产品详情
文献和实验
相关推荐
保存条件 ：冷藏/冷冻
保质期 ：-18°C
库存 ：大量
供应商 ：上海经科化学科技有限公司
CAS号 ：无
英文名 ：Leukemia Inhibitory Factor Receptor Alpha Human Recombinant
规格 ：2ug
重组人白血病抑制因子(LIF)相关产品：Leukemia Inhibitory Factor Human Recombinant
Leukemia Inhibitory Factor, GST tag Human Recombinant
Leukemia Inhibitory Factor Human Recombinant, HEK
LIF Human Recombinant, Yeast
Leukemia Inhibitory Factor Receptor Alpha Human Recombinant
Leukemia Inhibitory Factor Mouse Recombinant
Leukemia Inhibitory Factor Rat Recombinant
重组人白血病抑制因子(LIF)
重组人白血病抑制因子,GST(LIF)
重组人白血病抑制因子,HEK(LIF)
重组人白血病抑制因子,Yeast(LIF)
重组人白血病抑制因子受体α(LIFR)
重组小鼠白血病抑制因子(LIF)
重组大鼠白血病抑制因子(LIF)
重组人白血病抑制因子(LIF)相关产品说明书：
Leukemia Inhibitory Factor Receptor Alpha Human Recombinant说明书：
| Catalogue Number | CYT-949 |
| Synonyms | Leukemia Inhibitory Factor Receptor Alpha, CD118 Antigen, LIF Receptor, LIF-R, Leukemia Inhibitory Factor Receptor, CD118, SJS2, STWS, SWS, Leukemia inhibitory factor receptor, LIF receptor, LIF-R. |
| Introduction | Leukemia inhibitory factor receptor (LIFR) is the receptor for leukemia inhibitory factor, a pleiotropic cytokine affecting the differentiation, survival, and proliferation of various cells in the adult and the embryo. LIFR plays an imperative role in a number of aspects of early pregnancy such as blastocyst implantation in the uterus. |
| Description |
LIFR produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 798 amino acids (45-833a.a.) and having a molecular mass of 90.5kDa (Molecular size on SDS-PAGE will appear at approximately 100-150kDa).
LIFR is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
|
| Source | Sf9, Baculovirus cells. |
| Physical Appearance | Sterile filtered colorless solution. |
| Formulation | LIFR protein solution (0.5mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol. |
| Stability | Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
|
| Purity | Greater than 90.0% as determined by SDS-PAGE. |
| Amino acid sequence |
ADPQKKGAPH DLKCVTNNLQ VWNCSWKAPS GTGRGTDYEV CIENRSRSCY QLEKTSIKIP ALSHGDYEIT INSLHDFGSS TSKFTLNEQN VSLIPDTPEI LNLSADFSTS TLYLKWNDRG SVFPHRSNVI WEIKVLRKES MELVKLVTHN TTLNGKDTLH HWSWASDMPL ECAIHFVEIR CYIDNLHFSG LEEWSDWSPV KNISWIPDSQ TKVFPQDKVI LVGSDITFCC VSQEKVLSAL IGHTNCPLIH LDGENVAIKI RNISVSASSG TNVVFTTEDN IFGTVIFAGY PPDTPQQLNC ETHDLKEIIC SWNPGRVTAL VGPRATSYTL VESFSGKYVR LKRAEAPTNE SYQLLFQMLP NQEIYNFTLN AHNPLGRSQS TILVNITEKV YPHTPTSFKV KDINSTAVKL SWHLPGNFAK INFLCEIEIK KSNSVQEQRN VTIKGVENSS YLVALDKLNP YTLYTFRIRC STETFWKWSK WSNKKQHLTT EASPSKGPDT WREWSSDGKN LIIYWKPLPI NEANGKILSY NVSCSSDEET QSLSEIPDPQ HKAEIRLDKN DYIISVVAKN SVGSSPPSKI ASMEIPNDDL KIEQVVGMGK GILLTWHYDP NMTCDYVIKW CNSSRSEPCL MDWRKVPSNS TETVIESDEF RPGIRYNFFL YGCRNQGYQL LRSMIGYIEE LAPIVAPNFT VEDTSADSIL VKWEDIPVEE LRGFLRGYLF YFGKGERDTS KMRVLESGRS DIKVKNITDI SQKTLRIADL QGKTSYHLVL RAYTDGGVGP EKSMYVVTKE NSHHHHHH.
