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克隆性 :Polyclonal
适应物种 :human, mouse, rat
保质期 :发货周期:现货
库存 :9999
供应商 :博士德生物
应用范围 :WB
规格 :50μl/100μl/150μl
产品概况
货号 | BA3612-2 |
---|---|
产品名称 | Anti-MDM2 Antibody |
基因名 | MDM2 |
抗体来源 | Rabbit |
克隆 | Polyclonal |
抗体亚型 | Rabbit IgG |
分子量 | 90-100KD |
免疫原 | A synthetic peptide corresponding to a sequence at the N-terminus of human MDM2(103-119aa RKIYTMIYRNLVVVNQQ), different from the related mouse sequence by three amino acids, and different from rat sequence by two amino acids. |
内容 | 500 ug/ml antibody with PBS ,0.02% NaN3 , 1 mg BSA and 50% glycerol. |
纯化方式 | Immunogen affinity purified. |
浓度 | 500 ug/ml |
产品形态 | Liquid |
保存条件 | 12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
背景资料 | Mdm2 is an important negative regulator of the p53 tumor suppressor. It is the name of a gene as well as the protein encoded by that gene. Mdm2 protein functions both as an E3 ubiquity lipase that recognizes the N-terminal trans-activation domain(TAD) of the p53 tumor suppressor and an inhibitor of p53 transcriptional activation. Oliner et al.(1992) used MDM2 clones to localize the human gene to chromosome 12q13-q14 by analysis of human-hamster somatic cell hybrids. |
研究类别 | 1. Uhrinova S, Uhrin D, Powers H, et al (2005). "Structure of free MDM2 N-terminal domain reveals conformational adjustments that accompany p53-binding". J. Mol. Biol. 350 (3): 587–98. |
Uniprot ID | MDM2: Q00987 |
推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测,以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
应用 | 稀释度* |
---|---|
Western blot(WB): | 1:500-2000 |
*最佳稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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[list_product_images]Figure 1. Western blot analysis of anti- MDM2 antibody (BA3612-2). The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates, Use rabbit anti- MDM2 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for MDM2 at approximately 95KD. The expected band size for MDM2 is at 55KD.[/list_product_images]
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