产品详情
文献和实验
相关推荐
克隆性 ：Monoclonal(Clone:C3)
适应物种 ：human
保质期 ：发货周期：现货
库存 ：9999
供应商 ：博士德生物
应用范围 ：IHC
规格 ：50μl/100μl/150μl
产品概况
| 货号 | BM0014 |
|---|---|
| 产品名称 | Anti-AFP Antibody |
| 基因名 | AFP |
| 抗体来源 | Mouse |
| 克隆 | Monoclonal(Clone:C3) |
| 抗体亚型 | Mouse IgG2a |
| 免疫原 | Human alpha-fetoprotein. |
| 内容 | 200ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA and 50% glycerol. |
| 纯化方式 | Ascites |
| 浓度 | 200ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Alpha-fetoprotein(AFP) is a major plasma protein in the fetus, where it is produced by the yolk sac and liver. Direct confirmation of the assignment of the AFP gene to chromosome 4 by in situ hybridization was provided by Harper and Dugaiczyk(1983), who placed the gene in the q11-q22 region, the same region as the albumin gene. Structure and evolution of human alpha-fetoprotein deduced from partial sequence of cloned cDNA.As the major fetal serum protein, Alpha-fetoprotein is not essential for embryonic development but is required for female fertility. |
| 研究类别 | 1. D'Eustachio, P.; Ingram, R. S.; Tilghman, S. M.; Ruddle, F. H. : Murine alpha-fetoprotein and albumin: two evolutionarily linked proteins encoded on the same mouse chromosome. Somat. Cell Genet. 7: 289-294, 1981. 2. Beattie, W. G.; Dugaiczyk, A. : Structure and evolution of human alpha-fetoprotein deduced from partial sequence of cloned cDNA. Gene 20: 415-422, 1982. 3. Gabant, P.; Forrester, L.; Nichols, J.; Van Reeth, T.; De Mees, C.; Pajack, B.; Watt, A.; Smitz, J.; Alexandre, H.; Szpirer, C.; Szpirer, J. : Alpha-fetoprotein, the major fetal serum protein, is not essential for embryonic development but is required for female fertility. Proc. Nat. Acad. Sci. 99: 12865-12870, 2002. |
| Uniprot ID | AFP: P02771 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC. |
产品应用细节
博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*最佳稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
点击图片放大
[list_product_images]IHC(P): Human Liver Cancer Tissue |IHC(P): Human Hepatitis Tissue [/list_product_images]
武汉博士德生物工程有限公司
品牌商实名认证
金牌会员
入驻年限：17年
