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保存条件 ：-20度保存
保质期 ：6个月
英文名 ：GQH-TRS
库存 ：大量
供应商 ：中乔新舟
CAS号 ：咨询销售
Sciencell公司产品由上海中乔新舟生物科技有限公司优质供应
美国ScienCell研究实验室（www.sciencellonline.com）成立于1999年，公司总部位于美国加州的圣地亚哥。主要致力
于实验室科研用原代细胞、原代细胞专用培养基、原代细胞无血清培养基、干细胞、干细胞培养基、干细胞无血清培养
基的研究和开发，在全球拥有众多客户。在国内销售15年来，很多老师应用其产品发表了高质量的SCI文章，且有着极高
的文献引用率。凭借着严格的质控和优秀的产品品质，深受广大科研工作者的信赖。
ScienCell研究实验室生产的原代细胞、原代细胞专用培养基都经过了严格的质量控制，细胞纯度可达98％。其中包括21
种人体正常细胞系统，90多种不同细胞类型。大多数细胞在全球唯有ScienCell实验室能够成功分离，产品质量过硬。
确保了实验结果的重复性和连贯性。
请登录Sciencell公司官方网站（www.zqxzbio.com或www.sciencellonline.com）以确保购买正规公司产品
人T细胞T细胞受体信号通路定量PCR芯片试剂盒描述：
ScienCell's GeneQuery™ Human T Cell Receptor Signaling Pathway qPCR Array Kit (GQH-TRS) is designed to facilitate gene expression profiling of 88 key genes involved in T cell receptor signaling pathway. Brief examples of how included genes may be grouped according to their functions are shown below:
- T cell receptor complex components: CD3D, CD3E, CD3G
- Co-receptors: CD4, CD8A, CD8B
- Co-inhibitors: PDCD1, CTLA4
- Co-stimulators: ICOS, CD28
- Kinases: FYN, ZAP70
- MAPK pathway: KRAS, HRAS, NRAS, RAF1, MAP2K1, MAPK1, MAPK3
- PI3K/AKT pathway: PI3Ks, AKT1, AKT2
- IKK/NFKB pathway: NFKBs, DAG1, BCL10, CARD11, MAP3K7, MALT1, CHUK, IKBKB, IKBKG
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery™ Human T Cell Receptor Signaling Pathway qPCR Array Plate Layout*
(8 controls in Bold and Italic)
|
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
| A |
AKT1 |
CD274 |
CD8A |
DYRK1A |
HRAS |
MALT1 |
NCK1 |
NFKBIE |
PDPK1 |
PPP3CB |
RELA |
β-actin |
| B |
AKT2 |
CD28 |
CD8B |
DYRK2 |
ICOS |
MAP2K1 |
NCK2 |
NRAS |
PIK3CA |
PPP3R1 |
SOS1 |
GAPDH |
| C |
BCL10 |
CD3D |
CDC42 |
FOS |
IKBKB |
MAP2K7 |
NFATC1 |
NRON |
PIK3CB |
PRKCA |
SOS2 |
LDHA |
| D |
CALM1 |
CD3E |
CDK4 |
FYN |
IKBKG |
MAP3K7 |
NFATC2 |
PAK1 |
PIK3R1 |
PRKCQ |
TEC |
NONO |
| E |
CARD11 |
CD3G |
CHUK |
GRAP2 |
ITK |
MAP3K8 |
NFATC3 |
PAK2 |
PIK3R2 |
PTCRA |
TNF |
PPIH |
| F |
CBL |
CD4 |
CTLA4 |
GRB2 |
JUN |
MAPK1 |
NFKB1 |
PAK3 |
PIK3R3 |
PTPN6 |
VAV1 |
GDC |
| G |
CBLB |
CD80 |
DAG1 |
GSK3A |
KRAS |
MAPK3 |
NFKBIA |
PAK4 |
PLCG1 |
RAF1 |
VAV2 |
PPC |
| H |
CBLC |
CD86 |
DLG1 |
GSK3B |
LCP2 |
MAPK9 |
NFKBIB |
PDCD1 |
PPP3CA |
RASGRP1 |
ZAP70 |
NTC |
* gene selection may be updated based on new research and development
| Catalog No. | GK021 |
|---|---|
| Country of Manufacture | United States |
| Product Code | GQH-TRS |
| Size/Quantity | One 96-well plate, one 20ul reaction per well |
| Product Use | GQH-TRS is for research use only. It is not approved for human or animal use or for application in clinical or in vitro diagnostic procedures. |
| Storage | Store at 4°C upon receipt. For long-term storage (>1 year), store the plate at -20°C in a manual defrost freezer. |
| Shipping Info | Ambient temperature. |
|
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入驻年限：15年