|
Leukemia Inhibitory Factor Human Recombinant说明书：
| Catalogue Number | CYT-644 |
| Synonyms | CDF, HILDA, D-FACTOR, Differentiation- stimulating factor, Melanoma-derived LPL inhibitor, MLPLI, Emfilermin, Leukemia inhibitory factor, LIF, DIA. |
| Introduction | Leukemia Inhibitory Factor also called LIF is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Human and mouse LIF exhibit a 78% identity in its amino acid sequence. |
| Description | Leukemia Inhibitory Factor (LIF) Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 180 amino acids and having a molecular mass of 19.7kDa. The Leukemia Inhibitory Factor (LIF) is purified by proprietary chromatographic techniques. |
| Source | Escherichia Coli. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Leukemia Inhibitory Factor (LIF) was lyophilized from a concentrated (1mg/ml) sterile solution containing 1xPBS pH 7.4. |
| Solubility | It is recommended to reconstitute the lyophilized Leukemia Inhibitory Factor (LIF) in sterile water not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Stability | Lyophilized Leukemia Inhibitory Factor (LIF) although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Leukemia Inhibitory Factor (LIF) should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
| Purity | Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
| Amino acid sequence | SPLPITPVNA TCAIRHPCHN NLMNQIRSQL AQLNGSANAL FILYYTAQGE PFPNNLDKLC GPNVTDFPPF HANGTEKAKL VELYRIVVYL GTSLGNITRD QKILNPSALS LHSKLNATAD ILRGLLSNVL CRLCSKYHVG HVDVTYGPDT SGKDVFQKKK LGCQLLGKYK QIIAVLAQAF. |
| Biological Activity | The ED50 was determined by the M1 cell differentiation assay is < 0.01 ng/ml, corresponding to a specific activity of 100,000,000IU/mg. |
Leukemia Inhibitory Factor, GST tag Human Recombinant说明书：
| Catalogue Number | CYT-001 |
| Synonyms | D factor, MLPLI, HILDA, Emfilermin, Leukemia Inhibitory factor, Differentiation-stimulating factor, Melanoma-derived LPL inhibitor. |
| Introduction | Leukemia inhibitory factor, is a pleiotropic cytokine which is expressed by numerous cells including activated T lymphocytes, monocytes, mast cells and neuronal cells. LIF takes part in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and is a key player in immune tolerance at the maternal-fetal interface. |
| Description | LIF produced in E.Coli is a single, non-glycosylated polypeptide chain containing 415 amino acids (23-202a.a.) and having a molecular mass of 47.2kDa. LIF is fused to a 236 amino acid His-GST tag at N-terminus & purified by proprietary chromatographic techniques. |
| Source | Escherichia Coli. |
| Physical Appearance | Sterile Filtered clear solution. |
| Formulation | The LIF GST protein solution (1mg/1ml) is formulated in 20mM Tris-HCl buffer (pH 8.0) 1mM DTT, 50mM NaCl and 20% glycerol. |
| Amino Acid Sequence | MHHHHHHMSP ILGYWKIKGL VQPTRLLLEY LEEKYEEHLY ERDEGDKWRN KKFELGLEFP NLPYYIDGDV KLTQSMAIIR YIADKHNMLG GCPKERAEIS MLEGAVLDIR YGVSRIAYSK DFETLKVDFL SKLPEMLKMF EDRLCHKTYL NGDHVTHPDF MLYDALDVVL YMDPMCLDAF PKLVCFKKRI EAIPQIDKYL KSSKYIAWPL QGWQATFGGG DHPPKSDLVP RGSHMSPLPI TPVNATCAIR HPCHNNLMNQ IRSQLAQLNG SANALFILYY TAQGEPFPNN LDKLCGPNVT DFPPFHANGT EKAKLVELYR IVVYLGTSLG NITRDQKILN PSALSLHSKL NATADILRGL LSNVLCRLCS KYHVGHVDVT YGPDTSGKDV FQKKKLGCQL LGKYKQIIAV LAQAF |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Storage | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Please avoid freeze thaw cycles. |
Leukemia Inhibitory Factor Human Recombinant, HEK说明书：
