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克隆性 :Polyclonal
适应物种 :human, mouse, rat
保质期 :发货周期:现货
库存 :9999
供应商 :博士德生物
应用范围 :WB
规格 :50μl/100μl/150μl
产品概况
货号 | BA0140 |
---|---|
产品名称 | Anti-MAPT/TAU Antibody |
基因名 | MAPT |
抗体来源 | Rabbit |
克隆 | Polyclonal |
抗体亚型 | Rabbit IgG |
分子量 | 50-80KD |
免疫原 | A synthetic peptide corresponding to a sequence at the N-terminus of human Tau(29-44aa YTMHQDQEGDTDAGLK), different from the related mouse and rat sequences by three amino acids. |
内容 | 500 ug/ml antibody with PBS ,0.02% NaN3 , 1 mg BSA and 50% glycerol. |
纯化方式 | Immunogen affinity purified. |
浓度 | 500 ug/ml |
产品形态 | Liquid |
保存条件 | 12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
背景资料 | MAPT, Microtubule-associated protein tau, appears to be enriched in axons. The MAPT gene is assigned to chromosome 17 by hybridization of a cDNA clone to flow-sorted and spot-blotted chromosomes and to 17q21 by in situ hybridization, containing 16 exons. The tau proteins are the product of alternative splicingfrom a single gene that in humans is designated MAPT. Tau proteins are proteins that stabilize microtubules. They are abundant in neurons in the central nervous system and are less common elsewhere. When tau proteins are defective, and no longer stabilize microtubules properly, they can result in dementias such as Alzheimer's disease. |
研究类别 | 1. Andreadis, A., Brown, W. M., Kosik, K. S.Structure and novel exons of the human tau gene.Biochemistry 31: 10626-10633, 1992.2. Donlon, T. A., Harris, P., Neve, R. L.Localization of microtubule-associated protein tau (MTBT1) to chromosome 17q21. (Abstract)Cytogenet. Cell Genet. 46: 607, 1987.3. Rademakers, R., Cruts, M., van Broeckhoven, C.The role of tau (MAPT) in frontotemporal dementia and related tauopathies.Hum. Mutat. 24: 277-295, 2004. |
Uniprot ID | MAPT: P10636 |
推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测,以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
应用 | 稀释度* |
---|---|
Western blot(WB): | 1:500-2000 |
*最佳稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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[list_product_images]Figure 1. Western blot analysis of Anti-MAPT antibody (BA0140). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Mosue brain tissue lysates,Lane 2: Mosue brain tissue lysates,Lane 3: HT1080 whole cell lysates,Lane 4: MCF-7 whole cell lysates,Use rabbit Anti-MAPT 1:1000, probed with a goat Anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for MAPT at approximately 79KD. The expected band size for MAPT is at 79KD.[/list_product_images]
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