研选大鼠白介素6试剂盒Rat IL-6 ELISA Kit
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库存 ：99
供应商 ：联科生物
检测限 ：31.25 pg/mL - 2000 pg/mL
检测方法 ：双抗夹心法
应用 ：ELISA
适应物种 ：Rat
标记物 ：NoData
样本 ：血清、血浆、细胞上清
规格 ：96T/48T/24T
| 背景介绍 | 白细胞介素6(IL-6)是一种同时具备促炎性和抗炎性的细胞因子。在人类中，它由IL-6基因编码。在感染期间和受到外伤后，尤其是烧伤或其它组织损伤导致的炎症，IL-6会由T细胞和巨噬细胞分泌，促进免疫应答。研究表明，小鼠抵抗肺炎链球菌感染时需要IL-6的参与，说明IL-6在抵抗感染中发挥作用。 IL-6在很多疾病如糖尿病、动脉粥样硬化、抑郁症、阿尔茨海默氏病、系统性红斑狼疮、多发性骨髓瘤、前列腺癌、白塞氏病和类风湿性关节炎等中可促进炎性和自身免疫疾病进程。晚期/转移性癌症患者的血液中具有较高水平的IL-6。 |
| 检测原理 | 本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗大鼠IL-6抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的IL-6与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IL-6的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570-630 nm)测定吸光度值。 |
| 组分 | 包被抗IL-6单克隆抗体的96孔聚苯乙烯酶标板 大鼠IL-6冻干标准品 IL-6检测抗体 标准品稀释液 检测缓冲液(10×) 底物(TMB) 终止液 洗液(20×) 封板膜 |
| 分析类型 | 夹心ELISA |
| 样本类型 | 血清、血浆，细胞培养上清及其他生物学样本 |
| 板式 | 96孔板，可拆 |
| 保存 | 试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。 |
| 运输条件 | 4℃蓝冰运输 |
| 样本体积 | 20 μl |
| 灵敏度 | 2.57 pg/ml |
| 标曲图 |  |
| 标准曲线范围 | 31.25-2000 pg/ml |
| 回收率范围 | 92 %-104 % |
| 平均回收率 | 0.97 |
| 板间变异系数 | 6.3 %-9.1 % |
| 板内变异系数 | 4.2 %-7.7 % |
大鼠白介素6 ELISA试剂盒说明书
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- IF = 4.631 AMPK/SIRT1 Deficiency Drives Adjuvant-Induced Arthritis in Rats by Promoting Glycolysis-Mediated Monocytes Inflammatory Polarization. Journal of Inflammation Research (2022)
- IF = 4.627 Treatment with adipose tissue-derived mesenchymal stem cells exerts anti-diabetic effects, improves long-term complications, and attenuates inflammation in type 2 diabetic rats. Stem Cell Research & Therapy (2019)
- IF = 4.545 Puerarin improves the bone micro-environment to inhibit OVX-induced osteoporosis via modulating SCFAs released by the gut microbiota and repairing intestinal mucosal integrity. BIOMEDICINE & PHARMACOTHERAPY (2020)
- IF = 4.5 Puerarin Attenuates High-Glucose and High-Lipid-Induced Inflammatory Injury in H9c2 Cardiomyocytes via CAV3 Protein Upregulation. Journal of Inflammation Research (2023)
- IF = 4.486 SHP-1 suppresses endotoxin-induced uveitis by inhibiting the TAK1/JNK pathway. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2020)
- IF = 4.471 In Situ Forming Injectable Hydrogel For Encapsulation Of Nanoiguratimod And Sustained Release Of Therapeutics. International Journal of Nanomedicine (2019)
- IF = 4.451 Bovine lactoferricin ameliorates intestinal inflammation and mucosal barrier lesions in colitis through NF-κB/NLRP3 signaling pathways. Journal of Functional Foods (2022)
- IF = 4.451 The effect of Metapanax delavayi leaf extract on testosterone‐induced benign prostatic hyperplasia in rats. Journal of Functional Foods (2020)
- IF = 4.451 Inhibition of Toll-Like Receptor 4 Signaling Pathway Accelerates the Repair of Avascular Necrosis of Femoral Epiphysis through Regulating Macrophage Polarization in Perthes Disease. Tissue Engineering and Regenerative Medicine (2023)
- IF = 4.414 1H NMR Spectroscopy-Based Metabolomics Approach to Study the Anti-Stroke Activity of G-3702, a Novel Better Alternative to DL-3-n-Butylphthalide. NEUROCHEMICAL RESEARCH (2022)
- IF = 4.374 Pirfenidone inhibits epithelial-mesenchymal transition and pulmonary fibrosis in the rat silicosis model. TOXICOLOGY LETTERS (2018)
- IF = 4.36 Effect of Shixiao San on inflammatory factors and pain in rats with endometriosis. JOURNAL OF ETHNOPHARMACOLOGY (2022)
