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注册证号 ：咨询销售
保存条件 ：-20度
保质期 ：6个月
英文名 ： GQH-DER
库存 ：大量
供应商 ：中乔新舟
CAS号 ：咨询销售
Sciencell公司产品由上海中乔新舟生物科技有限公司优质供应
美国ScienCell研究实验室（www.sciencellonline.com）成立于1999年，公司总部位于美国加州的圣地亚哥。主要致力
于实验室科研用原代细胞、原代细胞专用培养基、原代细胞无血清培养基、干细胞、干细胞培养基、干细胞无血清培养
基的研究和开发，在全球拥有众多客户。在国内销售15年来，很多老师应用其产品发表了高质量的SCI文章，且有着极高
的文献引用率。凭借着严格的质控和优秀的产品品质，深受广大科研工作者的信赖。
ScienCell研究实验室生产的原代细胞、原代细胞专用培养基都经过了严格的质量控制，细胞纯度可达98％。其中包括21
种人体正常细胞系统，90多种不同细胞类型。大多数细胞在全球唯有ScienCell实验室能够成功分离，产品质量过硬。
确保了实验结果的重复性和连贯性。
请登录Sciencell公司官方网站（www.zqxzbio.com或www.sciencellonline.com）以确保购买正规公司产品
人皮炎、哮喘定量PCR芯片试剂盒描述：
ScienCell's GeneQuery™ Human Dermatitis and Asthma qPCR Array Kit (GQH-DER) is designed to facilitate gene expression profiling of 88 key genes involved in the pathogenesis of atopic dermatitis (or eczema) and asthma, which is frequently associated with this chronic inflammatory skin disorder. Genes are also included for related conditions such as allergic contact dermatitis, seborrheic dermatitis, ichthyosis, and eczema herpeticum. Brief examples of how included genes may be grouped according to pathology are shown below:
- Atopic dermatitis: CCL11, IFNG, IL13, IL4, SOCS3, FLG
- Asthma: HNMT, ADRB2, ALOX5, PLA2G7, PHF11
- Allergic contact dermatitis: IL10, IL17A, TNF, CD4, CXCR3
- Seborrheic dermatitis: IDH2, SETBP1, ZNF750, ALOX12B, BTD
- Ichthyosis: LOR, ABC12, KRT1, KRT14, STS
- Eczema herpeticum: TSLP, IRF2, IRF7, IL7R, KLK12
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.
GeneQuery™ Human Dermatitis and Asthma qPCR Array Plate Layout* (8 controls in Bold and Italic)
| 1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
| A |
ABCA12 |
CCL11 |
CGA |
EPX |
HNMT |
IL18 |
IL9 |
LEP |
PAFAH1B1 |
SCGB3A2 |
TAC1 |
β-actin |
| B |
ADA |
CCL17 |
COL6A5 |
FAF2 |
HRH1 |
IL2 |
IRAK3 |
LOR |
PDE4A |
SELE |
TBX21 |
GAPDH |
| C |
ADRB2 |
CCL18 |
CSF2 |
FCER1A |
ICAM1 |
IL3 |
IRF2 |
LTC4S |
PHF11 |
SETBP1 |
TGM1 |
LDHA |
| D |
ALOX12B |
CCL22 |
CXCR3 |
FCER1G |
IDH2 |
IL31RA |
IRF7 |
MS4A2 |
PHYH |
SLCO2A1 |
TMEM79 |
NONO |
| E |
ALOX5 |
CCL27 |
CYSLTR1 |
FCER2 |
IFNG |
IL4 |
KLK12 |
NACA |
PLA2G7 |
SOCS3 |
TNF |
PPIH |
| F |
ALOX5AP |
CCL5 |
CYSLTR2 |
FLG |
IL10 |
IL4R |
KLK7 |
NAT2 |
PTGDR |
SPINK5 |
TSLP |
GDC |
| G |
BCL7B |
CD4 |
DPP10 |
HAVCR1 |
IL13 |
IL5 |
KRT1 |
NR3C1 |
PTGDR2 |
STAT3 |
VCAM1 |
PPC |
| H |
BTD |
CD40LG |
DSG1 |
HLA-G |
IL17A |
IL7R |
KRT14 |
ORMDL3 |
RNASE3 |
STS |
ZNF750 |
NTC |
* gene selection may be updated based on new research and development
| Catalog No. | GK048 |
|---|---|
| Country of Manufacture | United States |
| Product Code | GQH-DER |
| Size/Quantity | One 96-well plate, one 20ul reaction per well |
| Product Use | For research use only. It is not approved for human or animal use, or for application in clinical or in vitro diagnostic procedures. |
| Storage | Store at 4°C upon receipt. For long-term storage (>1 year), store the plate at -20°C in a manual defrost freezer. |
| Shipping Info | Ambient temperature. |
|
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