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丁香通研选 科研人放心采购研选小鼠白介素6试剂盒Mouse IL-6 ELISA Kit
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文献和实验
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库存 :99
供应商 :联科生物
检测限 :7.81 pg/mL - 500 pg/mL
检测方法 :双抗夹心法
应用 :ELISA
适应物种 :Mouse
标记物 :NoData
样本 :血清、血浆、细胞上清
规格 :96T
背景介绍 | 白细胞介素6(IL-6)是一种同时具备促炎性和抗炎性的细胞因子。在人类中,它由IL-6基因编码。在感染期间和受到外伤后,尤其是烧伤或其它组织损伤导致的炎症,IL-6会由T细胞和巨噬细胞分泌,促进免疫应答。研究表明,小鼠抵抗肺炎链球菌感染时需要IL-6的参与,说明IL-6在抵抗感染中发挥作用。 IL-6在很多疾病如糖尿病、动脉粥样硬化、抑郁症、阿尔茨海默氏病、系统性红斑狼疮、多发性骨髓瘤、前列腺癌、白塞氏病和类风湿性关节炎等中可促进炎性和自身免疫疾病进程。晚期/转移性癌症患者的血液中具有较高水平的IL-6。 |
检测原理 | 本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠IL-6抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的IL-6与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中IL-6的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。 |
组分 | 包被抗IL-6单克隆抗体的96孔聚苯乙烯酶标板 小鼠IL-6冻干标准品 IL-6检测抗体 标准品稀释液 检测缓冲液(10×) 底物(TMB) 终止液 洗液(20×) 封板膜 |
分析类型 | 夹心ELISA |
样本类型 | 血清、血浆,细胞培养上清及其他生物学样本 |
板式 | 96孔板,可拆 |
保存 | 试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。 |
运输条件 | 4℃蓝冰运输 |
样本体积 | 10 μl |
灵敏度 | 0.56 pg/ml |
标曲图 | |
标准曲线范围 | 7.81 - 500 pg/ml |
回收率范围 | 89 %-112 % |
平均回收率 | 1.02 |
板间变异系数 | 2.3 % - 3 % |
板内变异系数 | 2.1 % - 5.3 % |
小鼠白介素6 ELISA试剂盒说明书
本产品文献引用13篇,代表文献如下:
【1】Jun-Xia Zheng, Yu-Shui Han, Jin-Cai Wang, et al. Strigolactones: a plant phytohormone as novel anti-inflammatory agents[J]. MedChemCommun, 2018, 9: 181-188 (IF:5.197)
【2】BaidaKong, XiaWang, Bingnan, et al. 8:2 fluorotelomer alcohol inhibited proliferation and disturbed the expression of pro-inflammatory cytokines and antigen-presenting genes in murine macrophages[J]. Chemosphere, 2019, 219: 1052-1060 (IF:4.427)
【3】Lingman Maa, Lifen Qianb, Qidi Yingb, et al. I 4,a synthetic anti-diabetes agent, attenuates atherosclerosis through its lipid-lowering, anti-inflammatory and anti-apoptosis properties[J]. Molecular and Cellular Endocrinology, 2017, 440: 80-92 (IF:3.859)
【4】Deng J, Zeng L, Lai X, et al. Metformin protects against intestinal barrier dysfunction\r, via\r, AMPKα1-dependent inhibition of JNK signalling activation[J]. Journal of Cellular and Molecular Medicine, 2018, 22(1): 546-557 (IF:5.95)
【5】Li X, Ying-Fan H, Jia-Si W, et al. Semi-Mechanism-Based Pharmacodynamic Model for the Anti-Inflammatory Effect of Baicalein in LPS-Stimulated RAW264.7 Macrophages[J]. Frontiers in Pharmacology, 2018, 9: 793 (IF:3.831)
IL6 分子靶点信息概述
- 分子名:IL6, interleukin 6
- 基因家族:Interferons; Interleukins; Interleukin 6 type cytokine family
- 别名:IL-6; BSF2; HGF; HSF
- 曾用名:IFNB2
- 全称:interferon, beta 2; interleukin 6 (interferon, beta 2)
IL6 分子靶点综述
白细胞介素6(IL-6)是一种同时具备促炎性和抗炎性的细胞因子。在人类中,它由IL-6基因编码。在感染期间和受到外伤后,尤其是烧伤或其它组织损伤导致的炎症,IL-6会由T细胞和巨噬细胞分泌,促进免疫应答。研究表明,小鼠抵抗肺炎链球菌感染时需要IL-6的参与,说明IL-6在抵抗感染中发挥作用。IL-6在很多疾病如糖尿病、动脉粥样硬化、抑郁症、阿尔茨海默氏病、系统性红斑狼疮、多发性骨髓瘤、前列腺癌、白塞氏病和类风湿性关节炎等中可促进炎性和自身免疫疾病进程。晚期/转移性癌症患者的血液中具有较高水平的IL-6。
小鼠 Mouse Il6 分子靶点信息
- 分子名:Il6, interleukin 6
- 别称:
- Il-6
- 基因序列:NCBI_Gene: 16193
- 蛋白序列:
- UniprotKB: P08505
- UniprotKB: A0A0G2JFG1
- UniprotKB: A0A0G2JFT1
- UniprotKB: A0A0G2JGF4
- UniprotKB: A2RTD1
- UniprotKB: Q0PMN1
- UniprotKB: Q3U8J8
小鼠 Mouse Il6 靶点分子功能(预测)
Enables cytokine activity and interleukin-6 receptor binding activity. Involved in several processes, including animal organ development; positive regulation of macromolecule metabolic process; and regulation of peptide hormone secretion. Acts upstream of or within with a positive effect on gene expression. Acts upstream of or within several processes, including cellular response to cytokine stimulus; regulation of gene expression; and salivary gland morphogenesis. Located in external side of plasma membrane and extracellular space. Part of interleukin-6 receptor complex. Is expressed in several structures, including adrenal gland; genitourinary system; gut; liver; and thymus. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); gastrointestinal system cancer (multiple); glucose metabolism disease (multiple); and periodontal disease (multiple). Orthologous to human IL6 (interleukin 6).
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