| Catalogue Number | CYT-966 |
| Synonyms | CDF, HILDA, D-FACTOR, Differentiation- stimulating factor, Melanoma-derived LPL inhibitor, MLPLI, Emfilermin, Leukemia inhibitory factor, LIF, DIA. |
| Introduction | Leukemia Inhibitory Factor also called LIF is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Human and mouse LIF exhibit a 78% identity in its amino acid sequence. |
| Description | Leukemia Inhibitory Factor Human Recombinant produced in HEK cells is a glycosylated monomer, having a molecular weight of 19.9kDa due to glycosylation. The LIF is purified by proprietary chromatographic techniques. |
| Source | HEK. |
| Physical Appearence | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | The LIF protein was lyophilized from 1mg/ml in PBS pH 7.4. |
| Solubility | It is recommended to reconstitute the lyophilized LIF in sterile PBS containing 0.1% endotoxin-free recombinant HSA. |
| Stability | Lyophilized LIF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution LIF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
| Purity | Greater than 95.0% as determined by SDS-PAGE. |
| Biological Activity | The biological activity was determined by its ability to stimulate the proliferation of Human TF-1 cells, the ED50 is typically less than 0.1ng/ml. |
LIF Human Recombinant, Yeast说明书：
| Catalogue Number | CYT-191 |
| Synonyms | CDF, HILDA, D-FACTOR, Differentiation- stimulating factor, Melanoma-derived LPL inhibitor, MLPLI, Emfilermin, Leukemia inhibitory factor, LIF, DIA. |
| Introduction | Leukemia Inhibitory Factor also called LIF is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Human and mouse LIF exhibit a 78% identity in its amino acid sequence. |
| Description | LIF Human Recombinant produced in yeast is a single, glycosylated polypeptide chain containing 180 amino acids and having a molecular mass of 58.5 kDa. The LIF is purified by proprietary chromatographic techniques. |
| Source | Pichia pastoris. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | The protein was lyophilized from a 0.2 µm filtered PBS. |
| Solubility | It is recommended to reconstitute the lyophilized LIF in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Stability | Lyophilized LIF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution LIF should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles. |
| Purity | Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
| Biological Activity | The biological activity of recombinant human LIF was measured by the ability to induce differentiation of murine M1 myeloid leukemic cells. The minimal detectable concentration of human LIF in this assay is <0.05 ng/mL. The specific activity is > 1 x 108 units/mg. |
| Usage | ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
| Amino acid sequence | S P L P I T P V N A T C A I R H P C H N N L M N Q I R S Q L A Q L N G S A N A L F I L Y Y T A Q G E P F P N N L D K L C G P N V T D F P P F H A N G T E K A K L V E L Y R I V V Y L G T S L G N I T R D Q K I L N P S A L S L H S K L N A T A D I L R G L L S N V L C R L C S K Y H V G H V D V T Y G P D T S G K D V F Q K K K L G C Q L L G K Y K Q I I A V L A Q A F. |
Leukemia Inhibitory Factor Mouse Recombinant说明书：
| Catalogue Number | CYT-645 |
| Synonyms | CDF, HILDA, D-FACTOR, Differentiation- stimulating factor, Melanoma-derived LPL inhibitor, MLPLI, Emfilermin, Leukemia inhibitory factor, LIF, DIA. |
