手机验证
充足
cloud-clone corp(优尔)
2.74-2,000pg/mL
双抗夹心
详见说明
Mus musculus (Mouse,小鼠)
详见说明
Serum, plasma and other biological fluids
48T
本试剂盒用于检测低氧诱导因子1α(HIF1a),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。
分别于定值血清及血浆样本中加入一定量的低氧诱导因子1α(HIF1a)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。
样本 | 回收率范围(%) | 平均回收率(%) |
serum(n=5) | 93-101 | 96 |
EDTA plasma(n=5) | 88-103 | 97 |
heparin plasma(n=5) | 88-95 | 92 |
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%
在定值血清及血浆样本内加入适量的低氧诱导因子1α(HIF1a),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中低氧诱导因子1α(HIF1a)含量的测定值与理论值的比率。
样本 | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 95-103% | 78-90% | 84-91% | 96-105% |
EDTA plasma(n=5) | 84-93% | 86-96% | 83-99% | 93-101% |
heparin plasma(n=5) | 92-99% | 82-101% | 93-101% | 80-99% |
经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100μL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加底物100µL,37°C孵育10分钟;
8. 读数。
将低氧诱导因子1α(HIF1a)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的低氧诱导因子1α(HIF1a)与连接于固相载体上的抗体结合,然后加入生物素化的低氧诱导因子1α(HIF1a)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入底物。加入底物后会产生辉光型光信号。发射光强度和样品中的低氧诱导因子1α(HIF1a)呈正相关。用化学发光仪测定相对光单位(RLU),计算样品浓度。
杂志 | 参考文献 |
Cancer Chemotherapy and Pharmacology | Quercetin greatly improved therapeutic index of doxorubicin against 4T1 breast cancer by its opposing effects on HIF-1α in tumor and normal cells. [PubMed: 19466611] |
European Journal of Pharmacology | Baicalin suppresses lung carcinoma and lung metastasis by SOD mimic and HIF-1α inhibition [ScienceDirect: S0014299909011352] |
PLoS ONE | Lactate Dehydrogenase 5 Expression in Non-Hodgkin Lymphoma Is Associated with the Induced Hypoxia Regulated Protein and Poor Prognosis [PubMed: PMC3781153] |
International Journal of Molecular Medicine | Selective inhibition of PKCβ2 preserves cardiac function after myocardial infarction and is associated with improved angiogenesis of ischemic myocardium in diabetic rats [Pubmed: 24002353] |
Canadian Journal of Physiology and Pharmacology | Modulation of arginine and asymmetric dimethylarginine concentrations in liver and plasma by exogenous hydrogen sulfide in LPS-induced endotoxemia [Pubmed: 24289078] |
US8569372 B2 | Method for preventing or treating a disease, disorder or condition induced by retina ischemia [US8569372] |
J Surg Res. | Effects of adrenomedullin and vascular endothelial growth factor on ischemia/reperfusion injury in skeletal muscle in rats. [Pubmed: 23816242] |
PLoS One. | HIF-1α Is Essential for Effective PMN Bacterial Killing, Antimicrobial Peptide Production and Apoptosis in Pseudomonas aeruginosa Keratitis [Plos: Source] |
Journal of Physiological Anthropology | Telomeres are elongated in rats exposed to moderate altitude [Pubmed:Pmc4088304] |
Molecular Medicine Reports | Resveratrol abrogates the effects of hypoxia on cell proliferation, invasion and EMT in osteosarcoma cells through downregulation of the HIF-1α protein. [Pubmed:25384583] |
International Journal of Pharmaceutics | The enhancement of gene silencing efficiency with chitosan-coated liposome formulations of siRNAs targeting HIF-1α and VEGF [Pubmed:25445537] |
International Journal of Molecular Medicine | Effect of combined treatment with rosuvastatin and protein kinase Cβ2 inhibitor on angiogenesis following myocardial infarction in diabetic rats [Pubmed:25524396] |
International Journal of Developmental Neuroscience | Maternal omega-3 fatty acid supplementation to a vitamin B 12 deficient diet normalizes angiogenic markers in the pup brain at birth [PubMed: 25889224] |
