Anti-KI67 Antibody

免疫原 ：0

亚型 ：0

形态 ：0

保存条件 ：0

克隆性 ：单克隆

适应物种 ：human

抗原来源 ：0

库存 ：5-7天

供应商 ：博士德生物

应用范围 ：WB,IHC-P,ELISA

浓度 ：0

规格 ：200ul

产品概况

产品名称	KI67 Antibody
产品描述	Anti-KI67 Antibody validated for IHC, WB, ELISA in Human.
文献引用格式	KI67 Antibody (Boster Biological Technology, Wuhan, China. Catalog #BM2889)
应用范围	IHC, WB, ELISA
抗体来源	Mouse
抗体亚型	IgG1
免疫原	Synthetic peptide corresponding to aa (CEDLAGFKELFQTPG) of human KI67, conjugated to KLH.
抗体规格	200ul/1180元

产品详情

克隆	Monoclonal
克隆号	单克隆抗体；IgG1；克隆号：8D5
产品形态	Liquid
内容	Ascitic fluid containing 0.03% sodium azide.
储存条件	连续使用时4℃存储，保质期六个月；长期存储时建议分装为10ul以上小包装-20℃存储，并避免反复冻融，保质期一年。

指标相关信息 (来源: Uniprot.org)

小贴士：你可以利用tissue specificity信息来帮助设计阳性和阴性对照。对此有疑问请联系我们技术服务部门。

基因名	MKI67
基因全名	Antigen KI-67
Tissue Specificity	Thought to be required for maintaining cell proliferation.
Sequence Similarities	Antigen KI-67;MKI67;
Uniprot ID	MKI67: P46013

指标背景简介

Ki67, also known as MKI67, it is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation.

产品应用细节

为了提供最优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。

应用	稀释度*
Western blotting	1:100-400
Immunohistochemistry in paraffin section	1:50-200
Immunocytochemistry in fixed cells	1:50-200
Flow Cytometry	1:40-80
enzyme-linked immuno sorbent assay	1:2000

*最优稀释度需要用户自己调试，此处数据仅供参考。

**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。

产品图片

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[list_product_images]KI67(BM2889)(MW:358KD)Hela ，MCF-7，Raji细胞裂解，免疫印迹分析。|Immunohistochemical analysis of paraffin-embedded lung cancer (left) and rectal cancer (right) using KI67 mouse mAb with DAB staining.[/list_product_images]