

Figures

规格

Assay Range:	15.6-1,000 pg/mL
Assay Sensitivity:	5.3 pg/mL
Detection Method:	Colorimetric
Detector Antibody Conjugate:	Biotin
For Use With (Equipment):	Microplate Reader
Former Identifiers:	Platinum, eBioscience
Gene Aliases:	IFN-g, Ifg
Gene ID:	15978
Gene Name:	interferon gamma
Gene Symbol:	IFNG
Immunoassay Kit Format:	Coated ELISA Kit
Interassay CV:	10%
Intraassay CV:	5.0%
Label or Dye:	HRP (Horseradish Peroxidase)
Product Size:	96 tests
Protein Aliases:	IFN-gamma
Protein Name:	Interferon gamma
Sample Type (Specific):	Cell Culture Supernatants, Serum
Sample Volume:	50 µL
Target (Kit Named):	IFN gamma
Target Species (Named):	Mouse
Target Species (Validated):	Mouse
UniProt ID:	P01580

内容及储存

Aluminium pouch(es) with a Microplate coated with monoclonal antibody to mouse IFN-gamma
Biotin-Conjugate anti-mouse IFN-gamma monoclonal antibody
Streptavidin-HRP
Mouse IFN-gamma Standard lyophilized, 2 ng/mL upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Adhesive Films