Eur Surg Res | Determination of the Preferred Conditions for the Isolated Perfusion of Porcine Kidneys [PubMed: 25341590] |
Sci Rep | Oxygen-induced cell migration and on-line monitoring biomarkers modulation of cervical cancers on a microfluidic system [PubMed: 25905434] |
Evidence-Based Complementary and Alternative Medicine | Metabolomic Analysis of Biochemical Changes in the Plasma of High-Fat Diet and Streptozotocin-Induced Diabetic Rats after Treatment with Isoflavones Extract of Radix Puerariae [pmc:PMC4794592] |
Biol Trace Elem Res. | Roles of Copper in Hepatocarcinogenesis via the Activation of Hypoxia-Inducible Factor-1α [Pubmed:27121973] |
Раны и раневые инфекции. | Использование гипоксического прекондиционирования для подготовки аутодермотрансплантата у больных со скомпрометированной микроциркуляцией [91] |
Biological Trace Element Research | Roles of Copper in Hepatocarcinogenesis via the Activation of Hypoxia-Inducible Factor-1α. [pubmed:27121973] |
Experimental and Therapeutic Medicine | Expression of hypoxia-inducible factor-1α in synovial fluid and articular cartilage is associated with disease severity in knee osteoarthritis [etm:13] |
Clinical Trials in Degenerative Diseases | Correlating oxidative stress-related factors with bone metabolic markers in older adult male patients exhibiting degenerative osteoporosis in the high-altitude hypoxic area of China: study protocol for a non-randomized controlled trial [issn:2542-3975] |
Annals of Clinical & Laboratory Science | R132H Mutation in IDH1 Gene is Associated with Increased Tumor HIF1-Alpha and Serum VEGF Levels in Primary Glioblastoma Multiforme [pubmed:28667042] |
Archives of Gynecology and Obstetrics | Is there any relationship between adipocytokines and angiogenesis factors to address endothelial dysfunction and platelet aggregation in untreated patients with preeclampsia? [10.1007/s00404-017-4461-2] |
EXPERIMENTAL AND THERAPEUTIC MEDICINE | Expression of hypoxia-inducible factor-1α in synovial fluid and articular cartilage is associated with disease severity in knee [pubmed:28123469] |
Medical Journal Armed Forces India | Study of acute hypoxia markers in healthy subjects: Utility in post-crash investigation [10.1016/j.mjafi.2017.04.003] |
Journal of Physiological Anthropology | Telomere elongation protects heart and lung tissue cells from fatal damage in rats exposed to severe hypoxia [Pubmed:29454386] |
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杂志 | 参考文献 |
Cancer Chemotherapy and Pharmacology | Quercetin greatly improved therapeutic index of doxorubicin against 4T1 breast cancer by its opposing effects on HIF-1α in tumor and normal cells. [PubMed: 19466611] |
European Journal of Pharmacology | Baicalin suppresses lung carcinoma and lung metastasis by SOD mimic and HIF-1α inhibition [ScienceDirect: S0014299909011352] |
PLoS ONE | Lactate Dehydrogenase 5 Expression in Non-Hodgkin Lymphoma Is Associated with the Induced Hypoxia Regulated Protein and Poor Prognosis [PubMed: PMC3781153] |
International Journal of Molecular Medicine | Selective inhibition of PKCβ2 preserves cardiac function after myocardial infarction and is associated with improved angiogenesis of ischemic myocardium in diabetic rats [Pubmed: 24002353] |
Canadian Journal of Physiology and Pharmacology | Modulation of arginine and asymmetric dimethylarginine concentrations in liver and plasma by exogenous hydrogen sulfide in LPS-induced endotoxemia [Pubmed: 24289078] |
US8569372 B2 | Method for preventing or treating a disease, disorder or condition induced by retina ischemia [US8569372] |
