¥770 - 2500
丁香通研选 科研人放心采购研选大鼠肿瘤坏死因子alpha试剂盒Rat TNF-alpha ELISA Kit/Rat TNF-α ELISA Kit (大鼠肿瘤坏死因子α ELISA试剂盒)
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文献和实验
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库存 :99
供应商 :联科生物
检测限 :4.69 pg/mL - 300 pg/mL
检测方法 :双抗夹心法
应用 :ELISA
适应物种 :Rat
标记物 :NoData
样本 :血清、血浆、细胞上清
规格 :96T/48T/24T
背景介绍 | 肿瘤坏死因子α(TNF-α)也称为恶病质素和TNFSF1A,是一种脂肪因子,参与全身的炎症,同时也是刺激急性期反应的细胞因子之一。它主要由活化的巨噬细胞产生,也可由其它类型的细胞分泌,如CD4+淋巴细胞、NK细胞、中性粒细胞、肥大细胞、嗜酸性粒细胞和神经元等。它在免疫应答中具有多种调节功能,还可作为潜在的热原。TNF-α对刺激物(感染因子或组织受损)产生应答后,在全身循环,激活中性粒细胞,改变血管内皮细胞的特性,调控其它组织的代谢活性,以及通过诱导局部凝血作用展现肿瘤杀伤活性。TNF-α在关节组织和其它组织发生炎症的发病机制具有重要作用。最近,越来越多的信息表明TNF-α也参与了AIDS的发病机制。TNF-α的测定对于移植研究也非常有用。 |
检测原理 | 本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗大鼠TNF-α抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的TNF-α与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中TNF-α的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570-630 nm)测定吸光度值。 |
组分 | 包被抗TNF-α单克隆抗体的96孔聚苯乙烯酶标板 大鼠TNF-α冻干标准品 TNF-α检测抗体 标准品稀释液 检测缓冲液(10×) 底物(TMB) 终止液 洗液(20×) 封板膜 |
分析类型 | 夹心ELISA |
样本类型 | 血清、血浆,细胞培养上清及其他生物学样本 |
板式 | 96孔板,可拆 |
保存 | 试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。 |
运输条件 | 4℃蓝冰运输 |
样本体积 | 10 μl |
灵敏度 | 0.43 pg/ml |
标曲图 | |
标准曲线范围 | 4.69-300 pg/ml |
回收率范围 | 82 %-116 % |
平均回收率 | 0.96 |
板间变异系数 | 6.1 %-8.4 % |
板内变异系数 | 4.4 %-6.3 % |
大鼠肿瘤坏死因子α试剂盒说明书
本产品文献引用21篇,代表文献如下:
【1】Xiao-Juan Li, Qing-Yu Ma, You-Ming Jiang, et al. Xiaoyaosan exerts anxiolytic-like effects by down-regulating the TNF-αJAK2-STAT3 pathway in the rat hippocampus[J]. Scientific reports, 2017, 7: 353 (IF:4.259)
【2】Jinjun Shan, Linxiu Peng, Wenjuan Qian, et al. Integrated Serum and Fecal Metabolomics Study of Collagen-Induced Arthritis Rats and the Therapeutic Effects of the Zushima Tablet[J]. Pharmacol, 2018 (IF:3.978)
【3】Pei Cui, Haiming Xin, Yongming Yao, et al. Human amnion-derived mesenchymal stem cells alleviate lung injury induced by white smoke inhalation in rats[J]. Stem Cell Research & Therapy, 2018, 9: 101 (IF:4.963)
【4】Yan Liu,Ang Li, Xiuli Feng, et al. Pharmacological Investigation of the Anti-Inflammation and Anti-Oxidation Activities of Diallyl Disulfide in a Rat Emphysema Model Induced by Cigarette Smoke Extract[J]. Nutrients, 2018, 10: 79 (IF:4.171)
【5】Jiang Hua, Li Fei, Liu Shuang, et al. Indoleamine 2,3-dioxygenase is upregulated in the brain of rats with acute pancreatitis[J]. Pancreatology, 2015, 15(3): 281-285 (IF:2.837)
TNF 分子靶点信息概述
- 分子名:TNF, tumor necrosis factor
- 基因家族:Tumor necrosis factor superfamily
- 别名:TNFSF2; DIF; TNF-alpha
- 曾用名:TNFA
- 全称:TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2)
TNF 分子靶点综述
TNF-α 为单核细胞、巨噬细胞、中性粒细胞、NK 细胞和 CD4+T 细胞产生的 17 kDa 细胞因子。它在免疫应答中具有多种调节功能,还可作为潜在的热原。TNF-α对刺激物(感染因子或组织受损)产生应答后,在全身循环,激活中性粒细胞,改变血管内皮细胞的特性,调控其它组织的代谢活性,以及通过诱导局部凝血作用展现肿瘤杀伤活性。TNF-α 在关节组织和其它组织发生炎症的发病机制具有重要作用。
大鼠 Rat Tnf 分子靶点信息
- 分子名:Tnf, tumor necrosis factor
- 别称:
- cachectin
- LOC103694380
- MGC124630
- RATTNF
- TNF-a
- TNF-alpha
- Tnfa
- tumor necrosis factor (TNF superfamily, member 2)
- tumor necrosis factor alpha
- tumor necrosis factor ligand superfamily member 2
- tumor necrosis factor superfamily member 2
- tumor necrosis factor superfamily, member 2
- tumor necrosis factor-alpha
- tumor necrosis factor-like
- 基因序列:NCBI_Gene: 24835
- 蛋白序列:UniProtKB: P16599
大鼠 Rat Tnf 靶点分子功能(预测)
Enables tumor necrosis factor receptor binding activity. Involved in several processes, including positive regulation of cell communication; positive regulation of macromolecule metabolic process; and positive regulation of neuron death. Located in several cellular components, including external side of plasma membrane; extracellular space; and neuronal cell body. Used to study several diseases, including cerebrovascular disease (multiple); liver disease (multiple); peptic esophagitis; perinatal necrotizing enterocolitis; and periventricular leukomalacia. Biomarker of several diseases, including brain disease (multiple); congestive heart failure (multiple); inflammatory bowel disease (multiple); lung disease (multiple); and non-alcoholic fatty liver disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); lung disease (multiple); and skin disease (multiple). Orthologous to human TNF (tumor necrosis factor).
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入驻年限:13年