RKO 人结肠腺癌细胞价格_品牌:ATCC传代-丁香通官网

库存：大量

供应商：复祥生物

细胞类型：普通细胞株-科研实验

ATCC Number ：CRL-2577

物种来源：人

运输方式：常温

生长状态：正常

ATCC^® Number:

CRL-2577™

Price:

$0.00

Designations:	RKO 人结肠腺癌
Depositors:	MC Hollander, AJ Fornace
Biosafety Level:	1
Shipped:	frozen
Medium & Serum:	See Propagation
Growth Properties:	adherent
Organism:	Homo sapiens (human)
Morphology:	epithelial
Source:	Organ: colon Disease: carcinoma
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Receptors:	urokinase receptor (u-PAR) [51462]
Tumorigenic:	Yes
Oncogene:	p53 + (wild type)
Comments:	RKO is a poorly differentiated colon carcinoma cell line developed by Michael Brattain. RKO cells contain wild-type p53 but lack endogenous human thyroid receptor nuclear receptor (h-TRbeta1). The level of p53 protein is higher in RKO (ATCC CRL-2577) cells than in RKO-E6 (ATCC CRL-2578) cells. [53331] The RKO cell line is the parental cell line (isogenic) of RKO-E6 (ATCC CRL-2578) and RKO-A545-1 (ATCC CRL-2579). It can be used as the control cell line for investigating the effects of p53 and gadd45 on cellular parameters.
Propagation:	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C
Subculturing:	Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:12 is recommended Medium Renewal: Every 2 to 3 days Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation:	culture medium 95%; DMSO, 5%
Related Products:	Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003 recommended serum:ATCC 30-2020 derivative:ATCC CRL-2578 derivative:ATCC CRL-2579
References:	51462: Boyd D, et al. Determination of the levels of urokinase and its receptor in human colon carcinoma cell lines. Cancer Res. 48: 3112-3116, 1988. PubMed: 2835152 51463: Smith ML, et al. Involvement of the p53 tumor suppressor in repair of u.v.-type DNA damage. Oncogene 10: 1053-1059, 1995. PubMed: 7700629 53329: Brattain MG, et al. Heterogeneity of human colon carcinoma. Cancer metastasis Rev. 3: 177-191, 1984. PubMed: 6437669 53331: Bhat MK, et al. Tumor suppressor p53 is a negative regulator in thyroid hormone receptor signaling pathways. J. Biol. Chem. 272: 28989-28993, 1997. PubMed: 9360971

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RKO 人结肠腺癌细胞

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