Anti-Glucocorticoid receptor(GR) Antibody

价格： ￥1180

品牌：BOSTER 品牌认证

货号：BM3651

产品详情

产品概况

产品名称	Glucocorticoid receptor(GR) Antibody
产品描述	Anti-Glucocorticoid receptor(GR) Antibody validated for IHC, WB, FC, ELISA in Human.
文献引用格式	Glucocorticoid receptor(GR) Antibody (Boster Biological Technology, Wuhan, China. Catalog #BM3651)
应用范围	IHC, WB, FC, ELISA
抗体来源	Mouse
抗体亚型	IgG1
免疫原	Purified recombinant fragment of human NR3C1 expressed in E. Coli.
抗体规格	200ul/1180元

产品详情

克隆	Monoclonal
克隆号	单克隆抗体：IgG1；克隆号：6E6
产品形态	Liquid
内容	Ascitic fluid containing 0.03% sodium azide.
储存条件	连续使用时4℃存储，保质期六个月；长期存储时建议分装为10ul以上小包装-20℃存储，并避免反复冻融，保质期一年。

指标相关信息 (来源: Uniprot.org)

小贴士：你可以利用tissue specificity信息来帮助设计阳性和阴性对照。对此有疑问请联系我们技术服务部门。

基因名	NR3C1
基因全名	Glucocorticoid receptor
蛋白功能	Widely expressed. In the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart. .
Tissue Specificity	Receptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. Involved in chromatin remodeling. May play a negative role in adipogenesis through the regulation of lipolytic and antilipogenic genes expression. .
Sequence Similarities	Glucocorticoid receptor;GR;Nuclear receptor subfamily 3 group C member 1;NR3C1;GRL;
Uniprot ID	NR3C1: P04150

指标背景简介

The protein encoded by this gene is a receptor for glucocorticoids that can act as both a transcription factor and as a regulator of other transcription factors. This protein can also be found in heteromeric cytoplasmic complexes along with heat shock factors and immunophilins. The protein is typically found in the cytoplasm until it binds a ligand, which induces transport into the nucleus. Mutations in this gene are a cause of glucocorticoid resistance, or cortisol, resistance. Tissue specificity: Widely expressed. In the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart.

产品应用细节

为了提供最优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。

应用	稀释度*
Western blotting	1:100-400
Immunohistochemistry in paraffin section	1:50-200
Immunocytochemistry in fixed cells	1:50-200
Flow Cytometry	1:40-80
enzyme-linked immuno sorbent assay	1:2000

*最优稀释度需要用户自己调试，此处数据仅供参考。

**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。

产品图片

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[list_product_images]NR3C1(BM3651)(MW:95KD)Hela细胞裂解，免疫印迹分析。|Immunohistochemical analysis of paraffin-embedded lung cancer tissues using NR3C1 mouse mAb with DAB staining.|Flow cytometric analysis of K562 cells using NR3C1 mouse mAb (green) and negative control (purple).|Red: Control Antigen (100ng);Purple: Antigen (10ng);Green: Antigen (50ng);Blue: Antigen (100ng);[/list_product_images]