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保存条件 ：-20度
保质期 ：6个月
英文名 ： GQH-MGL
库存 ：大量
供应商 ：中乔新舟
CAS号 ：咨询销售
Sciencell公司产品由上海中乔新舟生物科技有限公司优质供应
美国ScienCell研究实验室（www.sciencellonline.com）成立于1999年，公司总部位于美国加州的圣地亚哥。主要致力
于实验室科研用原代细胞、原代细胞专用培养基、原代细胞无血清培养基、干细胞、干细胞培养基、干细胞无血清培养
基的研究和开发，在全球拥有众多客户。在国内销售15年来，很多老师应用其产品发表了高质量的SCI文章，且有着极高
的文献引用率。凭借着严格的质控和优秀的产品品质，深受广大科研工作者的信赖。
ScienCell研究实验室生产的原代细胞、原代细胞专用培养基都经过了严格的质量控制，细胞纯度可达98％。其中包括21
种人体正常细胞系统，90多种不同细胞类型。大多数细胞在全球唯有ScienCell实验室能够成功分离，产品质量过硬。
确保了实验结果的重复性和连贯性。
人小神经胶质细胞生物学定量PCR芯片试剂盒描述：
ScienCell's GeneQuery™ Human Microglia Cell Biology qPCR Array (GQH-MGL) facilitates gene expression profiling of 88 key genes involved in microglial regulation and function. Microglia are considered to be the main cells of defense in the central nervous system (CNS). Though often touted as “the resident macrophages of the CNS,” microglia distinguish themselves from macrophages in regulation and through specialized roles. Examples of how included genes may be grouped according to their function are shown below:
- Classical priming: BIRC3, IFNG, IL12A, CXCL10, TYMP
- Alternative priming: GAS7, IGF1, CXCL2, SPP1, MRC1
- Microglia markers: PTPRC, ITGAX, AIF1, CD40, EDA
- Sensome: GPR34, CXCR2, ADORA3, LTF, SIGLECH
- Receptors: MARCO, MSR1, P2RX4, SIRPA, CSF1R
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.
GeneQuery™ Human Microglia Cell Biology qPCR Array Plate Layout* (8 controls in Bold and Italic)
| 1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
| A |
ADORA3 |
CD40 |
CX3CR1 |
F13A1 |
HLA-DQB1 |
IRF1 |
MARCO |
NOS2 |
PTGES |
SLCO2B1 |
TMEM8C |
β-actin |
| B |
AIF1 |
CD47 |
CXCL10 |
FGL2 |
HLA-DRA |
IRF8 |
MMP12 |
P2RX4 |
PTGS2 |
SPI1 |
TNF |
GAPDH |
| C |
BIRC3 |
CD68 |
CXCL2 |
GAS7 |
HLA-DRB1 |
ITGAM |
MRC1 |
P2RX7 |
PTPRC |
SPP1 |
TREM2 |
LDHA |
| D |
CASP1 |
CD80 |
CXCL9 |
GPR34 |
IFNG |
ITGAX |
MSR1 |
P2RY1 |
PYCARD |
TGFB1 |
TXNIP |
NONO |
| E |
CCL8 |
CD86 |
CXCR2 |
GPR84 |
IGF1 |
ITGB5 |
MYB |
P2RY12 |
RUNX1 |
TLR2 |
TYMP |
PPIH |
| F |
CD163 |
CLEC5A |
ECSCR |
HAVCR2 |
IL12A |
LGALS3 |
MYD88 |
P2RY2 |
SIGLEC5 |
TLR3 |
TYROBP |
GDC |
| G |
CD200R1 |
CLEC7A |
EDA |
HEXB |
IL1B |
LILRA5 |
NLRC4 |
PANX1 |
SIRPA |
TLR4 |
UPK1B |
PPC |
| H |
CD33 |
CSF1R |
EGR2 |
HLA-DQA1 |
IL6 |
LTF |
NLRP3 |
PILRA |
SLAMF9 |
TLR5 |
XIAP |
NTC |
* gene selection may be updated based on new research and development
| Catalog No. | GK069 |
|---|---|
| Country of Manufacture | United States |
| Product Code | GQH-MGL |
| Size/Quantity | One 96-well plate, one 20ul reaction per well |
| Product Use | GQH-MGL is for research use only. It is not approved for human or animal use, or for application in clinical or in vitro diagnostic procedures. |
| Storage | Store at 4°C upon receipt. For long-term storage (>1 year), store the plate at -20°C in a manual defrost freezer. |
| Shipping Info | The product is shipped at ambient temperature. Upon receipt, the plate should be stored at 4°C and is good for up to 12 months. For long-term storage (>1 year), store the plate at -20°C in a manual defrost freezer. |
| References | [1] Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, Speleman F. (2002) "Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes." Genome Biol. 3(7): 1-12. |
|
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