Anti-WNT3 Antibody

价格： ￥1060 - 2580

品牌：BOSTER 品牌认证

货号：PB0656

产品详情

产品概况

货号	PB0656
产品名称	Anti-WNT3 Antibody
基因名	WNT3
抗体来源	Rabbit
克隆	Polyclonal
抗体亚型	Rabbit IgG
分子量	45KD
免疫原	A synthetic peptide corresponding to a sequence in the middle region of human Wnt3 (185-207aa AMNKHNNEAGRTTILDHMHLKCK), identical to the related mouse sequence.
内容	500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol.
纯化方式	Immunogen affinity purified.
浓度	500 ug/ml
产品形态	Liquid
保存条件	12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料	The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98% amino acid identity to mouse Wnt3 protein, and 84% to human WNT3A protein, another WNT gene product. The mouse studies show the requirement of Wnt3 in primary axis formation in the mouse. Studies of the gene expression suggest that this gene may play a key role in some cases of human breast, rectal, lung, and gastric cancer through activation of the WNT-beta-catenin-TCF signaling pathway. This gene is clustered with WNT15, another family member, in the chromosome 17q21 region.
研究类别	1. "Entrez Gene: WNT3 wingless-type MMTV integration site family, member 3".2. Liu P, Wakamiya M, Shea MJ et al. (1999). "Requirement for Wnt3 in vertebrate axis formation".Nat. Genet. 22 (4): 361–5. 3. Roelink H, Wang J, Black DM, Solomon E, Nusse R (Dec 1993). "Molecular cloning and chromosomal localization to 17q21 of the human WNT3 gene". Genomics 17 (3): 790–2.
Uniprot ID	WNT3: P56703
推荐配套的二抗和检测试剂	Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。

产品应用细节

博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。

应用	稀释度*
Western blot(WB):	1:500-2000

*最佳稀释度需要用户自己调试，此处数据仅供参考。

**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。

产品图片描述

点击图片放大

[list_product_images]Figure 1. Western blot analysis of Anti-WNT3 antibody (PB0656). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Human placenta tissue lysates,Lane 2: HEPG2 whole cell lysates,Use rabbit Anti-WNT3 1:1000, probed with a goat Anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for WNT3 at approximately 45KD. The expected band size for WNT3 is at 45KD.[/list_product_images]