- IF = 4.36 Metabolomics combined with network pharmacology to study the mechanism of Shentong Zhuyu decoction in the treatment of rheumatoid arthritis. JOURNAL OF ETHNOPHARMACOLOGY (2021)
- IF = 4.36 Yanning Syrup ameliorates the lipopolysaccharide-induced inflammation: Adjusting the gut microbiota, short-chain fatty acids, and the CD4+ T cell balance. JOURNAL OF ETHNOPHARMACOLOGY (2021)
- IF = 4.36 Metabolomics of Spleen-Yang deficiency syndrome and the therapeutic effect of Fuzi Lizhong pill on regulating endogenous metabolism. JOURNAL OF ETHNOPHARMACOLOGY (2021)
- IF = 4.36 Tibetan medicine Ershiwuwei Lvxue Pill attenuates collagen-induced arthritis via inhibition of JAK2/STAT3 signaling pathway. JOURNAL OF ETHNOPHARMACOLOGY (2021)
- IF = 4.36 Anti-hyperglycemic and anti-hyperlipidemic effects of a special fraction of Luohanguo extract on obese T2DM rats. JOURNAL OF ETHNOPHARMACOLOGY (2019)
- IF = 4.225 Tetramethylpyrazine Ameliorates Lipopolysaccharide-Induced Sepsis in Rats via Protecting Blood–Brain Barrier, Impairing Inflammation and Nitrous Oxide Systems. Frontiers in Pharmacology (2020)
- IF = 4.225 The Anti-Depressive Effects of Hesperidin and the Relative Mechanisms Based on the NLRP3 Inflammatory Signaling Pathway. Frontiers in Pharmacology (2020)
- IF = 4.225 Low-Intensity Pulsed Ultrasound Alleviates Hypoxia-Induced Chondrocyte Damage in Temporomandibular Disorders by Modulating the Hypoxia-Inducible Factor Pathway. Frontiers in Pharmacology (2020)
- IF = 4.196 Pharmacological Investigation of the Anti-Inflammation and Anti-Oxidation Activities of Diallyl Disulfide in a Rat Emphysema Model Induced by Cigarette Smoke Extract. Nutrients (2018)
- IF = 4.162 Orally Deliverable Dual-Targeted Pellets for the Synergistic Treatment of Ulcerative Colitis. Drug Design Development and Therapy (2021)
- IF = 4.162 Qing-Luo-Yin Alleviated Monocytes/Macrophages-Mediated Inflammation in Rats with Adjuvant-Induced Arthritis by Disrupting Their Interaction with (Pre)-Adipocytes Through PPAR-γ Signaling. Drug Design Development and Therapy (2021)
- IF = 4.162 Magnesium Isoglycyrrhizinate Alleviates Arsenic Trioxide-Induced Cardiotoxicity: Contribution of Nrf2 and TLR4/NF-κB Signaling Pathway. Drug Design Development and Therapy (2021)
- IF = 4.162 Baicalin Rescues Cognitive Dysfunction, Mitigates Neurodegeneration, and Exerts Anti-Epileptic Effects Through Activating TLR4/MYD88/Caspase-3 Pathway in Rats. Drug Design Development and Therapy (2021)
- IF = 4.146 Mechanism of Incisional Pain: Novel Finding on Long Noncoding RNA XIST/miR-340-5p/RAB1A Axis:. ASN Neuro (2021)
- IF = 4.125 Folic acid supplementation in pregnant women with hepatitis B surface antigen improves infant hepatitis B surface antibody mediated by infant interleukin-4. BRITISH JOURNAL OF NUTRITION (2022)
- IF = 4.119 The therapeutic effects of Prunella vulgaris against fluoride-induced oxidative damage by using the metabolomics method. ENVIRONMENTAL TOXICOLOGY (2021)
- IF = 4.092 Protective Effects of 18β-Glycyrrhetinic Acid on Neonatal Rats with Hyperoxia Exposure. INFLAMMATION (2022)
- IF = 4.092 MicroRNA-181b Inhibits Inflammatory Response and Reduces Myocardial Injury in Sepsis by Downregulating HMGB1. INFLAMMATION (2021)
- IF = 4.079 Paeonol attenuates ligation-induced periodontitis in rats by inhibiting osteoclastogenesis via regulating Nrf2/NF-κB/NFATc1 signaling pathway. BIOCHIMIE (2018)
- IF = 4.061 Therapeutic Potential of Liraglutide for Diabetes–Periodontitis Comorbidity: Killing Two Birds with One Stone. Journal of Diabetes Research (2022)
