Anti-PAK2 Antibody

免疫原 ：0

亚型 ：0

形态 ：0

保存条件 ：0

克隆性 ：单克隆

适应物种 ：human,monkey

抗原来源 ：0

库存 ：5-7天

供应商 ：博士德生物

应用范围 ：WB,IHC-P,IC,ELISA

浓度 ：0

规格 ：200ul

产品概况

产品名称	PAK2 Antibody
产品描述	Anti-PAK2 Antibody validated for IHC, WB, ELISA in Human, Monkey.
文献引用格式	PAK2 Antibody (Boster Biological Technology, Wuhan, China. Catalog #BM2043)
应用范围	IHC, WB, ELISA
抗体来源	Mouse
抗体亚型	IgG1
免疫原	Purified recombinant fragment of PAK2 expressed in E. Coli.
抗体规格	200ul/1180元

产品详情

克隆	Monoclonal
克隆号	单克隆抗体：IgG1；克隆号：3B5
产品形态	Liquid
内容	Ascitic fluid containing 0.03% sodium azide.
储存条件	连续使用时4℃存储，保质期六个月；长期存储时建议分装为10ul以上小包装-20℃存储，并避免反复冻融，保质期一年。

指标相关信息 (来源: Uniprot.org)

小贴士：你可以利用tissue specificity信息来帮助设计阳性和阴性对照。对此有疑问请联系我们技术服务部门。

基因名	PAK2
基因全名	Serine/threonine-protein kinase PAK 2
蛋白功能	Ubiquitously expressed. Higher levels seen in skeletal muscle, ovary, thymus and spleen.
Tissue Specificity	Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell motility, cell cycle progression, apoptosis or proliferation. Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Full-length PAK2 stimulates cell survival and cell growth. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Phosphorylates JUN and plays an important role in EGF- induced cell proliferation. Phosphorylates many other substrates including histone H4 to promote assembly of H3.3 and H4 into nucleosomes, BAD, ribosomal protein S6, or MBP. Additionally, associates with ARHGEF7 and GIT1 to perform kinase-independent functions such as spindle orientation control during mitosis. On the other hand, apoptotic stimuli such as DNA damage lead to caspase-mediated cleavage of PAK2, generating PAK-2p34, an active p34 fragment that translocates to the nucleus and promotes cellular apoptosis involving the JNK signaling pathway. Caspase- activated PAK2 phosphorylates MKNK1 and reduces cellular translation. .
Sequence Similarities	Serine/threonine-protein kinase PAK 2;2.7.11.1;Gamma-PAK;PAK65;S6/H4 kinase;p21-activated kinase 2;PAK-2;p58;PAK-2p27;p27;PAK-2p34;p34;C-t-PAK2;PAK2;
Uniprot ID	PAK2: Q13177

指标背景简介

PAK2, also known as P21 (CDKN1A)-activated kinase 2. The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. The PAK proteins are a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. PAK2 is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell. PAK2 has been shown to interact with SH3KBP1, CDC42 and Abl gene.

产品应用细节

为了提供最优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。

应用	稀释度*
Western blotting	1:100-400
Immunohistochemistry in paraffin section	1:50-200
Immunocytochemistry in fixed cells	1:50-200
Flow Cytometry	1:40-80
enzyme-linked immuno sorbent assay	1:2000

*最优稀释度需要用户自己调试，此处数据仅供参考。

**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。

产品图片

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[list_product_images]PAK2(BM2043)(MW:61KD)Hela，Jurkat，A549，HEK293，K562细胞裂解，免疫印迹分析。|Immunohistochemical analysis of paraffin-embedded human lung cancer (left) and gastric cancer (right) using PAK2 mouse mAb with DAB staining.[/list_product_images]