| Introduction | Leukemia Inhibitory Factor also called LIF is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Human and mouse LIF exhibit a 78% identity in its amino acid sequence. |
| Description | Leukemia Inhibitory Factor (LIF) Murine Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 181 amino acids and having a molecular mass of 20 kDa. The Leukemia Inhibitory Factor (LIF) is purified by proprietary chromatographic techniques. |
| Source | Escherichia Coli. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Leukemia Inhibitory Factor (LIF) was lyophilized from a concentrated (1mg/ml) sterile solution containing 20mM Phosphate buffer pH-7.4 and 0.02% Tween-20. |
| Solubility | It is recommended to reconstitute the lyophilized Leukemia Inhibitory Factor (LIF) in sterile water not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Stability | Lyophilized Leukemia Inhibitory Factor (LIF) although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Leukemia Inhibitory Factor (LIF) should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
| Purity | Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
| Amino acid sequence | MSPLPITPVNATCAIRHPCHGNLMNQIKNQLAQLNGSANALFISYYTAQGEPFP NNVEKLCAPNMTDFPSFHGNGTEKTKLVELYRMVAYLSASLTNITRDQKVLNP TAVSLQVKLNATIDVMRGLLSNVLCRLCNKYRVGHVDVPPVPDHSDKEAFQR KKLGCQLLGTYKQVISVVVQAF. |
| Biological Activity | Activity of murine LIF was determined by the M1 cell differentiation assay which was found to be < 0.01 ng/ml, corresponding to a specific activity of 100,000,000 IU/mg. A standard of 50 Units is defined as the concentration of mouse LIF in 1.0 mL of tissue culture medium that induces the differentiation of 50% of M1 colonies. |
Leukemia Inhibitory Factor Rat Recombinant说明书：
| Catalogue Number | CYT-731 |
| Synonyms | Leukemia inhibitory factor, Cholinergic neuronal differentiation factor, Lif. |
| Introduction | Leukemia Inhibitory Factor also called LIF is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Human and mouse LIF exhibit a 78% identity in its amino acid sequence. |
| Description | Leukemia Inhibitory Factor (LIF) Rat Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 180 amino acids and having a molecular mass of 19.8 kDa. The Leukemia Inhibitory Factor (LIF) is purified by proprietary chromatographic techniques. |
| Source | Escherichia Coli. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | LIF Rat was lyophilized from 0.2µm filtered concentrated solution in 1xPBS, pH 7.4. |
| Solubility | It is recommended to reconstitute the lyophilized Leukemia Inhibitory Factor (LIF) in sterile water not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Stability | Lyophilized Leukemia Inhibitory Factor (LIF) although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Leukemia Inhibitory Factor (LIF) should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
| Purity | Greater than 96.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
| Amino acid sequence | SPLPITPVNA TCAIRHPCHG NLMNQIKSQL AQLNGSANAL FISYYTAQGE PFPNNVDKLC APNMTDFPPF HANGTEKTKL VELYRMVTYL GASLTNITWD QKNLNPTAVS LQIKLNATTD VMRGLLSSVL CRLCNKYHVG HVDVPCVPDN SSKEAFQRKK LGCQLLGTYK QVISVLAQAF . |
| Biological Activity | The activity of rat LIF is determined by the ability to induce differentiation of M1 myeloid leukemic cells. The minimum detectable concentration of rat LIF in this assay is 0.5ng/mL. |
重组人白血病抑制因子(LIF)相关产品索引：
LIF Human
LIF Human,GST
LIF Human,HEK
LIF Human,Yeast
LIF Mouse
LIF Rat
LIFR Human
上海经科化学科技有限公司
实名认证
钻石会员
入驻年限：14年