- IF = 4.061 San-Huang-Yi-Shen Capsule Ameliorates Diabetic Kidney Disease through Inducing PINK1/Parkin-Mediated Mitophagy and Inhibiting the Activation of NLRP3 Signaling Pathway.. Journal of Diabetes Research (2022)
- IF = 4.003 Long Non-coding RNAMALAT1 Knockdown Alleviates Cerebral Ischemia/Reperfusion Injury of Rats Through Regulating the miR-375/PDE4D Axis. Frontiers in Neurology (2021)
- IF = 3.943 Astragaloside IV alleviates PM2.5-induced lung injury in rats by modulating TLR4/MyD88/NF-κB signalling pathway. INTERNATIONAL IMMUNOPHARMACOLOGY (2020)
- IF = 3.943 LXA4 protects against hypoxic-ischemic damage in neonatal rats by reducing the inflammatory response via the IκB/NF-κB pathway. INTERNATIONAL IMMUNOPHARMACOLOGY (2020)
- IF = 3.943 Nervilifordin F alleviates intestinal ischemia/reperfusion-induced acute lung injury via inhibiting inflammasome and mTOR pathway. INTERNATIONAL IMMUNOPHARMACOLOGY (2020)
- IF = 3.935 Metabolomics and pharmacodynamic analysis reveal the therapeutic role of Prunella vulgaris oil on intrauterine adhesion rats. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS (2021)
- IF = 3.926 Protective effects of methylprednisolone–cyclophosphamide treatment on bleomycin-induced pulmonary fibrosis. CYTOKINE (2023)
- IF = 3.915 Resveratrol alleviates early brain injury following subarachnoid hemorrhage: possible involvement of the AMPK/SIRT1/autophagy signaling pathway. BIOLOGICAL CHEMISTRY (2018)
- IF = 3.905 Downregulation of miR-892b inhibits the progression of osteoarthritis via targeting cyclin D1 and cyclin D2. EXPERIMENTAL CELL RESEARCH (2021)
- IF = 3.707 MiR-128-3p Alleviates Spinal Cord Ischemia/Reperfusion Injury Associated Neuroinflammation and Cellular Apoptosis via SP1 Suppression in Rat. Frontiers in Neuroscience (2020)
- IF = 3.693 Protective effect of active components of Eucommia ulmoides leaves on gastric ulcers in rats: Involvement of the PI3K/Akt/NF-κB pathway. JOURNAL OF FOOD SCIENCE (2022)
- IF = 3.69 Ethyl acetate extract of Tibetan medicine Rhamnella gilgitica ameliorated type II collagen-induced arthritis in rats via regulating JAK-STAT signaling pathway. JOURNAL OF ETHNOPHARMACOLOGY (2020)
- IF = 3.69 Forsythiae Fructuse water extract attenuates liver fibrosis via TLR4/MyD88/NF-κB and TGF-β/smads signaling pathways. JOURNAL OF ETHNOPHARMACOLOGY (2020)
- IF = 3.673 HDL-S1P protects endothelial function and reduces lung injury during sepsis in vivo and in vitro. INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY (2020)
- IF = 3.659 Alkaloids from piper longum protect dopaminergic neurons against inflammation-mediated damage induced by intranigral injection of lipopolysaccharide. BMC Complementary and Alternative Medicine (2016)
- IF = 3.659 Chemical profiles of the active fraction from Prinsepia utilis Royle leaves and its anti-benign prostatic hyperplasia evaluation in animal models. BMC Complementary and Alternative Medicine (2021)
- IF = 3.647 Selenium/Zinc-Enriched probiotics improve serum enzyme activity, antioxidant ability, inflammatory factors and related gene expression of Wistar rats inflated under heat stress. LIFE SCIENCES (2020)
- IF = 3.644 Guizhi Fuling Wan, Chinese Herbal Medicine, Ameliorates Insulin Sensitivity in PCOS Model Rats With Insulin Resistance via Remodeling Intestinal Homeostasis. Frontiers in Endocrinology (2020)
