Anti-Survivin(BIRC5) Antibody

免疫原 ：0

亚型 ：0

形态 ：0

保存条件 ：0

克隆性 ：单克隆

适应物种 ：human

抗原来源 ：0

库存 ：5-7天

供应商 ：博士德生物

应用范围 ：IHC-P,IC,FC,ELISA

浓度 ：0

规格 ：200ul

产品概况

产品名称	Survivin(BIRC5) Antibody
产品描述	Anti-Survivin(BIRC5) Antibody validated for IHC, FC, ELISA in Human.
文献引用格式	Survivin(BIRC5) Antibody (Boster Biological Technology, Wuhan, China. Catalog #BM1420)
应用范围	IHC, FC, ELISA
抗体来源	Mouse
抗体亚型	IgG1
免疫原	Purified recombinant fragment of human BIRC5 expressed in E. Coli.
抗体规格	200ul/1180元

产品详情

克隆	Monoclonal
克隆号	单克隆抗体：IgG1；克隆号：1H5
产品形态	Liquid
内容	Ascitic fluid containing 0.03% sodium azide.
储存条件	连续使用时4℃存储，保质期六个月；长期存储时建议分装为10ul以上小包装-20℃存储，并避免反复冻融，保质期一年。

指标相关信息 (来源: Uniprot.org)

小贴士：你可以利用tissue specificity信息来帮助设计阳性和阴性对照。对此有疑问请联系我们技术服务部门。

基因名	BIRC5
基因全名	Baculoviral IAP repeat-containing protein 5
蛋白功能	Expressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines. .
Tissue Specificity	Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis. Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis. Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May counteract a default induction of apoptosis in G2/M phase. The acetylated form represses STAT3 transactivation of target gene promoters. May play a role in neoplasia. Inhibitor of CASP3 and CASP7. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform. .
Sequence Similarities	Baculoviral IAP repeat-containing protein 5;Apoptosis inhibitor 4;Apoptosis inhibitor survivin;BIRC5;API4, IAP4;
Uniprot ID	BIRC5: O15392

指标背景简介

This gene is a member of the inhibitor of apoptosis (IAP) gene family, which encode negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but this gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors, yet low in adult tissues. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

产品应用细节

为了提供最优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。

应用	稀释度*
Western blotting	1:100-400
Immunohistochemistry in paraffin section	1:50-200
Immunocytochemistry in fixed cells	1:50-200
Flow Cytometry	1:40-80
enzyme-linked immuno sorbent assay	1:2000

*最优稀释度需要用户自己调试，此处数据仅供参考。

**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。

产品图片

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[list_product_images]Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using BIRC5 mouse mAb with DAB staining. |Immunohistochemical analysis of paraffin-embedded colon cancer tissues using BIRC5 mouse mAb with DAB staining. |Flow cytometric analysis of Jurkat cells using BIRC5 mouse mAb (green) and negative control (red). |Black line: Control Antigen (100 ng);Purple line: Antigen(10ng);Blue line: Antigen (50 ng);Red line: Antigen (100 ng);[/list_product_images]