Recombinant Mouse IFN gamma (E. coli)

英文名 ：Recombinant Mouse IFN gamma (E. coli)

规格 ：10ug/50ug/500ug/1mg

Recombinant Mouse IFN gamma (E. coli) (C746)

产品说明(Description)

Recombinant Mouse Interferon Gamma is produced by our E.coli expression system and the target gene encoding His23-Cys155 is expressed.

Accession #: P01580

Known as: Ifng;Interferon gamma; IFN-gamma

制剂(Formulation)

Lyophilized from a 0.2 μm filtered solution of 4mM HCl.

质量控制(Quality Control)

Purity: Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

Bioactivity:

Determined by its ability to inhibit the proliferation of murine WEHI-279 cells. The ED50 for this effect is 1 ng/mL.	000000

复溶(Reconstitution)

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in 4mM HCl.

Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

保存(Storage)

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.

Reconstituted protein solution can be stored at 4-7°C for 2-7 days.

Aliquots of reconstituted samples are stable at < -20°C for 3 months.

背景(Background)

Mouse Ifng is a secreted protein which belongs to the type I I (or gamma) interferon family. IFNG is produced by lymphocytes and activated by specific antigens or mitogens. In addition to having antiviral activity, IFNG also has important immunoregulatory functions. It is a potent activator of macrophages and has antiproliferative effects on transformed cells. It can potentiate the antiviral and antitumor effects of the type I interferons. Genetic variation in IFNG is associated with the risk of aplastic anemia (AA) which is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.

电泳(SDS-PAGE)

参考文献(Reference)

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Human amniotic mesenchymal stem cells-conditioned medium protects mice from high-fat diet-induced obesity[J]. Hui-Lan Tan. et al. Stem Cell Research & Therapy. 2021.

Podocytes present antigen to activate specific T cell immune responses in inflammatory renal disease[J]. Shan Li. et al. JOURNAL OF PATHOLOGY. 2020.

Deficiency of Complement C3a and C5a Receptors Prevents Angiotensin II-Induced Hypertension via Regulatory T Cells[J]. Xiao Hui Chen. et al. Circulation Research. 2018.

17β-Estradiol promotes LC3B-associated phagocytosis in trained immunity of female mice against sepsis[J]. Zhiheng Sun. et al. International Journal of Biological Sciences. 2021.

Target-Triggered Quadratic Amplification for Label-Free and Sensitive Visual Detection of Cytokines Based on Hairpin Aptamer DNAzyme Probes[J]. Zhou W. et al. Analytical Chemistry. 2013.

Label-free and amplified electrochemical detection of cytokine based on hairpin aptamer and catalytic DNAzyme[J]. Haixia Zhang. et al. Cite this: Analyst. 2012.

FOR RESEARCH USE ONLY