- IF = 3.584 Paeoniflorin attenuates early brain injury through reducing oxidative stress and neuronal apoptosis after subarachnoid hemorrhage in rats. METABOLIC BRAIN DISEASE (2020)
- IF = 3.575 Chronic microglial inflammation promotes neuronal lactate supply but impairs its utilization in primary rat astrocyte-neuron co-cultures. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2022)
- IF = 3.575 Fibroblast growth factor 21 attenuates hypoxia-induced pulmonary hypertension by upregulating PPARγ expression and suppressing inflammatory cytokine levels. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2018)
- IF = 3.571 Lipidomics of the erythrocyte membrane and network pharmacology to explore the mechanism of mangiferin from Anemarrhenae rhizoma in treating type 2 diabetes mellitus rats. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS (2023)
- IF = 3.571 Metabonomics and 16S rRNA gene sequencing to study the therapeutic mechanism of Danggui Sini decoction on collagen-induced rheumatoid arthritis rats with Cold Bi syndrome. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS (2022)
- IF = 3.532 Сardiac injury in rats with experimental posttraumatic stress disorder and mechanisms of its limitation in experimental posttraumatic stress disorder-resistant rats. JOURNAL OF APPLIED PHYSIOLOGY (2021)
- IF = 3.503 Gut microbiota and metabonomics used to explore the mechanism of Qing’e Pills in alleviating osteoporosis. PHARMACEUTICAL BIOLOGY (2022)
- IF = 3.47 Xanthones from Securidaca inappendiculata antagonized the antirheumatic effects of methotrexate in vivo by promoting its secretion into urine.. Expert Opinion on Drug Metabolism & Toxicology (2020)
- IF = 3.463 Knockdown of hsa_circ_0037658 inhibits the progression of osteoarthritis via inducing autophagy. Human Cell (2020)
- IF = 3.449 Correlation between intestinal microbiotal imbalance and 5-HT metabolism, immune inflammation in chronic unpredictable mild stress male rats. GENES BRAIN AND BEHAVIOR (2022)
- IF = 3.448 MiR-214-3p exacerbates kidney damages and inflammation induced by hyperlipidemic pancreatitis complicated with acute renal injury. LIFE SCIENCES (2019)
- IF = 3.448 Glucagon like peptide 2 has a positive impact on osteoporosis in ovariectomized rats. LIFE SCIENCES (2019)
- IF = 3.414 Low frequency pulsed electromagnetic field promotes the recovery of neurological function after spinal cord injury in rats. JOURNAL OF ORTHOPAEDIC RESEARCH (2018)
- IF = 3.411 Gastroprotective Effect of Ethanol Extracts from Bark of Magnolia officinalis on Ethanol-Induced Gastric Mucosal Damage in Rats. Biomed Research International (2021)
- IF = 3.411 Sanguinarine Attenuates Neuropathic Pain in a Rat Model of Chronic Constriction Injury. Biomed Research International (2021)
- IF = 3.337 Ferulic acid attenuates non-alcoholic steatohepatitis by reducing oxidative stress and inflammation through inhibition of the ROCK/NF-κB signaling pathways. JOURNAL OF PHARMACOLOGICAL SCIENCES (2021)
- IF = 3.282 Up-regulation of PPAR-γ involved in the therapeutic effect of icariin on cigarette smoke-induced inflammation. PULMONARY PHARMACOLOGY & THERAPEUTICS (2023)
- IF = 3.269 Suppression of NOD-like receptor protein 3 inflammasome activation and macrophage M1 polarization by hederagenin contributes to attenuation of sepsis-induced acute lung injury in rats. Bioengineered (2022)
