Anti-RAF1 Antibody

免疫原 ：0

亚型 ：0

形态 ：0

保存条件 ：0

克隆性 ：单克隆

适应物种 ：human

抗原来源 ：0

库存 ：5-7天

供应商 ：博士德生物

应用范围 ：WB,FC,ELISA

浓度 ：0

规格 ：200ul

产品概况

产品名称	RAF1 Antibody
产品描述	Anti-RAF1 Antibody validated for WB, FC, ELISA in Human.
文献引用格式	RAF1 Antibody (Boster Biological Technology, Wuhan, China. Catalog #BM1381)
应用范围	WB, FC, ELISA
抗体来源	Mouse
抗体亚型	IgG1
免疫原	Purified recombinant fragment of human RAF1 expressed in E. Coli.
抗体规格	200ul/1180元

产品详情

克隆	Monoclonal
克隆号	单克隆抗体：IgG1；克隆号：4G4
产品形态	Liquid
内容	Ascitic fluid containing 0.03% sodium azide.
储存条件	连续使用时4℃存储，保质期六个月；长期存储时建议分装为10ul以上小包装-20℃存储，并避免反复冻融，保质期一年。

指标相关信息 (来源: Uniprot.org)

小贴士：你可以利用tissue specificity信息来帮助设计阳性和阴性对照。对此有疑问请联系我们技术服务部门。

基因名	RAF1
基因全名	RAF proto-oncogene serine/threonine-protein kinase
蛋白功能	In skeletal muscle, isoform 1 is more abundant than isoform 2. .
Tissue Specificity	Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2- antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation. .
Sequence Similarities	RAF proto-oncogene serine/threonine-protein kinase;2.7.11.1;Proto-oncogene c-RAF;cRaf;Raf-1;RAF1;RAF;
Uniprot ID	RAF1: P04049

指标背景简介

This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.

产品应用细节

为了提供最优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。

应用	稀释度*
Western blotting	1:100-400
Immunohistochemistry in paraffin section	1:50-200
Immunocytochemistry in fixed cells	1:50-200
Flow Cytometry	1:40-80
enzyme-linked immuno sorbent assay	1:2000

*最优稀释度需要用户自己调试，此处数据仅供参考。

**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。

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