保存条件 ：详见说明
保质期 ：详见说明
英文名 ：Enhanced miRNA fluorescence quantitative PCR detection kit
库存 ：充足
供应商 ：杭州昊鑫生物
CAS号 ：无
规格 ：50 次x 50μl反应体系
PC49-增强型miRNA荧光定量PCR检测试剂盒
艾德莱浙江省总代理-杭州昊鑫生物
北京艾德莱生物科技有限公司致力于为生命科学研究人员提供简单、易用、值得信赖的分子生物学和生物化学试剂类产品以及专业的技术服务。同时公司针对生物试剂行业规模小，标准低，批次之间产品稳定性差的弊端，引入生物医药公司风险投资作为战略合作伙伴，将生物医药级的规模生产设备、工艺、流程等引入科研试剂领域，提供具有医药级质量标准的科研试剂。公司未来将立足生物技术前沿领域，不断推出质量稳定、价格合理的新产品，与广大科研工作者携手为中国生命科学发展不断努力。
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货号#
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规格
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价格
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PC4901
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50 次x 50μl反应体系
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490.00 元
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产品组成、储存：
-20 ℃ 避光保存至少6个月，使用前充分融解混匀。2 x miRNA qPCR Mix(With Sybr Green)短期使用可放在4 ℃，避免反复冻融。Reverse primer(10μM )每次用完置-20 ℃保存。
制品说明：
增强型miRNA荧光定量PCR检测试剂盒采用本试剂盒采用SYBR® Green I 嵌合荧光法的原理进行miRNA 荧光定量检测。本试剂盒包含miRNA 荧光定量检测的所有试剂，包括2×miRNA qPCR Mix和Reverse primer。2×miRNA qPCR Mix（含Sybr Green）是专门为miRNA 定量检测而研发的新一代预混形式的荧光定量PCR 检测试剂，其中的DNA polymerase 采用的是抗体修饰的热启动形式， 配合特殊的Buffer 体系，使反应特异性更好，灵敏度更高，并能在更广的范围内进行准确定量。
注：该试剂盒须与 miRNA 荧光定量检测试剂盒（PC4801）配套使用。
该产品被引用文献
| PC48/PC49 miRNA 加尾法反转录荧光定量PCR检测试剂盒发表文章 |
- 3,6-Dihydroxyflavone Suppresses Breast Carcinogenesis by Epigenetically Regulating miR-34a and miR-21. Cancer Prev Res 2015, 8(6)
- Expressions of miR-181a and miR-20a in RPMI8226 cell line and their potential as biomarkers for multiple myeloma.Tumor Biol 2015, DOI 10.1007/s13277-015-3600-2 , Published online:02 June 2015
- 3,6-dihydroxyflavone suppresses the epithelial-mesenchymal transition in breast cancer cells by inhibiting the Notch signaling pathway.Sci Rep. 2016; 6: 28858.Published online 2016 Jun 27. doi: 10.1038/srep28858
- Genome-Wide Analysis of microRNA Responses to the Phytohormone Abscisic Acid in populus euphratica. Frotiers in Plant Science 2016, published: 17 August 2016 doi: 10.3389/fpls.2016.01184
- Identification of phasiRNAs and their drought-responsiveness in Populus trichocarpa. FEBS letters. 2016, online: 12 September DOI: 10.1002/1873-3468.12419
- High-throughput sequencing of pituitary and hypothalamic microRNA transcriptome associated with high rate of egg production BMC Genomics 2017, 18:255
- 3,6-Dihydroxyflavone regulates microRNA-34a through DNA methylation. BMC Cancer 2017, 17:619
- MiR-181b regulates autophagy in a model of Parkinson’s disease by targeting the PTEN/Akt/mTOR signaling pathway. Neuroscience Letters 2018, 675: 83-88
- Insights into the MicroRNA-regulated response of bermudagrass to cold and salt stress. Environmental and Experimental Botany 2018, 145: 64-74
- MiR-1 inhibits prostate cancer PC3 cells proliferation through the Akt/mTOR signaling pathway by binding to c-Met.Biomedicine & Pharmacotherapy 2019, 109:1406-1410
- Expressed microRNA associated with high rate of egg production in chicken ovarian follicles.Animal Genetics 2017, 4(48): 205-216
- Vitamin D receptor activation protects against lipopolysaccharide-induced acute kidney injury through suppression of tubular cell apoptosis. American Journal of Physiology-Renal Physiology 2019, https://doi.org/10.1152/ajprenal.00332.2018
- MiR-216b inhibits osteosarcoma cell proliferation, migration, and invasion by targeting Forkhead Box M1. Journal of Cellular Biochemistry 2019, 120(4): 5435-5443
- MicroRNA-146b Overexpression Promotes Human Bladder Cancer Invasion via enhancing ETS2-mediated mmp2 mRNA Transcription. Molecular Therapy: Nucleic Acid 2019, doi: https://doi.org/10.1016/j.omtn.2019.04.007.
- Identification of lncRNA‐155 encoded by MIR155HG as a novel regulator of innate immunity against influenza A virus infection. Cellular microbiology 2019, https://doi.org/10.1111/cmi.13036.
- Vitamin D/VDR signaling induces miR-27a/b expression in oral lichen planus.Sci Rep. 2020, 10: 301.
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