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注册证号 ：咨询销售
保存条件 ：-20度冻存
保质期 ：6个月
英文名 ：GQH-TMH
库存 ：大量
供应商 ：中乔新舟
CAS号 ：咨询销售
Sciencell公司产品由上海中乔新舟生物科技有限公司优质供应
美国ScienCell研究实验室（www.sciencellonline.com）成立于1999年，公司总部位于美国加州的圣地亚哥。主要致力
于实验室科研用原代细胞、原代细胞专用培养基、原代细胞无血清培养基、干细胞、干细胞培养基、干细胞无血清培养
基的研究和开发，在全球拥有众多客户。在国内销售15年来，很多老师应用其产品发表了高质量的SCI文章，且有着极高
的文献引用率。凭借着严格的质控和优秀的产品品质，深受广大科研工作者的信赖。
ScienCell研究实验室生产的原代细胞、原代细胞专用培养基都经过了严格的质量控制，细胞纯度可达98％。其中包括21
种人体正常细胞系统，90多种不同细胞类型。大多数细胞在全球唯有ScienCell实验室能够成功分离，产品质量过硬。
确保了实验结果的重复性和连贯性。
请登录Sciencell公司官方网站（www.zqxzbio.com或www.sciencellonline.com）以确保购买正规公司产品
人转录误调节在恶性血液肿瘤中的定量PCR芯片试剂盒描述：
ScienCell's GeneQuery™ Human Transcriptional Misregulation in Hematological Malignancy qPCR Array Kit (GQH-TMH) is designed to facilitate gene expression profiling of 88 key genes involved in three types hematological malignancy development (acute myeloid leukemia, precursor B lymphoblastic leukemia, and precursor T lymphoblastic leukemia), and is based on extensive literature research. Brief examples of how included genes may be grouped according to function and malignancy types are shown below:
- Cell cycle regulation: CCNT1, CCNT2, CDK9, CDK14, CDKN2C
- Signaling mediation: IGF1R, BMP2K, PROM1
- Differentiation regulation: CSF1R, CD14, DUSP6, GZMB, HOXA9, CCR7, FUT8, SIX4, EYA1
- Mutated transcription factors identified
- in acute myeloid leukemia: AML1, CEBPA, PLZF, ZBTB16, SPI1
- in precursor B lymphoblastic leukemia: TCF3, PBX1
- in precursor T lymphoblastic leukemia: TLX1, TLX3
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery™ Human Transcriptional Misregulation in Hematological Malignancy qPCR Array Plate Layout*
(8 controls in Bold and Italic)
|
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
| A |
AFF1 |
CCND2 |
CDKN1A |
DUSP6 |
HDAC1 |
HPGD |
KLF3 |
MEIS1 |
PBX1 |
RUNX2 |
TCF3 |
β-actin |
| B |
ATM |
CCNT1 |
CDKN2C |
ETV6 |
HDAC2 |
IGF1 |
KMT2A |
MLLT1 |
PER2 |
SIN3A |
TLX1 |
GAPDH |
| C |
BCL2A1 |
CCNT2 |
CEBPA |
ETV7 |
HHEX |
IGF1R |
LDB1 |
MLLT3 |
PROM1 |
SIX1 |
TLX3 |
LDHA |
| D |
BCL2L1 |
CCR7 |
CSF1R |
EYA1 |
HIST2H3C |
IL3 |
LMO2 |
MYC |
PTCRA |
SIX4 |
TRAF1 |
NONO |
| E |
BCL6 |
CD14 |
CSF2 |
FCGR1A |
HMGA2 |
ITGB7 |
LYL1 |
MYCN |
RARA |
SMAD1 |
UTY |
PPIH |
| F |
BIRC3 |
CD86 |
DDIT3 |
FUT8 |
HOXA10 |
JMJD1C |
MAF |
NCOR1 |
REL |
SP1 |
WHSC1 |
GDC |
| G |
BMP2K |
CDK14 |
DEFA3 |
GRIA3 |
HOXA11 |
JUP |
MAX |
NFKB1 |
RUNX1 |
SPI1 |
WNT16 |
PPC |
| H |
CCNA1 |
CDK9 |
DOT1L |
GZMB |
HOXA9 |
KDM6A |
MEF2C |
PAX5 |
RUNX1T1 |
SUPT3H |
ZBTB16 |
NTC |
* gene selection may be updated based on new research and development
| Catalog No. | GK030 |
|---|---|
| Country of Manufacture | United States |
| Product Code | GQH-TMH |
| Size/Quantity | One 96-well plate, one 20ul reaction per well |
| Product Use | For research use only. It is not approved for human or animal use, or for application in clinical or in vitro diagnostic procedures. |
| Storage | Store at 4°C upon receipt. For long-term storage (>1 year), store the plate at -20°C in a manual defrost freezer. |
| Shipping Info | Ambient temperature. |
|
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