J Surg Res. | Effects of adrenomedullin and vascular endothelial growth factor on ischemia/reperfusion injury in skeletal muscle in rats. [Pubmed: 23816242] |
PLoS One. | HIF-1α Is Essential for Effective PMN Bacterial Killing, Antimicrobial Peptide Production and Apoptosis in Pseudomonas aeruginosa Keratitis [Plos: Source] |
Journal of Physiological Anthropology | Telomeres are elongated in rats exposed to moderate altitude [Pubmed:Pmc4088304] |
Molecular Medicine Reports | Resveratrol abrogates the effects of hypoxia on cell proliferation, invasion and EMT in osteosarcoma cells through downregulation of the HIF-1α protein. [Pubmed:25384583] |
International Journal of Pharmaceutics | The enhancement of gene silencing efficiency with chitosan-coated liposome formulations of siRNAs targeting HIF-1α and VEGF [Pubmed:25445537] |
International Journal of Molecular Medicine | Effect of combined treatment with rosuvastatin and protein kinase Cβ2 inhibitor on angiogenesis following myocardial infarction in diabetic rats [Pubmed:25524396] |
International Journal of Developmental Neuroscience | Maternal omega-3 fatty acid supplementation to a vitamin B 12 deficient diet normalizes angiogenic markers in the pup brain at birth [PubMed: 25889224] |
Eur Surg Res | Determination of the Preferred Conditions for the Isolated Perfusion of Porcine Kidneys [PubMed: 25341590] |
Sci Rep | Oxygen-induced cell migration and on-line monitoring biomarkers modulation of cervical cancers on a microfluidic system [PubMed: 25905434] |
Evidence-Based Complementary and Alternative Medicine | Metabolomic Analysis of Biochemical Changes in the Plasma of High-Fat Diet and Streptozotocin-Induced Diabetic Rats after Treatment with Isoflavones Extract of Radix Puerariae [pmc:PMC4794592] |
Biol Trace Elem Res. | Roles of Copper in Hepatocarcinogenesis via the Activation of Hypoxia-Inducible Factor-1α [Pubmed:27121973] |
Раны и раневые инфекции. | Использование гипоксического прекондиционирования для подготовки аутодермотрансплантата у больных со скомпрометированной микроциркуляцией [91] |
Biological Trace Element Research | Roles of Copper in Hepatocarcinogenesis via the Activation of Hypoxia-Inducible Factor-1α. [pubmed:27121973] |
Experimental and Therapeutic Medicine | Expression of hypoxia-inducible factor-1α in synovial fluid and articular cartilage is associated with disease severity in knee osteoarthritis [etm:13] |
Clinical Trials in Degenerative Diseases | Correlating oxidative stress-related factors with bone metabolic markers in older adult male patients exhibiting degenerative osteoporosis in the high-altitude hypoxic area of China: study protocol for a non-randomized controlled trial [issn:2542-3975] |
Annals of Clinical & Laboratory Science | R132H Mutation in IDH1 Gene is Associated with Increased Tumor HIF1-Alpha and Serum VEGF Levels in Primary Glioblastoma Multiforme [pubmed:28667042] |
Archives of Gynecology and Obstetrics | Is there any relationship between adipocytokines and angiogenesis factors to address endothelial dysfunction and platelet aggregation in untreated patients with preeclampsia? [10.1007/s00404-017-4461-2] |
EXPERIMENTAL AND THERAPEUTIC MEDICINE | Expression of hypoxia-inducible factor-1α in synovial fluid and articular cartilage is associated with disease severity in knee [pubmed:28123469] |
Medical Journal Armed Forces India | Study of acute hypoxia markers in healthy subjects: Utility in post-crash investigation [10.1016/j.mjafi.2017.04.003] |
Journal of Physiological Anthropology | Telomere elongation protects heart and lung tissue cells from fatal damage in rats exposed to severe hypoxia [Pubmed:29454386] |