- IF = 3.269 Timosaponin B-II alleviates osteoarthritis-related inflammation and extracellular matrix degradation through inhibition of mitogen-activated protein kinases and nuclear factor-κB pathways in vitro. Bioengineered (2022)
- IF = 3.269 Mechanisms of Dendrobium officinale polysaccharides in repairing gastric mucosal injuries based on mitogen-activated protein kinases (MAPK) signaling pathway. Bioengineered (2021)
- IF = 3.269 Application of serum exosomal hypoxia-inducible factor-1 alpha (HIF-1α) as potential circulating biomarker for bacterial peritonitis. Bioengineered (2022)
- IF = 3.269 Polygonatum sibiricum polysaccharides (PSP) improve the palmitic acid (PA)-induced inhibition of survival, inflammation, and glucose uptake in skeletal muscle cells. Bioengineered (2021)
- IF = 3.251 Analysis of the Potential Relationship between Aging and Pulmonary Fibrosis Based on Transcriptome. Life-Basel (2022)
- IF = 3.246 The Protective Effect of Sulodexide on Acute Lung Injury Induced by a Murine Model of Obstructive Jaundice. Biomed Research International (2022)
- IF = 3.242 Liraglutide regulates bone destruction and exhibits anti-inflammatory effects in periodontitis in vitro and in vivo. JOURNAL OF DENTISTRY (2020)
- IF = 3.241 Curcumin Inhibits Cigarette Smoke-induced inflammation via modulating the PPARγ-NF-κB Signaling Pathway. Food & Function (2019)
- IF = 3.234 Overexpression of Forkhead box C1 attenuates oxidative stress, inflammation and apoptosis in chronic obstructive pulmonary disease. LIFE SCIENCES (2018)
- IF = 3.234 Single Administration of Ultra-Low-Dose Lipopolysaccharide in Rat Early Pregnancy Induces TLR4 Activation in the Placenta Contributing to Preeclampsia. PLoS One (2015)
- IF = 3.223 Chinese herbal formula Fuzheng Huayu alleviates CCl4-induced liver fibrosis in rats: a transcriptomic and proteomic analysis. ACTA PHARMACOLOGICA SINICA (2017)
- IF = 3.222 Ischemic postconditioning protects against acute kidney injury after limb ischemia reperfusion by regulating HMGB1 release and autophagy. RENAL FAILURE (2023)
- IF = 3.216 Sanguinarine Attenuates Neuropathic Pain by Inhibiting P38 MAPK Activated Neuroinflammation in Rat Model. Drug Design Development and Therapy (2020)
- IF = 3.212 LncRNA H19 Inhibits the Progression of Sepsis-Induced Myocardial Injury via Regulation of the miR-93-5p/SORBS2 Axis. INFLAMMATION (2020)
- IF = 3.196 Changes within Liver Accounts for Fatty Acids Decrease in the Blood of Rats with Adjuvant-Induced Arthritis. EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY (2023)
- IF = 3.195 Dapagliflozin protects against dilated cardiomyopathy progression by targeting NLRP3 inflammasome activation. NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY (2023)
- IF = 3.188 Overexpression of Egr2 and Egr4 protects rat brains against ischemic stroke by downregulating JNK signaling pathway. BIOCHIMIE (2018)
- IF = 3.17 Protective effects of icariin on dorsal random skin flap survival: An experimental study. EUROPEAN JOURNAL OF PHARMACOLOGY (2019)
- IF = 3.167 Protective role of ergothioneine isolated from Pleurotus ostreatus against dextran sulfate sodium-induced ulcerative colitis in rat model. JOURNAL OF FOOD SCIENCE (2021)
- IF = 3.067 Physiological and histopathological effects of electroporation pulse on stomach of rats. BMC GASTROENTEROLOGY (2021)
- IF = 3.061 The effects of Saccharomyces boulardii on rat colonic hypermotility induced by repeated water avoidance stress and the potential mechanism. PeerJ (2022)
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