原代肾小管上皮Renal Proximal Tubular Epithelial Cells价格

库存：99

供应商：BIOCYTO

肿瘤类型：NC

细胞类型：primary

ATCC Number ：NC

品系：NC

组织来源：KINDY

相关疾病：NC

物种来源：RAT

免疫类型：NC

细胞形态：圆梭形

是否是肿瘤细胞：否

器官来源：Renal

运输方式：常温

年限：NC

生长状态：贴壁

规格：10^6

Renal proximal tubular epithelial cells (RPTEpiC) play a crucial role in renal function. They reabsorb nearly all of the glucose and amino acids in the glomerular filtrate, while allowing other substances of no nutritional value to be excreted in the urine. They are also a major site of injury in a variety of congenital, metabolic, and inflammatory diseases. RPTEpiC can produce inflammatory mediators such as cytokines or chemokines and actively participate in acute inflammatory processes by affecting and directing leukocyte chemotaxis via the production of IL-8 [1, 2]. RPTEpiC express IL-2R alpha and MHC class II antigens during inflammation after renal transplantation or in crescentic glomerulonephritis, indicating the capacity to participate in the pathogenesis of immune renal injury [3]. To be able to study the relationship between proximal tubular cells and a variety of renal diseases, the RPTEpiC culture provides a useful in vitro model.

RRPTEpiC from ScienCell Research Laboratories are isolated from neonate day 2 CD® IGS rat kidney tissue. RRPTEpiC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. RRPTEpiC are characterized by immunofluorescent method with antibodies to cytokeratin-18, -19 and vimentin. RRPTEpiC are negative for mycoplasma, bacteria, yeast and fungi. RRPTEpiC are guaranteed to further culture under the conditions provided by ScienCell Research Laboratories; however, RRPTEpiC are not recommended for long-term cultures due to limited expansion capacity and senescence after subculturing.

Product	Catalog no.	Amount	Storage
RPTEpiC	RPT001	1X10^6/vial	in liquid nitrogen

Product Use
For Research Use Only.Not for use in diagnostic procedures

Culture Conditions
Culture Type:Adherent
Temperature Range:36℃ to 38℃
Incubator Atmosphere:Humidified atmosphere of 5% CO2

Passaging Adherent Cells
All solutions and equipment that come in contact with the cells must be sterile.
Always use proper sterile technique and work in a laminar flow hood.
1. Remove and discard the spent cell culture media from the culture vessel.
2. Wash cells using a balanced salt solution without calcium and magnesium (approximately 2 mL per 10 cm2 culture surface area). Gently add washsolution to the side of the vessel opposite the attached cell layer to avoiddisturbing the cell layer,and rock the vessel back and forth several times.
Note: The wash step removes any traces of serum, calcium, and magnesium that would inhibit the action of the dissociation reagent.
3. Remove and discard the wash solution from the culture vessel
4. Add the pre-warmed dissociation reagent such as trypsin or TrypLE™to the side of the flask; use enough reagent to cover the cell layer (approximately0.5 mL per 10 cm2). Gently rock the container to get complete coverage of the cell layer.
5. Incubate the culture vessel at room temperature for approximately 2minutes.
Note: that the actual incubation time varies with the cell line used.
6. Observe the cells under the microscope for detachment. If cells areless than 90% detached, increase the incubation time a few more minutes, checking for dissociation every 30 seconds. You may also tap the vessel to expedite cell Tetachment.
7. When ≥ 90% of the cells have detached, tilt the vessel for a minimallength of time to allow the cells to drain. Add the equivalent of 2 volumes (twice thevolume used for the dissociation reagent) of pre-warmed complete growth medium.Disperse the medium by pipetting over the cell layer surface several times.
8. Transfer the cells to a 15-mL conical tube and centrifuge then at200 × g for 5 to 10 minutes. Note that the centrifuge speed and time vary based on the cell type.
9. Resuspend the cell pellet in a minimal volume of pre-warmed complete growth medium and remove a sample for counting.
10. Determine the total number of cells and percent viability using a hemacytometer, cell counter and Trypan Blue exclusion, or the Countess® Automated CellCounter. If necessary, add growth media to the cells to achieve the desired cellconcentration and recount the cells.