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保存条件 ：Store at -20°C in a non-frost-free freezer.
英文名 ：Platinum™ Green Hot Start PCR Master Mix (2X)
库存 ：大量
供应商 ：恒斐生物
CAS号 ：相关应用： Capillary Electrophoresis Sequencing & Fragment Analysis | Genotyping & Genomic Profiling | PCR | 甲基化分析
规格 ：50 reactions
Platinum Green Hot Start PCR Master Mix (2X)抗体热启动酶
| Polymerase: | Platinum™ Taq DNA Polymerase |
|---|---|
| Hot Start: | Built-In Hot Start |
| Fidelity (vs. Taq): | 1 X |
| GC-Rich PCR Performance: | Medium |
| Reaction Format: | SuperMix or Master Mix |
| Product Overhang: | 3'-A |
| Reaction Speed: | Standard |
| Final Product Size(s): | 5 kb or less |
| Product Size: | 50 reactions |
| Shipping Condition: | Dry Ice |
Platinum Green Hot Start PCR Master Mix (2X)抗体热启动酶
内容及储存
Each box contains:
• 1.25 mL Platinum Green Hot Start PCR 2X Master Mix
• 1.25 mL Platinum GC Enhancer
• 1.25 mL water, nuclease-free
Store at -20°C in a non-frost-free freezer.
• 1.25 mL Platinum Green Hot Start PCR 2X Master Mix
• 1.25 mL Platinum GC Enhancer
• 1.25 mL water, nuclease-free
Store at -20°C in a non-frost-free freezer.
Product features:
• High specificity and increased yields with antibody-mediated hot-start PCR
• Convenient room temperature reaction setup
• Direct gel loading with green PCR master mix
• Versatile formulation for broad range of amplicons
About Platinum Taq DNA Polymerase
Platinum Taq DNA Polymerase is a recombinant Taq DNA polymerase complexed with proprietary antibodies that inhibit enzyme activity at room temperature, reducing nonspecific target amplification at lower temperatures. The polymerase is activated in a temperature-dependent manner (at 94°C) during the start of PCR. Once dissociated from the antibodies, theTaq DNA polymerase regains its full activity. Just as with Taq DNA Polymerase, Platinum TaqDNA Polymerase has a non-template-dependent terminal transferase activity that adds a 3' deoxyadenosine to product ends and has a 5'→3' exonuclease activity (but not 3'→5' exonuclease activity).
Using Platinum Green Hot Start PCR Master Mix
Convenient Platinum Green Hot Start PCR Master Mix provides a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs, thus reducing the number of pipetting steps during PCR reaction setup. All you have to do is add template, specific primers, and water. The two tracking dyes in Platinum Green Hot Start PCR Master Mix enable direct gel loading of PCR products, thus eliminating tedious steps of dye addition. The dyes in the master mix do not interfere with PCR performance and are compatible with downstream applications, including DNA sequencing, ligation, and restriction digestion. Platinum Green Hot Start PCR Master Mix is supplied with a separate vial of Platinum GC Enhancer formulated for specific amplification and improved yields of GC-rich targets. Use Platinum Green Hot Start PCR Master Mix in PCR applications such as genotyping, gene expression profiling, and next-generation sequencing.
| 货号 |
单位规格
|
单价 (CNY) | 数量 | |
|---|---|---|---|---|
| 13001012 也被称为 13001-012 |
50 reactions
|
596.00 | ||
| 13001013 也被称为 13001-013 |
200 reactions
|
2,237.00 | ||
| 13001014 也被称为 13001-014 |
1000 reactions
|
8,851.00 |
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入驻年限：12年
