Anti-GAPDH Antibody

价格： ￥880 - 6800

品牌：BOSTER 品牌认证

货号：BM1623

产品详情

产品概况

货号	BM1623
产品名称	Anti-GAPDH Antibody
基因名	GAPDH
抗体来源	mouse
克隆	Monoclonal(Clone:5A12)
抗体亚型	mouse IgG1
分子量	36KD
免疫原	A synthetic peptide corresponding to a sequence
内容	200ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA
纯化方式	protein G purified.
浓度	200 ug/ml
产品形态	Liquid
保存条件	12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料	The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. Two transcript variants encoding different isoforms have been found for this gene.
Uniprot ID	GAPDH: P04406

产品应用细节

博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。

应用	稀释度*
Western blot (WB):	1:10000-200000
Immunocytochemistry/Immunofluorescence(ICC/IF):	1:200-500

*最佳稀释度需要用户自己调试，此处数据仅供参考。

**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。

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[list_product_images]Figure 1. Western blot analysis of anti- GAPDH antibody (BM1623). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: monkey COS-7 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat RH-35 whole cell lysates,
Lane 7: mouse brain tissue lysates.
Use mouse anti- GAPDH 1:1000, probed with a goat anti- mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1001). A specific band was detected for GAPDH at approximately 36KD. The expected band size for GAPDH is at 36KD.[/list_product_images]