Anti-KDM1A(LSD1 AOF2) Antibody

免疫原 ：0

亚型 ：0

形态 ：0

保存条件 ：0

克隆性 ：单克隆

适应物种 ：human,mouse,monkey

抗原来源 ：0

库存 ：5-7天

供应商 ：博士德生物

应用范围 ：WB,IHC-P,ELISA

浓度 ：0

规格 ：200ul

产品概况

产品名称	KDM1A(LSD1 AOF2) Antibody
产品描述	Anti-KDM1A(LSD1 AOF2) Antibody validated for IHC, WB, ELISA in Human, Mouse, Monkey.
文献引用格式	KDM1A(LSD1 AOF2) Antibody (Boster Biological Technology, Wuhan, China. Catalog #BM1974)
应用范围	IHC, WB, ELISA
抗体来源	Mouse
抗体亚型	IgG1
免疫原	Purified recombinant fragment of human LSD1 expressed in E. Coli.
抗体规格	200ul/1180元

产品详情

克隆	Monoclonal
克隆号	单克隆抗体；IgG1；克隆号：1B2E5
产品形态	Liquid
内容	Ascitic fluid containing 0.03% sodium azide.
储存条件	连续使用时4℃存储，保质期六个月；长期存储时建议分装为10ul以上小包装-20℃存储，并避免反复冻融，保质期一年。

指标相关信息 (来源: Uniprot.org)

小贴士：你可以利用tissue specificity信息来帮助设计阳性和阴性对照。对此有疑问请联系我们技术服务部门。

基因名	KDM1A
基因全名	Lysine-specific histone demethylase 1A
蛋白功能	Ubiquitously expressed. .
Tissue Specificity	Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr- 6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Effector of SNAI1-mediated transcription repression of E- cadherin/CDH1, CDN7 and KRT8. Required for the maintenance of the silenced state of the SNAI1 target genes E-cadherin/CDH1 and CDN7. .
Sequence Similarities	Lysine-specific histone demethylase 1A;1.-.-.-;BRAF35-HDAC complex protein BHC110;Flavin-containing amine oxidase domain-containing protein 2;KDM1A;AOF2, KDM1, KIAA0601, LSD1;
Uniprot ID	KDM1A: O60341

指标背景简介

The amine oxidase domain 2 (AOF2) gene encodes a nuclear protein (LSD1, ~95kDa) containing a Swirm domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. LSD1 is a chromatin-modifying enzyme, which serve as a docking module for the stabilization of the associated corepressor complex (es) on chromatin.

产品应用细节

为了提供最优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。

应用	稀释度*
Western blotting	1:100-400
Immunohistochemistry in paraffin section	1:50-200
Immunocytochemistry in fixed cells	1:50-200
Flow Cytometry	1:40-80
enzyme-linked immuno sorbent assay	1:2000

*最优稀释度需要用户自己调试，此处数据仅供参考。

**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。

产品图片

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[list_product_images]LSD1(BM1974)(MW:93KD)COS7，Hela，NIH3T3，A549，Jurkat细胞裂解，免疫印迹分析。|Immunohistochemical analysis of paraffin-embedded human lung carcinoma (left) and kidney carcinoma (right), showing nuclear localization using LSD1 mouse mAb with DAB staining.[/list_product_images]