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克隆性 ：Polyclonal
适应物种 ：human
保质期 ：发货周期：现货
库存 ：9999
供应商 ：博士德生物
应用范围 ：WB, IHC, ICC
规格 ：50μl/100μl/150μl
产品概况
| 货号 | BA3353 |
|---|---|
| 产品名称 | Anti-SERPINA6 Antibody |
| 基因名 | SERPINA6 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 60KD |
| 免疫原 | A synthetic peptide corresponding to a sequence at the N-terminus of human Cortisol Binding Globulin(102-120aa ETEIHQGFQHLHQLFAKSD). |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Corticosteroid-binding globulin(CBG), also known as Serpin A6 is a protein that in humans is encoded by the SERPINA6 gene. It is mapped 14q32.13. This gene encodes an alpha-globulin protein with corticosteroid-binding properties. This is the major transport protein for glucorticoids and progestins in the blood of most vertebrates. The gene localizes to a chromosomal region containing several closely related serine protease inhibitors which may have evolved by duplication events. |
| 研究类别 | 1. Buss, C., Schuelter, U., Hesse, J., Moser, D., Phillips, D. I., Hellhammer, D., Meyer, J. Haploinsufficiency of the SEPRINA6 gene is associated with severe muscle fatigue: a de novo mutation in corticosteroid-binding globulin deficiency. J. Neural Transm. 114: 563-569, 2007.2. Torpy, D. J., Bachmann, A. W., Grice, J. E., Fitzgerald, S. P., Phillips, P. J., Whitworth, J. A., Jackson, R. V. Familial corticosteroid-binding globulin deficiency due to a novel null mutation: association with fatigue and relative hypotension. J. Clin. Endocr. Metab. 86: 3692-3700, 2001. |
| Uniprot ID | SERPINA6: P08185 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Immunocytochemistry: | 1:50-400 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*最佳稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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[list_product_images]Figure 1. Western blot analysis of anti- SERPINA6 antibody (BA3353). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HL-60 whole cell lysates,
Lane 2: human A375 whole cell lysates,
Lane 3: human 293T whole cell lysates.
Use rabbit anti- SERPINA6 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for SERPINA6 at approximately 60KD. The expected band size for SERPINA6 is at 53KD.|Figure 2. ICC analysis of SERPINA6 using anti- SERPINA6 antibody (BA3353).
SERPINA6 was detected in an immunocytochemical section of HEPG2 cells. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.|Figure 3. IHC analysis of Cortisol Binding Globulin using anti-Cortisol Binding Globulin antibody (BA3353).
Cortisol Binding Globulin was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Cortisol Binding Globulin Antibody (BA3353) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.[/list_product_images]
Lane 1: human HL-60 whole cell lysates,
Lane 2: human A375 whole cell lysates,
Lane 3: human 293T whole cell lysates.
Use rabbit anti- SERPINA6 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for SERPINA6 at approximately 60KD. The expected band size for SERPINA6 is at 53KD.|Figure 2. ICC analysis of SERPINA6 using anti- SERPINA6 antibody (BA3353).
SERPINA6 was detected in an immunocytochemical section of HEPG2 cells. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.|Figure 3. IHC analysis of Cortisol Binding Globulin using anti-Cortisol Binding Globulin antibody (BA3353).
Cortisol Binding Globulin was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Cortisol Binding Globulin Antibody (BA3353) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.[/list_product_images]
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