库存 :200
供应商 :Aidlab
规格 :10次
CV12-Seamless Assembly and Cloning kit 无缝克隆(单/多片段连接)
产品介绍:
本制品不依赖于T4连接酶,不受载体和目的片段的酶切位点限制,而直接用同源重组的方法,采用特殊的重组酶可以将任意方法线性化后的载体和与其两端具有 15-25bp 重叠区域的PCR片段(平端)定向重组,可以快速实现 1-5 个片段的高效无缝克隆。
产品储存:
储存:-20℃ 保存,避免反复冻融。
产品特点:
1.30分钟可以将一个或者多个长、短PCR扩增片段(平端)定向插入载体。
2.不受载体和插入片段酶切位点的可用性和平端/粘性末端的限制,可以在任意位点进行克隆。
3.无缝克隆,插入点不会引入不需要的碱基序列。
4.高效、准确,阳性率>95% 。
使用该产品发表的部分文章:http://www.aidlab.cn/news-show.asp?id=202
该产品被引用文献
CV12/CV19 Seamless Assembly and Cloning kit (无缝克隆试剂盒)发表文章 点击次数:2829次 |
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- A GFP Reporter Protein Combined with Flow Cytometry for Analyzing Protein-protein Interactions at the Single-bacterium Level.Journal of Xiamen University(Natural Science) 2018, 57(5): 602-609
- Overexpression of VaWRKY12, a transcription factor from Vitis amurensis with increased nuclear localization under low temperature enhances cold tolerance of plants. Plant Molecular Biology 2019, https://doi.org/10.1007/s11103-019-00846-6
- The bifunctional identification of both lycopene β- and ε-cyclases from the lutein-rich Dunaliella bardawil. Enzyme and Microbial Technology 2019, https://doi.org/10.1016/j.enzmictec.2019.109426
- Genetic Dissection of Seed Storability and Validation of Candidate Gene Associated with Antioxidant Capability in Rice (Oryza sativa L.). Int. J. Mol. Sci. 2019, 20(18), 4442
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