HB-EGF

保存条件 ：冷藏/冷冻

保质期 ：-20°C

库存 ：大量

供应商 ：上海经科化学科技有限公司

CAS号 ：无

英文名 ：Proheparin-Binding EGF-like Growth Factor Mouse Recombinant

规格 ：10ug

重组小鼠肝素结合表皮生长因子样生长因子(HB-EGF)相关产品：

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重组小鼠肝素结合表皮生长因子样生长因子(HB-EGF)产品说明书：

Proheparin-Binding EGF-like Growth Factor Mouse Recombinant:

Catalogue Number	CYT-068
Synonyms	Heparin-binding EGF-like growth factor, DTR, HEGFL, diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor), DTSF, proheparin-binding epidermal growth factor-like growth factor.
Introduction	HB-EGF is an EGF related growth factor which signals via the EGF receptor, and stimulates the proliferation of SMC (smooth muscle cells), fibroblasts, epithelial cells and keratinocytes. HB-EGF is expressed in various cell types and tissues, including vascular endothelial cells and SMC, macrophages, skeletal muscle, keratinocytes and particular tumor cells. HB-EGF’s ability to explicitly bind heparin and heparin sulfate proteoglycans is dissimilar from other EGF-like molecules, and might be related to the enhanced mitogenic activity, relative to EGF, that HB-EGF exerts on smooth muscle cells.
Description	HB-EGF Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 86 amino acids (63-148 a.a.) and having a molecular mass of 9.8 kDa. The HB-EGF is purified by proprietary chromatographic techniques
Source	Escherichia Coli.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	The protein was filtered (0.2µm) and lyophilized from a concentrated solution containing 10mM PB and 500mM NaCl, pH7.4.
Solubility	It is recommended to reconstitute the lyophilized Mouse HB-EGF in sterile 18M-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Stability	Lyophilized Mouse HB-EGF Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution HB-EGF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Purity	Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Amino acid sequence	DLEGTDLNLF KVAFSSKPQG LATPSKERNG KKKKKGKGLG KKRDPCLRKY KDYCIHGECR YLQEFRTPSC KCLPGYHGHR CHGLTL.
Biological Activity	The ED50 was determined by a cell proliferation assay using balb/c 3T3 cells is < 1.0 ng/ml, corresponding to a specific activity of > 1.0×106 units/mg.

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