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库存 :充足
供应商 :杭州昊鑫生物
检测限 :15.6-1,000pg/mL
检测方法 :双抗夹心
应用 :详见说明
适应物种 :Sus scrofa; Porcine (Pig,猪)
标记物 :详见说明
样本 :serum, plasma, tissue homogenates and other biological fluids
规格 :48T
低氧诱导因子1α(HIF1a)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Hypoxia Inducible Factor 1 Alpha (HIF1a)
HIF1-A; MOP1; PASD8; Basic Helix-Loop-Helix Transcription Factor; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78
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优尔生位于武汉出口加工区,已整体通过ISO 9001:2008、ISO 13485:2003质量管理体系认证,以提供生命科学科研用检测试剂为主,75%以上的服务对象位于欧美国家。凭借着深厚的技术积累和庞大的引物、cDNA、质粒、杂交瘤细胞“种子库”和检测试剂半成品库库存,能够做到约11,000种蛋白、19,000种抗体以及7,000种检测试剂盒立等可取。蛋白项目部拥有四大技术平台,包括多肽合成平台、小分子抗原性改造平台、天然蛋白提取平台和采用原核(E.Coli)表达系统与真核(酵母、杆状病毒-昆虫细胞、哺乳动物细胞)表达系统的重组蛋白表达平台。抗体项目部拥有完备的单克隆抗体和多克隆抗体制备平台。免疫应用部负责检测试剂盒的研制,主要是ELISA和CLIA试剂盒。成品检测部负责所有蛋白、抗体以及检测试剂盒产成品的检测及质量控制。公司实行三级质量控制,包括原料检测、半成品检测及成品检测。
- 编号SEA798Po
- 物种Sus scrofa; Porcine (Pig,猪) 相同的名称,不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围15.6-1,000pg/mL
- 灵敏度最小可检测剂量小于等于6.4pg/mL.
- 样本类型serum, plasma, tissue homogenates and other biological fluids
- 下载英文说明书 中文说明书
- 规格48T96T 96T*5 96T*10 96T*100
- 价格¥ 3175
产品包装图模拟
特异性
本试剂盒用于检测低氧诱导因子1α(HIF1a),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的低氧诱导因子1α(HIF1a)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。
样本 | 回收率范围(%) | 平均回收率(%) |
serum(n=5) | 91-101 | 96 |
EDTA plasma(n=5) | 84-99 | 90 |
heparin plasma(n=5) | 88-101 | 94 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%
线性
在定值血清及血浆样本内加入适量的低氧诱导因子1α(HIF1a),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中低氧诱导因子1α(HIF1a)含量的测定值与理论值的比率。
样本 | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 87-97% | 78-103% | 89-97% | 91-98% |
EDTA plasma(n=5) | 94-102% | 79-101% | 86-94% | 88-98% |
heparin plasma(n=5) | 81-102% | 82-97% | 79-102% | 78-102% |
稳定性
经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100µL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分钟;
8. 加终止液50µL,立即450nm读数。
实验原理
将低氧诱导因子1α(HIF1a)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的低氧诱导因子1α(HIF1a)与连接于固相载体上的抗体结合,然后加入生物素化的低氧诱导因子1α(HIF1a)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的低氧诱导因子1α(HIF1a)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。
参考文献
杂志 | 参考文献 |
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