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保存条件 :-20度保存
保质期 :5-10年
英文名 : GeneQuery™ Human Basal Cell Carcinoma qPCR Array Kit
库存 :大量
供应商 :上海中乔新舟生物科技有限公司
CAS号 :咨询销售
Sciencell公司产品由上海中乔新舟生物科技有限公司优质供应
美国ScienCell研究实验室(www.sciencellonline.com)成立于1999年,公司总部位于美国加州的圣地亚哥。主要致力
于实验室科研用原代细胞、原代细胞专用培养基、原代细胞无血清培养基、干细胞、干细胞培养基、干细胞无血清培养
基的研究和开发,在全球拥有众多客户。在国内销售15年来,很多老师应用其产品发表了高质量的SCI文章,且有着极高
的文献引用率。凭借着严格的质控和优秀的产品品质,深受广大科研工作者的信赖。
ScienCell研究实验室生产的原代细胞、原代细胞专用培养基都经过了严格的质量控制,细胞纯度可达98%。其中包括21
种人体正常细胞系统,90多种不同细胞类型。大多数细胞在全球唯有ScienCell实验室能够成功分离,产品质量过硬。
确保了实验结果的重复性和连贯性。
请登录Sciencell公司官方网站(www.zqxzbio.com或www.sciencellonline.com)以确保购买正规公司产品
人基底细胞癌定量PCR芯片试剂盒描述:
ScienCell's GeneQuery™ Human Basal Cell Carcinoma qPCR Array Kit (GQH-BCC) is designed to facilitate gene expression profiling of key genes involved in (i) basal cell carcinoma (BCC) biology and carcinogenesis; (ii) targeted BCC therapies; and (iii) hedgehog signaling pathway. 88 genes are selected in this kit based on database and literature research.
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery™ qPCR Array Kit Controls
Each GeneQuery™ plate contains eight controls (Figure 1).
- Five target housekeeping genes (β-actin, GAPDH, LDHA, NONO, and PPIH), which enable normalization of data.
- The Genomic DNA (gDNA) Control (GDC) detects possible gDNA contamination in the cDNA samples. It contains a primer set targeting a non-transcribed region of the genome.
- Positive PCR Control (PPC) tests whether samples contain inhibitors or other factors that may negatively affect gene expression results. The PPC consists of a predispensed synthetic DNA template and a primer set that can amplify it. The sequence of the DNA template is not present in the human genome, and thus tests the efficiency of the polymerase chain reaction itself.
- The No Template Control (NTC) is strongly recommended, and can be used to monitor the DNA contamination introduced during the workflow such as reagents, tips, and the lab bench.
GeneQuery™ Human Basal Cell Carcinoma qPCR Array Plate Layout* (8 controls in Bold and Italic)
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
A |
APC |
CD151 |
CSNK1G2 |
EIF4E |
FZD5 |
GSK3B |
LRP2 |
RAB23 |
TCF7L1 |
WNT11 |
WNT5B |
β-actin |
B |
APC2 |
CDH1 |
CSNK1G3 |
FAT2 |
FZD6 |
HHATL |
MC1R |
ROR2 |
TCF7L2 |
WNT16 |
WNT7A |
GAPDH |
C |
AXIN1 |
CEBPA |
CTNNB1 |
FBXW11 |
FZD7 |
HHIP |
NET1 |
SHFM1 |
TGFBR2 |
WNT2 |
WNT7B |
LDHA |
D |
AXIN2 |
CSNK1A1 |
DHH |
FZD1 |
FZD8 |
IFNA1 |
PARP1 |
SHH |
TP53 |
WNT2B |
WNT8A |
NONO |
E |
BMP2 |
CSNK1A1L |
DVL1 |
FZD10 |
FZD9 |
IHH |
PRKACA |
SMO |
TXN |
WNT3 |
WNT8B |
PPIH |
F |
BMP4 |
CSNK1D |
DVL2 |
FZD2 |
GAS1 |
KLF4 |
PRKACB |
STAT2 |
WNT1 |
WNT3A |
WNT9A |
GDC |
G |
BTRC |
CSNK1E |
DVL3 |
FZD3 |
GLI2 |
LEF1 |
PRKACG |
SUFU |
WNT10A |
WNT4 |
WNT9B |
PPC |
H |
CASP3 |
CSNK1G1 |
EGF |
FZD4 |
GLI3 |
LOC400927 |
PTCH1 |
TCF7 |
WNT10B |
WNT5A |
ZIC2 |
NTC |
* gene selection may be updated based on new research and development
Catalog No. | GK015 |
---|---|
Country of Manufacture | United States |
Product Code | GQH-BCC |
Size/Quantity | One 96-well plate, one 20ul reaction per well |
Product Use | GQH-BCC is for research use only. It is not approved for human or animal use, or for application in clinical or in vitro diagnostic procedures. |
Storage | Store at 4°C upon receipt. For long-term storage (>1 year), store the plate at -20°C in a manual defrost freezer. |
Shipping Info | The product is shipped at ambient temperature. Upon receipt, the plate should be stored at 4°C and is good for up to 12 months. For long-term storage (>1 year), store the plate at -20°C in a manual defrost freezer. |
References | [1] Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, Speleman F. (2002) "Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes." Genome Biol. 3(7): 1-12. |
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